FastQCFastQC Report
Fri 10 Feb 2017
ERR1631481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178894
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4260.23812984225295425No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3050.17049202320927478No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2910.16266615984884905No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2660.14869140384808882No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC2640.147573423368028No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC2320.12968573568705488No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC2300.1285677552069941No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2230.12465482352678123No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT2010.11235703824611222No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1930.10788511632586896No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1860.10397218464565608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1600.031.218751
CAAGACG556.212176E-726.909094
TAAGGCT502.6897606E-422.19999934
AAGACGG603.70369E-521.5833325
GCGCAAG603.70369E-521.5833321
TTTACTC450.00381337820.5555573
GGATGCG450.00381337820.5555575
GCTAATA450.00381337820.5555572
TATACAC555.119792E-420.18181837
CTTATAC1950.019.92307737
GTATCAA2600.019.9230772
CGGACCA656.860571E-519.9230779
GCGAAAG701.2119088E-418.518
CTAAGGC609.1953116E-418.533
GAGCGAA701.2119088E-418.516
ACGGACC701.2119088E-418.58
ACATCTA1601.8189894E-1218.529
CGCAAGA701.2119088E-418.52
TAATACA500.00701224718.4999984
CTAATAC500.00701224718.4999983