FastQCFastQC Report
Fri 10 Feb 2017
ERR1631480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277368
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC45160.3535394655259878No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT34980.27384434242911987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.19352293152795436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21560.16878456325819965No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG19710.154301657783818No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT19230.15054393095803245No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG18180.14232390352662663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18030.14114961389356864No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT17810.1394273224317503No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT16970.1328513004866256No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG16780.13136386695141886No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14760.11555009989290478No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG14300.11194894501819366No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC14030.1098352236786893No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT13660.10693864258381296No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG13430.1051380651464574No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12790.10012776271207671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.024.2154481
CGTAGCG400.001931303823.12500215
TGCATCA10900.021.21559514
CCGCTTA1059.822543E-1021.14285725
CGCTTAT1101.7553248E-920.18181826
AGTCGGT3550.019.80281811
CAGGACA13300.019.7518794
GCTGCAT11950.019.35146512
GACAGGC12350.019.3238877
GCAGTCG3550.019.281699
TCCAGGA14200.019.151412
GCATCAG12150.019.03292315
CTGCATC12400.018.64919313
ACAGGCT12700.018.58
CTTATAC16100.018.38509437
CCAGGAC14400.017.986113
GTTAATA752.0674928E-417.2666662
GGACAGG15000.017.026
ATCAGAA14000.016.6517
CTTATTG4000.016.6528
Produced by FastQC (version 0.11.5)