##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11823
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29341114776283	33.0	31.0	34.0	30.0	34.0
2	32.39786856127886	34.0	31.0	34.0	31.0	34.0
3	32.35566269136429	34.0	31.0	34.0	30.0	34.0
4	36.00143787532775	37.0	35.0	37.0	35.0	37.0
5	35.91161295779413	37.0	35.0	37.0	35.0	37.0
6	36.04668865770109	37.0	35.0	37.0	35.0	37.0
7	36.043982068848855	37.0	35.0	37.0	35.0	37.0
8	36.099297978516454	37.0	35.0	37.0	35.0	37.0
9	37.77966675124757	39.0	37.0	39.0	35.0	39.0
10	37.69838450477882	39.0	37.0	39.0	35.0	39.0
11	37.78956271673856	39.0	37.0	39.0	35.0	39.0
12	37.58174744142772	39.0	37.0	39.0	35.0	39.0
13	37.74152076461135	39.0	37.0	39.0	35.0	39.0
14	38.9018861541064	40.0	38.0	41.0	36.0	41.0
15	38.79049310665652	40.0	38.0	41.0	36.0	41.0
16	38.663368011503	40.0	38.0	41.0	35.0	41.0
17	38.78567199526347	40.0	38.0	41.0	36.0	41.0
18	38.93842510361161	40.0	38.0	41.0	36.0	41.0
19	38.97902393639516	40.0	38.0	41.0	36.0	41.0
20	38.99873128647551	40.0	39.0	41.0	36.0	41.0
21	38.99010403450901	40.0	38.0	41.0	35.0	41.0
22	38.899433307959065	40.0	38.0	41.0	35.0	41.0
23	38.7737460881333	40.0	38.0	41.0	35.0	41.0
24	38.69060306182864	40.0	38.0	41.0	35.0	41.0
25	38.73170938002199	40.0	38.0	41.0	35.0	41.0
26	38.45267698553666	40.0	38.0	41.0	35.0	41.0
27	38.316840057515016	40.0	37.0	41.0	35.0	41.0
28	38.148185739659986	40.0	37.0	41.0	34.0	41.0
29	37.816713186162566	40.0	36.0	41.0	34.0	41.0
30	37.54613888184048	39.0	35.0	41.0	33.0	41.0
31	37.218218726211624	39.0	35.0	41.0	33.0	41.0
32	36.946883193774845	38.0	35.0	41.0	33.0	41.0
33	36.627336547407594	38.0	35.0	40.0	33.0	41.0
34	36.39034086103358	38.0	35.0	40.0	32.0	41.0
35	36.16459443457667	38.0	35.0	40.0	31.0	41.0
36	35.83904254419352	38.0	35.0	40.0	30.0	41.0
37	35.63038145986636	38.0	35.0	40.0	30.0	41.0
38	35.293072824156305	38.0	35.0	40.0	27.0	41.0
39	34.926076291973274	37.0	35.0	40.0	25.0	41.0
40	34.55459697200372	37.0	35.0	40.0	22.0	41.0
41	34.09177027827116	37.0	34.0	40.0	18.0	41.0
42	33.616425611097014	37.0	33.0	40.0	15.0	41.0
43	32.47449885815783	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	2.0
21	6.0
22	8.0
23	13.0
24	24.0
25	39.0
26	60.0
27	82.0
28	128.0
29	187.0
30	245.0
31	250.0
32	297.0
33	416.0
34	503.0
35	734.0
36	1172.0
37	2371.0
38	2817.0
39	2466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.51103780766303	15.444472638078322	17.178381121542756	20.866108432715894
2	19.56356254757676	23.395077391524993	32.901970735008035	24.139389325890214
3	20.92531506385858	25.09515351433646	31.44717922693056	22.5323521948744
4	13.989681130000845	19.639685359045927	30.313795145056243	36.05683836589698
5	13.279201556288589	40.556542332741266	30.017761989342805	16.146494121627335
6	26.676816374862554	30.787448194197754	20.798443711409963	21.73729171952973
7	25.991711071640022	34.9911190053286	19.352110293495727	19.66505962953565
8	34.187600439820685	29.112746341876004	18.134145309989005	18.565507908314302
9	29.983929628689843	12.898587498942737	18.455552736192168	38.66193013617526
10	22.439313203078743	29.197327243508415	22.84530153091432	25.51805802249852
11	30.499873128647554	26.363867038822637	16.036538949505204	27.099720883024613
12	22.79455298993487	33.56170176774084	24.875243170092194	18.768502072232092
13	35.625475767571686	18.768502072232092	26.57531929290366	19.030702867292565
14	23.056753784995347	23.327412670219065	28.613719022244776	25.002114522540808
15	30.736699653218302	24.90907553074516	23.987143702951876	20.367081113084666
16	21.111393047449887	26.177789055231326	29.628689841833715	23.08212805548507
17	16.92463841664552	32.62285375962108	28.376892497674028	22.075615326059374
18	19.123741859088216	23.26820603907638	33.32487524317009	24.283176858665314
19	20.181003129493362	29.171952973018694	34.52592404635033	16.121119851137614
20	23.107502325974792	23.44582593250444	36.3697877019369	17.076884039583863
21	26.431531760128564	21.982576334263722	33.42637232512898	18.15951958047873
22	23.631903916095744	24.33392539964476	30.49141503848431	21.542755645775184
23	20.747695170430518	25.788716907722236	31.954664636725028	21.50892328512222
