##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178894
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75351884356099	34.0	31.0	34.0	31.0	34.0
2	32.939030934519884	34.0	33.0	34.0	31.0	34.0
3	33.052623341196465	34.0	33.0	34.0	31.0	34.0
4	36.408750433217435	37.0	37.0	37.0	35.0	37.0
5	36.3786208592798	37.0	37.0	37.0	35.0	37.0
6	36.4265654521672	37.0	37.0	37.0	35.0	37.0
7	36.41584401936342	37.0	37.0	37.0	35.0	37.0
8	36.39739734144242	37.0	37.0	37.0	35.0	37.0
9	38.24934877637036	39.0	39.0	39.0	37.0	39.0
10	38.18310284302436	39.0	39.0	39.0	37.0	39.0
11	38.26389929231836	39.0	39.0	39.0	37.0	39.0
12	38.01337104654153	39.0	38.0	39.0	37.0	39.0
13	38.15249812738269	39.0	39.0	39.0	37.0	39.0
14	39.636645164175434	41.0	40.0	41.0	37.0	41.0
15	39.65111742148982	41.0	40.0	41.0	37.0	41.0
16	39.62354802285152	41.0	40.0	41.0	37.0	41.0
17	39.61681219045916	41.0	40.0	41.0	37.0	41.0
18	39.5956432300692	41.0	40.0	41.0	37.0	41.0
19	39.60227844421836	41.0	40.0	41.0	37.0	41.0
20	39.59302715574586	41.0	40.0	41.0	37.0	41.0
21	39.54729057430657	41.0	39.0	41.0	37.0	41.0
22	39.49231388419958	41.0	39.0	41.0	37.0	41.0
23	39.40711259181415	41.0	39.0	41.0	37.0	41.0
24	39.37305331648909	41.0	39.0	41.0	36.0	41.0
25	39.349776962894225	41.0	39.0	41.0	36.0	41.0
26	39.28370431652263	41.0	39.0	41.0	36.0	41.0
27	39.206496584569635	41.0	39.0	41.0	36.0	41.0
28	39.095989804018025	40.0	39.0	41.0	35.0	41.0
29	39.013868547855154	40.0	39.0	41.0	35.0	41.0
30	38.96202779299473	40.0	39.0	41.0	35.0	41.0
31	38.91160687334399	40.0	39.0	41.0	35.0	41.0
32	38.848647802609364	40.0	38.0	41.0	35.0	41.0
33	38.744765056402116	40.0	38.0	41.0	35.0	41.0
34	38.68582512549331	40.0	38.0	41.0	35.0	41.0
35	38.580634342124384	40.0	38.0	41.0	35.0	41.0
36	38.52889420550717	40.0	38.0	41.0	35.0	41.0
37	38.44908157903563	40.0	38.0	41.0	34.0	41.0
38	38.35955370219236	40.0	38.0	41.0	34.0	41.0
39	38.278623095240754	40.0	37.0	41.0	34.0	41.0
40	38.17685333214082	40.0	37.0	41.0	34.0	41.0
41	38.08337339430054	40.0	37.0	41.0	34.0	41.0
42	37.97400136393619	40.0	37.0	41.0	34.0	41.0
43	37.00157635247689	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	3.0
18	6.0
19	7.0
20	12.0
21	30.0
22	57.0
23	94.0
24	153.0
25	198.0
26	316.0
27	461.0
28	600.0
29	827.0
30	1105.0
31	1443.0
32	2015.0
33	2653.0
34	4183.0
35	6062.0
36	10109.0
37	17935.0
38	42172.0
39	88450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.991972900153165	16.727223942669962	11.832705400963698	27.448097756213173
2	21.774905810144553	18.74350173845965	29.978087582590806	29.503504868804992
3	21.446219549006674	19.015729985354458	28.249689760416786	31.288360705222086
4	15.609802452849172	14.123447404608317	32.090511699665726	38.17623844287679
5	17.486891678871284	34.035797734971545	31.601395239639118	16.87591534651805
6	38.44790769953156	32.30181001039722	14.090466980446523	15.159815309624694
7	32.75347412434179	27.761132290630208	18.40978456516149	21.075609019866512
8	32.13858486030834	28.36595973034311	17.77700761344707	21.718447795901483
9	28.756134917884335	12.481134079398974	16.94523013628182	41.81750086643487
10	20.054892841570986	23.492123827517972	29.0110344673382	27.44194886357284
11	41.2573926459244	19.106845394479414	17.47571187407068	22.160050085525505
