FastQCFastQC Report
Fri 10 Feb 2017
ERR1631482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816662
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.37861440840886434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26400.32326715336332534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22900.28040976560682385No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12320.15085800490288515No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10390.1272252167971572No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10150.12428642449385426No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9530.11669454437698829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.10824551650499227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG358.8671886E-426.4285710
TACGTTA509.086883E-625.89999819
GGTATCA14200.025.6654931
ACGTTAA451.322754E-424.66666620
ATTACGT551.9017845E-523.54545417
TTACGTT603.7245853E-521.58333418
AGTCGGT4800.021.19791811
GCAGTCG5000.020.7200019
TCGGTGA5000.020.72000113
CAGTCGG5000.020.35000210
CGGTGAT5200.020.27884714
GCCTTAT3700.020.026
TCGTTTA1700.019.58823430
GTGATTC5400.019.52777716
GGCAGTC5350.019.3644858
TTCCTCG3950.019.20253220
GTCGGTG5600.019.16071312
CTTAACG609.233693E-418.530
TTAGAAC801.6157363E-518.53
AACGCTA609.233693E-418.533
Produced by FastQC (version 0.11.5)