24	18.058022498519836	27.45496066988074	34.8473314725535	19.639685359045927
25	18.514759367334857	26.41461557980208	32.42831768586653	22.642307366996533
26	22.126363867038823	24.807578448786263	32.783557472722656	20.282500211452252
27	19.56356254757676	25.695677915926584	31.303391694155458	23.4373678423412
28	18.667004990273195	25.594180833967688	35.04186754630804	20.69694662945107
29	18.5232174574981	26.922100989596547	35.625475767571686	18.92920578533367
30	17.356001014970822	27.615664382982324	37.469339423158246	17.558995178888605
31	18.03264822803011	29.5441089402013	33.747779751332146	18.675463080436437
32	17.178381121542756	29.594857481180746	36.17525162818236	17.05150976909414
33	17.78736361329612	27.674871014125014	36.741943669119514	17.795821703459357
34	18.675463080436437	28.99433307959063	31.362598325298148	20.967605514674787
35	16.933096506808763	30.27996278440328	32.11536834982661	20.671572358961345
36	15.030026220079506	29.67943838281316	33.553243677577605	21.73729171952973
37	16.86543178550283	29.66252220248668	33.3671656939863	20.10488031802419
38	16.78085088387042	26.389241309312357	35.515520595449544	21.314387211367674
39	17.609743719868053	26.32157658800643	35.42248160365389	20.646198088471625
40	17.06842594942062	26.71064873551552	33.63782457921001	22.583100735853844
41	13.5075699906961	27.97936226000169	35.7523471200203	22.76072062928191
42	13.922016408694917	27.07434661253489	34.881163833206465	24.12247314556373
43	13.346866277594518	29.628689841833715	35.74388902985706	21.280554850714708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	12.5
4	22.0
5	22.0
6	46.5
7	71.0
8	70.5
9	70.0
10	118.5
11	167.0
12	167.0
13	298.0
14	429.0
15	598.0
16	767.0
17	656.0
18	545.0
19	545.0
20	608.0
21	671.0
22	462.5
23	254.0
24	226.0
25	198.0
26	198.0
27	163.5
28	129.0
29	147.0
30	165.0
31	170.5
32	176.0
33	176.0
34	156.5
35	137.0
36	147.5
37	158.0
38	185.5
39	213.0
40	213.0
41	270.0
42	327.0
43	410.5
44	494.0
45	678.5
46	863.0
47	863.0
48	1101.5
49	1340.0
50	1238.0
51	1136.0
52	874.0
53	612.0
54	612.0
55	508.0
56	404.0
57	376.5
58	349.0
59	369.5
60	390.0
61	390.0
62	390.0
63	390.0
64	355.5
65	321.0
66	301.0
67	281.0
68	281.0
69	265.5
70	250.0
71	230.0
72	210.0
73	168.5
74	127.0
75	127.0
76	98.5
77	70.0
78	53.0
79	36.0
80	31.0
81	26.0
82	26.0
83	19.0
84	12.0
85	9.0
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	11823.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.62699822380107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.10787172011662	63.435676224308544
2	4.701166180758017	6.546561786348643
3	1.3726919339164236	2.8672925653387464
4	0.8138969873663752	2.2667681637486257
5	0.44946550048590866	1.564746680199611
6	0.3522837706511176	1.4717076884039584
7	0.23080660835762876	1.1249259917110717
8	0.09718172983479105	0.5413177704474329
9	0.10932944606413994	0.6851053032225324
>10	0.7045675413022352	9.684513236911107
>50	0.012147716229348881	0.659731032732809
>100	0.048590864917395525	9.151653556626913
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	370	3.129493360399222	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	327	2.765795483379853	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	247	2.0891482703205613	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	138	1.1672164425272773	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	78	0.659731032732809	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.42290450816205705	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	43	0.36369787701936906	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	40	0.3383236065296456	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.3129493360399222	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.3129493360399222	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.304491245876681	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	34	0.28757506555019874	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.27065888522371645	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	29	0.24528461473399304	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	28	0.2368265245707519	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	