12	25.886838015808245	22.87443961228437	24.32613726564334	26.912585106264043
13	35.41203170592642	15.898800406944893	21.23268527731506	27.45648260981363
14	25.55647478395027	18.022404328820418	20.527239594396683	35.893881292832624
15	29.886972173465853	23.960557648663457	18.373450199559514	27.779019978311183
16	29.50070991760484	22.427247420260045	19.958746520285757	28.113296141849364
17	28.459311100428188	22.34116292329536	20.639037642402762	28.56048833387369
18	28.06522298120675	20.234328708620748	22.746430847317406	28.9540174628551
19	29.03283508669939	21.625655416056436	21.402059320044273	27.939450177199905
20	29.95293302178944	19.988931993247398	20.89226022113654	29.165874763826622
21	31.17041376457567	20.926358625778395	20.595995393920422	27.307232215725513
22	31.800395765089938	20.33326998110613	19.99284492492761	27.873489328876317
23	29.535367312486727	20.378548190548592	20.341095844466555	29.74498865249813
24	28.146835556251187	20.917973772177938	21.61000368933558	29.32518698223529
25	28.605766543316154	20.419913468310842	20.697172627365926	30.277147361007078
26	28.373226603463504	21.49149775844914	21.092937717307457	29.042337920779904
27	29.332453855355684	20.00681968092837	21.416593066285063	29.244133397430883
28	28.223976209375383	20.88219839681599	21.478640982928436	29.415184410880187
29	27.20605498228001	21.387525573803483	22.88841436828513	28.51800507563138
30	26.92041096962447	22.49153129786354	21.981173208715777	28.606884523796218
31	28.224535199615413	22.348988786655784	20.949277225619642	28.47719878810916
32	27.50511476069628	21.375786778762844	22.006327769517146	29.112770691023737
33	26.80246402897805	21.231567296834996	22.474202600422597	29.491766073764353
34	28.364841749863047	20.295817635024093	22.9063020559661	28.433038559146755
35	27.321765961966303	20.66475119344416	23.293123302067144	28.720359542522388
36	25.956711795812044	22.391472044898094	23.51560141759925	28.136214741690612
37	27.398347624850473	20.19352242109853	23.920310351381264	28.487819602669738
38	25.967332610372623	20.328239068945855	24.574887922456874	29.129540398224645
39	26.445828255838656	19.406464163135713	25.534674164589088	28.61303341643655
40	25.219962659451966	19.05206435095643	26.541415586883854	29.18655740270775
41	23.635784319205786	19.49757957226067	28.25751562377721	28.609120484756335
42	24.382036289646383	18.56686082261004	29.175377597907143	27.87572528983644
43	22.816863617561236	18.030789182420875	29.32518698223529	29.8271602177826
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	4.0
4	6.0
5	6.0
6	8.5
7	11.0
8	8.5
9	6.0
10	13.0
11	20.0
12	20.0
13	33.5
14	47.0
15	93.0
16	139.0
17	136.5
18	134.0
19	134.0
20	167.0
21	200.0
22	194.5
23	189.0
24	288.5
25	388.0
26	388.0
27	476.5
28	565.0
29	733.0
30	901.0
31	1160.0
32	1419.0
33	1419.0
34	1814.0
35	2209.0
36	2841.0
37	3473.0
38	4039.0
39	4605.0
40	4605.0
41	5260.5
42	5916.0
43	6571.0
44	7226.0
45	8177.5
46	9129.0
47	9129.0
48	10503.5
49	11878.0
50	12259.5
51	12641.0
52	13640.5
53	14640.0
54	14640.0
55	14425.0
56	14210.0
57	14674.0
58	15138.0
59	15219.0
60	15300.0
61	15300.0
62	14878.0
63	14456.0
64	13810.5
65	13165.0
66	11844.5
67	10524.0
