FastQCFastQC Report
Fri 10 Feb 2017
ERR1631483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460328
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176933.843563719782416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146653.1857718843954745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124822.7115448115256946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56481.2269512173928157No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA30900.6712604925183782No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22130.48074416503015244No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA19120.41535600702108066No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA17330.37647069046419074No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.3664778158182861No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.35887454163118476No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA14900.3236822439651727No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14760.32064093429033214No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14420.3132548965085765No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.2754557619784154No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11760.2554700126866061No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA11660.2532976486331485No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA11460.24895292052623347No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA11150.2422185919605151No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT10750.23352913574668496No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.23048782607184445No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT10180.22114666064197702No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT10080.2189742965885195No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT10020.21767087815644498No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC9640.20941589475330633No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC9590.20832971272657758No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT9420.20463669383569977No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.20094367494482196No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA8400.18247858049043292No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT8310.18052345284232113No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC8220.17856832519420934No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG7800.1694443961696877No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.1535861385794477No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG6830.1483724648511496No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.14772075563511236No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6780.14728628282442083No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.14663457360838358No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT6650.14446220955492606No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA6400.13903129942128223No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA5940.12903842477537755No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA5480.1190455501294729No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT5370.11665594967066961No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT5060.10992162110495125No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT5010.10883543907822249No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT5010.10883543907822249No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT4850.10535965659269042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4740.10297005613388714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC308.301153E-637.012
ACATGTA200.0018407937.03
ATGCCCC2150.028.39534837
CGTATGC405.9325128E-527.7511
TCGTATG405.9325128E-527.7510
ATTGACT2150.027.53488331
AATCTCG509.075633E-625.9000026
TATTGAC2300.024.93478430
AAATCTC451.321529E-424.6666665
CTCGTAT451.321529E-424.6666669
TAAGAGG400.001929438223.1256
TTCTGCT502.6986928E-422.222
AGCGTCA855.163747E-821.7647063
CGTTCCC603.7200407E-521.5833349
TTGACTG2850.021.42105332
TGACTGT2950.020.69491633
TGGGCAG23300.020.6437786
CGGGACG450.00382237620.5555554
ATACAAC450.00382237620.5555551
CAGTCGG23000.020.26956610
Produced by FastQC (version 0.11.5)