##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	816662
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.773034621422326	34.0	31.0	34.0	31.0	34.0
2	32.94509356379996	34.0	33.0	34.0	31.0	34.0
3	33.05974687202294	34.0	33.0	34.0	31.0	34.0
4	36.41951505029008	37.0	37.0	37.0	35.0	37.0
5	36.39192591304603	37.0	37.0	37.0	35.0	37.0
6	36.43845556668487	37.0	37.0	37.0	35.0	37.0
7	36.43046572511027	37.0	37.0	37.0	35.0	37.0
8	36.417293813107506	37.0	37.0	37.0	35.0	37.0
9	38.25643411839904	39.0	39.0	39.0	37.0	39.0
10	38.2115244740174	39.0	39.0	39.0	37.0	39.0
11	38.2768244389968	39.0	39.0	39.0	37.0	39.0
12	38.051434987791765	39.0	39.0	39.0	37.0	39.0
13	38.18744596907901	39.0	39.0	39.0	37.0	39.0
14	39.679339800309066	41.0	40.0	41.0	37.0	41.0
15	39.6798969463499	41.0	40.0	41.0	37.0	41.0
16	39.65313311994436	41.0	40.0	41.0	37.0	41.0
17	39.648685747592026	41.0	40.0	41.0	37.0	41.0
18	39.64989065243638	41.0	40.0	41.0	37.0	41.0
19	39.66419399947591	41.0	40.0	41.0	37.0	41.0
20	39.6474416588503	41.0	40.0	41.0	37.0	41.0
21	39.62139661206227	41.0	40.0	41.0	37.0	41.0
22	39.571506939223326	41.0	40.0	41.0	37.0	41.0
23	39.503526550763965	41.0	39.0	41.0	37.0	41.0
24	39.469285946940104	41.0	39.0	41.0	37.0	41.0
25	39.45809649524528	41.0	39.0	41.0	37.0	41.0
26	39.38334096603001	41.0	39.0	41.0	36.0	41.0
27	39.317181404301905	41.0	39.0	41.0	36.0	41.0
28	39.229305391949175	41.0	39.0	41.0	36.0	41.0
29	39.15986540331251	41.0	39.0	41.0	35.0	41.0
30	39.10767734999302	40.0	39.0	41.0	35.0	41.0
31	39.04560515856009	40.0	39.0	41.0	35.0	41.0
32	38.96975003122467	40.0	39.0	41.0	35.0	41.0
33	38.862853910185606	40.0	38.0	41.0	35.0	41.0
34	38.81992427711832	40.0	38.0	41.0	35.0	41.0
35	38.714350612615746	40.0	38.0	41.0	35.0	41.0
36	38.66088908263149	40.0	38.0	41.0	35.0	41.0
37	38.59165358495926	40.0	38.0	41.0	35.0	41.0
38	38.50305634399543	40.0	38.0	41.0	35.0	41.0
39	38.40978764776615	40.0	38.0	41.0	35.0	41.0
40	38.30803318876108	40.0	38.0	41.0	34.0	41.0
41	38.214953065037925	40.0	37.0	41.0	34.0	41.0
42	38.09055766032949	40.0	37.0	41.0	34.0	41.0
43	37.178467958592414	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	1.0
14	4.0
15	3.0
16	4.0
17	8.0
18	21.0
19	29.0
20	64.0
21	122.0
22	215.0
23	382.0
24	605.0
25	877.0
26	1316.0
27	1864.0
28	2562.0
29	3813.0
30	5018.0
31	6701.0
32	8701.0
33	11533.0
34	17191.0
35	25323.0
36	41660.0
37	80367.0
38	182599.0
39	425676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27469626357048	18.079940048637013	12.757909637034661	25.887454050757842
2	19.15112984319094	19.67031648344113	33.99680651236374	27.181747161004182
3	19.66358175107939	21.62363376770316	29.26902439442511	29.44376008679233
4	14.179672863436771	15.62310968307574	34.18929740823009	36.0079200452574
5	15.649681263484771	35.432039203489325	32.82043244328743	16.097847089738472
6	34.071378367060056	34.68595330748829	15.993275063612607	15.249393261839048
7	30.507970249625917	30.247397332066388	20.03080833931296	19.213824078994737
8	29.467637774256673	31.092422568945295	19.200721963309178	20.23921769348886
9	27.74575038387975	14.016080091886241	19.199375016836832	39.038794507397185
10	19.129578699633385	25.20871547837416	30.251927970200647	25.409777851791805
11	37.32131530547522	21.338815813641386	20.123502746546308	21.216366134337093
12	23.66523727074359	25.502717158383764	26.69745867935572	24.134586891516932