68	10524.0
69	8978.0
70	7432.0
71	6355.5
72	5279.0
73	3930.0
74	2581.0
75	2581.0
76	2104.0
77	1627.0
78	1433.5
79	1240.0
80	1092.5
81	945.0
82	945.0
83	770.0
84	595.0
85	444.0
86	293.0
87	248.5
88	204.0
89	204.0
90	152.5
91	101.0
92	67.0
93	33.0
94	25.5
95	18.0
96	18.0
97	12.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	178894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.32845585869994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48014020791571	57.56119249595187
2	5.323596474868738	6.849174816862486
3	1.5382306755626918	2.9685601234029844
4	0.7984845091231232	2.054611019959301
5	0.5698647871894826	1.8329260904073035
6	0.33524072989672615	1.2939311097119939
7	0.27635881734981865	1.2444415188135232
8	0.20851389087658137	1.0730701300143954
9	0.1416694044018613	0.8202036624834103
>10	1.1652800827649907	14.682792452775073
>50	0.11972055463824866	5.308858213677417
>100	0.042899865412039107	4.310238365940224
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	426	0.23812984225295425	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	305	0.17049202320927478	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	291	0.16266615984884905	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	266	0.14869140384808882	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	264	0.147573423368028	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	232	0.12968573568705488	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC	230	0.1285677552069941	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	223	0.12465482352678123	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	201	0.11235703824611222	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	193	0.10788511632586896	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	186	0.10397218464565608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	5.589902400304091E-4	0.0
17	0.0	0.0	0.0	0.0022359609601216362	0.0
18	0.0	0.0	0.0	0.0022359609601216362	0.0
19	0.0	0.0	0.0	0.0022359609601216362	0.0
20	0.0	0.0	0.0	0.0027949512001520455	0.0
21	0.0	0.0	0.0	0.0033539414401824543	0.0
22	0.0	0.0	0.0	0.005030912160273682	0.0
23	0.0	0.0	0.0	0.005589902400304091	0.0
24	0.0	0.0	0.0	0.007825863360425726	0.0
25	0.0	0.0	0.0	0.009502834080516954	0.0
26	0.0	0.0	0.0	0.012856775520699408	0.0
27	0.0	0.0	0.0	0.03242143392176373	0.0
28	0.0	0.0	0.0	0.11962391136650755	0.0
29	0.0	0.0	0.0	0.30800362225675537	0.0
30	0.0	0.0	0.0	0.526009815868615	0.0
31	0.0	0.0	0.0	1.0654353974979598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	160	0.0	31.21875	1
CAAGACG	55	6.212176E-7	26.90909	4
TAAGGCT	50	2.6897606E-4	22.199999	34
AAGACGG	60	3.70369E-5	21.583332	5
GCGCAAG	60	3.70369E-5	21.583332	1
TTTACTC	45	0.003813378	20.555557	3
GGATGCG	45	0.003813378	20.555557	5
GCTAATA	45	0.003813378	20.555557	2
TATACAC	55	5.119792E-4	20.181818	37
CTTATAC	195	0.0	19.923077	37
GTATCAA	260	0.0	19.923077	2
CGGACCA	65	6.860571E-5	19.923077	9
GCGAAAG	70	1.2119088E-4	18.5	18
CTAAGGC	60	9.1953116E-4	18.5	33
GAGCGAA	70	1.2119088E-4	18.5	16
ACGGACC	70	1.2119088E-4	18.5	8
ACATCTA	160	1.8189894E-12	18.5	29
CGCAAGA	70	1.2119088E-4	18.5	2
TAATACA	50	0.007012247	18.499998	4
CTAATAC	50	0.007012247	18.499998	3
>>END_MODULE