28	0.2368265245707519	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.2283684344075108	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	27	0.2283684344075108	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	27	0.2283684344075108	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	25	0.21145225408102852	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	24	0.20299416391778738	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	22	0.1860779835913051	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.1860779835913051	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	22	0.1860779835913051	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	21	0.17761989342806395	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	21	0.17761989342806395	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.17761989342806395	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	21	0.17761989342806395	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	19	0.16070371310158166	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.14378753277509937	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	17	0.14378753277509937	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	17	0.14378753277509937	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGAGGGGAAA	17	0.14378753277509937	ABI Solid3 Adapter B (95% over 23bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.14378753277509937	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	16	0.13532944261185822	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	16	0.13532944261185822	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	16	0.13532944261185822	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC	16	0.13532944261185822	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	16	0.13532944261185822	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	16	0.13532944261185822	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	15	0.12687135244861708	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	14	0.11841326228537595	ABI Solid3 Adapter B (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	14	0.11841326228537595	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	14	0.11841326228537595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	13	0.10995517212213483	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	13	0.10995517212213483	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.10995517212213483	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	13	0.10995517212213483	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC	13	0.10995517212213483	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	12	0.10149708195889369	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA	12	0.10149708195889369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.00845809016324114	0.0
17	0.0	0.0	0.0	0.01691618032648228	0.0
18	0.0	0.0	0.0	0.02537427048972342	0.0
19	0.0	0.0	0.0	0.05074854097944684	0.0
20	0.0	0.0	0.0	0.06766472130592913	0.0
21	0.0	0.0	0.0	0.06766472130592913	0.0
22	0.0	0.0	0.0	0.10995517212213482	0.0
23	0.0	0.0	0.0	0.16070371310158166	0.0
24	0.0	0.0	0.0	0.304491245876681	0.0
25	0.0	0.0	0.0	0.3467816966928867	0.0
26	0.0	0.0	0.0	0.3806140573458513	0.0
27	0.0	0.0	0.0	0.5074854097944684	0.0
28	0.0	0.0	0.0	0.7950604753446672	0.0
29	0.0	0.0	0.0	1.1587583523640361	0.0
30	0.0	0.0	0.0	1.9622769178719446	0.0
31	0.0	0.0	0.0	2.9518734669711577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	50	0.006640349	18.499998	37
GGTATCA	170	1.8189894E-12	17.411764	1
ATAGAGA	85	4.8274914E-4	15.235294	4
AGAGAGG	85	4.8274914E-4	15.235294	6
TAGAGAG	85	4.8274914E-4	15.235294	5
AACGCAG	300	0.0	14.183333	5
CATAGAG	95	0.0011222031	13.6315775	3
CTCTATG	110	2.1497415E-4	13.454545	1
TCTATGG	115	3.1333184E-4	12.869564	2
ACGCAGA	345	0.0	12.333334	6
CCCATAG	105	0.0023862594	12.333333	1
CGCAGAG	330	0.0	12.333333	7
CCATAGA	105	0.0023862594	12.333333	2
CAGAGTA	325	0.0	11.953847	9
AGAGTAC	325	0.0	11.953847	10
GCAGAGT	325	0.0	11.953847	8
GTATCAA	255	1.7826096E-9	11.607843	2
TCAACGC	375	0.0	11.346667	3
CAACGCA	375	0.0	11.346667	4
AGTACTT	215	3.7488826E-7	11.186047	12
>>END_MODULE