13	31.530180172458127	18.026307089101735	24.405812931175934	26.037699807264204
14	23.598379745843445	19.922195473770053	23.932300021306244	32.547124759080255
15	27.021338081115566	26.40272230127029	20.96938513118034	25.6065544864338
16	26.157210694265192	24.89181570833466	23.214255101865888	25.736718495534262
17	24.987081558833395	25.52892138975488	23.899238607894084	25.584758443517636
18	25.34181827977793	23.155969054517044	25.74896346346469	25.75324920224034
19	25.80663726241701	24.494588948671545	25.12961298554359	24.569160803367858
20	26.543539432470226	23.103927940812724	24.7074064913024	25.645126135414653
21	27.853383651988217	23.52601198537461	24.180138172218125	24.44046619041905
22	27.577137175477738	23.153887409968874	24.11597454026268	25.15300087429071
23	25.970719832684757	23.185356977550075	24.621936615148005	26.221986574617162
24	24.86928496734267	24.20450565839968	25.14785798775993	25.778351386497718
25	25.46194141517543	23.418256267586834	24.828509224134343	26.29129309310339
26	25.317818142634284	24.42222118820271	25.025530758134945	25.234429911028062
27	26.097700150123305	23.393766331725978	24.541732075203697	25.96680144294702
28	24.96320387136906	23.886626290925744	25.4267983572151	25.723371480490094
29	24.567691407216206	24.246873247438966	25.85035179792864	25.335083547416186
30	24.368710678346734	24.666263399056156	25.59075847780355	25.374267444793563
31	25.21691960688755	24.394425111000633	24.636018328267998	25.752636953843815
32	24.512099252812057	23.675155694767234	25.319165089106633	26.493579963314073
33	24.156872733150312	23.597522598088318	25.90937254335331	26.336232125408067
34	25.77553504387372	22.75617085158854	25.795249442241712	25.67304466229603
35	25.062755460643448	23.01735601754459	26.283823662665828	25.636064859146135
36	23.68446187039436	23.928993879965027	26.18451697275005	26.20202727689056
37	24.625365206168524	22.53159813974447	26.701621968452066	26.14141468563494
38	23.695237442173138	22.066534257746778	27.569055496643653	26.669172803436425
39	24.084749872040085	21.78918573412256	27.984649708202415	26.14141468563494
40	23.213887752827976	22.07045264748452	28.493673025070347	26.221986574617162
41	22.07902412503582	22.269433376353987	29.596087487846866	26.05545501076333
42	22.69543581065361	22.386862618806802	29.312494030578133	25.605207539961455
43	21.473632910555406	21.816981811324638	29.488821568776313	27.220563709343644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	113.0
2	106.0
3	276.5
4	447.0
5	447.0
6	505.0
7	563.0
8	540.0
9	517.0
10	778.0
11	1039.0
12	1039.0
13	1752.5
14	2466.0
15	4019.5
16	5573.0
17	5303.5
18	5034.0
19	5034.0
20	5843.5
21	6653.0
22	5926.0
23	5199.0
24	6019.5
25	6840.0
26	6840.0
27	7897.0
28	8954.0
29	10751.0
30	12548.0
31	14656.5
32	16765.0
33	16765.0
34	19785.0
35	22805.0
36	26543.0
37	30281.0
38	33157.0
39	36033.0
40	36033.0
41	38956.5
42	41880.0
43	44314.0
44	46748.0
45	49462.0
46	52176.0
47	52176.0
48	55839.0
49	59502.0
50	59497.5
51	59493.0
52	60478.5
53	61464.0
54	61464.0
55	58814.0
56	56164.0
57	54544.5
58	52925.0
59	51558.5
60	50192.0
61	50192.0
62	47263.5
63	44335.0
64	41621.0
65	38907.0
66	34683.5
67	30460.0
68	30460.0
69	26357.5
70	22255.0
71	18989.5
72	15724.0
73	11813.5
74	7903.0
75	7903.0
76	6354.5
77	4806.0
78	4060.0
79	3314.0
80	2863.0
81	2412.0
82	2412.0
83	1967.0
84	1522.0
85	1320.5
86	1119.0
