FastQCFastQC Report
Fri 10 Feb 2017
ERR1631484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11823
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3703.129493360399222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3272.765795483379853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2472.0891482703205613No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1381.1672164425272773No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA780.659731032732809No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.42290450816205705No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA430.36369787701936906No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA400.3383236065296456No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.3129493360399222No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.3129493360399222No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.304491245876681No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA340.28757506555019874No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.27065888522371645No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG290.24528461473399304No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT280.2368265245707519No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA280.2368265245707519No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA270.2283684344075108No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC270.2283684344075108No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT270.2283684344075108No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA250.21145225408102852No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA240.20299416391778738No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC220.1860779835913051No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1860779835913051No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT220.1860779835913051No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT210.17761989342806395No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC210.17761989342806395No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.17761989342806395No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA210.17761989342806395No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT190.16070371310158166No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.14378753277509937No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT170.14378753277509937No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA170.14378753277509937No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGAGGGGAAA170.14378753277509937ABI Solid3 Adapter B (95% over 23bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.14378753277509937No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA160.13532944261185822No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT160.13532944261185822No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT160.13532944261185822No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC160.13532944261185822No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG160.13532944261185822No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT160.13532944261185822No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC150.12687135244861708No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA140.11841326228537595ABI Solid3 Adapter B (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT140.11841326228537595No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT140.11841326228537595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC130.10995517212213483No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA130.10995517212213483No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.10995517212213483No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA130.10995517212213483No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC130.10995517212213483No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT120.10149708195889369No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA120.10149708195889369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC500.00664034918.49999837
GGTATCA1701.8189894E-1217.4117641
ATAGAGA854.8274914E-415.2352944
AGAGAGG854.8274914E-415.2352946
TAGAGAG854.8274914E-415.2352945
AACGCAG3000.014.1833335
CATAGAG950.001122203113.63157753
CTCTATG1102.1497415E-413.4545451
TCTATGG1153.1333184E-412.8695642
ACGCAGA3450.012.3333346
CCCATAG1050.002386259412.3333331
CGCAGAG3300.012.3333337
CCATAGA1050.002386259412.3333332
CAGAGTA3250.011.9538479
AGAGTAC3250.011.95384710
GCAGAGT3250.011.9538478
GTATCAA2551.7826096E-911.6078432
TCAACGC3750.011.3466673
CAACGCA3750.011.3466674
AGTACTT2153.7488826E-711.18604712
Produced by FastQC (version 0.11.5)