87	987.5
88	856.0
89	856.0
90	638.5
91	421.0
92	258.5
93	96.0
94	63.5
95	31.0
96	31.0
97	19.5
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	816662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.80592754033812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07838543423163	52.38337079255736
2	5.804523007186322	6.826807187336488
3	1.583942084037964	2.7943555026608604
4	0.8149423963879788	1.9169377404616395
5	0.492118039035918	1.446972887241974
6	0.3254855009827549	1.1484286071733523
7	0.2503062350507261	1.0303643224901489
8	0.19019139049604275	0.8947504902645154
9	0.15367417399259561	0.8133257106566901
>10	1.0878206497064926	12.709603925594756
>50	0.12426375646384225	4.990949268975786
>100	0.08828065251662777	9.895201752553797
>500	0.00481150475707437	1.7578265680405694
>1k	0.001255175154019401	1.3911052439920748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3092	0.37861440840886434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2640	0.32326715336332534	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2290	0.28040976560682385	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1232	0.15085800490288515	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1039	0.1272252167971572	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1015	0.12428642449385426	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	953	0.11669454437698829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.10824551650499227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.224496793042899E-4	0.0
5	0.0	0.0	0.0	1.224496793042899E-4	0.0
6	0.0	0.0	0.0	1.224496793042899E-4	0.0
7	0.0	0.0	0.0	1.224496793042899E-4	0.0
8	0.0	0.0	0.0	1.224496793042899E-4	0.0
9	0.0	0.0	0.0	1.224496793042899E-4	0.0
10	0.0	0.0	0.0	2.448993586085798E-4	0.0
11	0.0	0.0	0.0	3.673490379128697E-4	0.0
12	0.0	0.0	0.0	4.897987172171596E-4	0.0
13	0.0	0.0	0.0	7.346980758257395E-4	0.0
14	0.0	0.0	0.0	0.001224496793042899	0.0
15	0.0	0.0	0.0	0.0015918458309557688	0.0
16	0.0	0.0	0.0	0.002448993586085798	0.0
17	0.0	0.0	0.0	0.0035510406998244073	0.0
18	0.0	0.0	0.0	0.003795940058432987	0.0
19	0.0	0.0	0.0	0.004653087813563017	0.0
20	0.0	0.0	0.0	0.005020436851475886	0.0
21	0.0	0.0	0.0	0.006000034285910205	0.0
22	0.0	0.0	0.0	0.008816376909908873	0.0
23	0.0	0.0	0.0	0.01310211568555902	0.0
24	0.0	0.0	0.0	0.020571546123120704	0.0
25	0.0	0.0	0.0	0.02461238554016227	0.0
26	0.0	0.0	0.0	0.030489970146768186	0.0
27	0.0	0.0	0.0	0.06942896816553237	0.0
28	0.0	1.224496793042899E-4	0.0	0.2327768403574551	0.0
29	0.0	1.224496793042899E-4	0.0	0.4817170383830765	0.0
30	0.0	1.224496793042899E-4	0.0	0.8149026157700493	0.0
31	0.0	1.224496793042899E-4	0.0	1.6391114071672246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCCG	35	8.8671886E-4	26.42857	10
TACGTTA	50	9.086883E-6	25.899998	19
GGTATCA	1420	0.0	25.665493	1
ACGTTAA	45	1.322754E-4	24.666666	20
ATTACGT	55	1.9017845E-5	23.545454	17
TTACGTT	60	3.7245853E-5	21.583334	18
AGTCGGT	480	0.0	21.197918	11
GCAGTCG	500	0.0	20.720001	9
TCGGTGA	500	0.0	20.720001	13
CAGTCGG	500	0.0	20.350002	10
CGGTGAT	520	0.0	20.278847	14
GCCTTAT	370	0.0	20.0	26
TCGTTTA	170	0.0	19.588234	30
GTGATTC	540	0.0	19.527777	16
GGCAGTC	535	0.0	19.364485	8
TTCCTCG	395	0.0	19.202532	20
GTCGGTG	560	0.0	19.160713	12
CTTAACG	60	9.233693E-4	18.5	30
TTAGAAC	80	1.6157363E-5	18.5	3
AACGCTA	60	9.233693E-4	18.5	33
>>END_MODULE