##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1277368
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77679102654834	34.0	31.0	34.0	31.0	34.0
2	32.9613165509078	34.0	33.0	34.0	31.0	34.0
3	33.0675748883642	34.0	33.0	34.0	31.0	34.0
4	36.419406153903964	37.0	37.0	37.0	35.0	37.0
5	36.39199588528913	37.0	37.0	37.0	35.0	37.0
6	36.431679829148685	37.0	37.0	37.0	35.0	37.0
7	36.4265450520132	37.0	37.0	37.0	35.0	37.0
8	36.408313031170344	37.0	37.0	37.0	35.0	37.0
9	38.255616235885036	39.0	39.0	39.0	37.0	39.0
10	38.21520971247127	39.0	39.0	39.0	37.0	39.0
11	38.27408624609353	39.0	39.0	39.0	37.0	39.0
12	38.05507183521115	39.0	39.0	39.0	37.0	39.0
13	38.17578489519074	39.0	39.0	39.0	37.0	39.0
14	39.6951692855935	41.0	40.0	41.0	37.0	41.0
15	39.69934740810792	41.0	40.0	41.0	38.0	41.0
16	39.67936021569352	41.0	40.0	41.0	37.0	41.0
17	39.66240347339216	41.0	40.0	41.0	37.0	41.0
18	39.59555429602119	41.0	40.0	41.0	37.0	41.0
19	39.6169044472697	41.0	40.0	41.0	37.0	41.0
20	39.59358931803521	41.0	40.0	41.0	37.0	41.0
21	39.57371172598656	41.0	40.0	41.0	37.0	41.0
22	39.51645414633841	41.0	39.0	41.0	37.0	41.0
23	39.45788057944148	41.0	39.0	41.0	37.0	41.0
24	39.42560640316651	41.0	39.0	41.0	36.0	41.0
25	39.409652504211785	41.0	39.0	41.0	36.0	41.0
26	39.31887600127763	41.0	39.0	41.0	36.0	41.0
27	39.25826465043746	41.0	39.0	41.0	36.0	41.0
28	39.18376615039676	41.0	39.0	41.0	35.0	41.0
29	39.11290794821853	41.0	39.0	41.0	35.0	41.0
30	39.06959310081355	40.0	39.0	41.0	35.0	41.0
31	39.035195808882015	40.0	39.0	41.0	35.0	41.0
32	38.964310206612346	40.0	39.0	41.0	35.0	41.0
33	38.86602373004491	40.0	38.0	41.0	35.0	41.0
34	38.8143033174465	40.0	38.0	41.0	35.0	41.0
35	38.70647143188181	40.0	38.0	41.0	35.0	41.0
36	38.66096770860081	40.0	38.0	41.0	35.0	41.0
37	38.59136286489093	40.0	38.0	41.0	35.0	41.0
38	38.51185641099511	40.0	38.0	41.0	35.0	41.0
39	38.436339410412664	40.0	38.0	41.0	35.0	41.0
40	38.32621061432571	40.0	38.0	41.0	34.0	41.0
41	38.230960850749355	40.0	37.0	41.0	34.0	41.0
42	38.12839995991758	40.0	37.0	41.0	34.0	41.0
43	37.190474475640535	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	3.0
14	3.0
15	0.0
16	2.0
17	6.0
18	21.0
19	47.0
20	92.0
21	188.0
22	321.0
23	603.0
24	921.0
25	1331.0
26	2119.0
27	3002.0
28	4175.0
29	5765.0
30	7909.0
31	10705.0
32	13772.0
33	18944.0
34	28988.0
35	44682.0
36	64553.0
37	117840.0
38	279081.0
39	672292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65124224185982	16.689004265019946	11.710172792805205	27.949580700315025
2	20.048646905198815	18.23194255688259	33.77468356808688	27.944726969831716
3	21.070983459739086	20.4162778463215	27.682703809708713	30.8300348842307
4	15.658291111097194	14.509757563990958	31.696112631598726	38.135838693313126
5	17.067986672595524	34.22373192376825	31.47378046107308	17.234500942563148
6	36.98292113157681	31.818943327216587	15.166185468870363	16.03195007233624
7	32.00197593802256	27.914508583274355	19.450933481972303	20.632581996730778
8	29.251319901547557	31.508461148236062	18.401901409773846	20.838317540442535
9	28.355650055426473	13.793049457947907	17.827438921281885	40.023861565343736
10	19.976545521729054	24.41011517432721	29.508254473260642	26.10508483068309
11	38.15243532012701	20.454168258481502	19.29553582053097	22.09786060086052
12	24.972756480513056	23.322488116188914	25.954462613749524	25.750292789548507
13	31.80140726869626	17.296894865066292	23.321783542409076	27.57991432382837
14	24.3869425255682	18.884064733107454	22.761099385611665	33.96789335571269
15	27.812501957149387	24.725137939888896	20.183142211171724	27.27921789178999
16	27.331591209424378	23.496752697734717	21.31680142292589	27.854854669915014
17	26.093889936181274	24.30247195796356	23.092718777987237	26.510919327867928
18	26.139060944066234	22.137316732531268	24.192323590382724	27.53129873301977
19	26.978521459751615	23.005116771361113	23.297280032065938	26.719081736821337
20	26.7676190416544	22.800164087404724	23.38801347771355	27.044203393227324
21	29.311678388686737	22.374523238408976	22.500407087072794	25.81339128583149
22	28.79436466233693	22.594819973570655	22.268210883629465	26.34260448046295
23	27.30215568262239	22.838837359320102	22.595054831497265	27.263952126560238
24	27.009835849966496	22.994626450639128	23.01130136342855	26.98423633596583
25	26.94892936099855	22.181235164807635	23.303229766206762	27.566605707987048
26	27.540223334231012	23.0859078981155	23.545055144641168	25.82881362301232
27	28.193441514113392	22.24002793243607	22.57963249431644	26.986898059134095
28	26.51201533152545	22.04439127956861	24.093683261205854	27.349910127700085
29	26.345735919484444	22.22531016903508	24.427572946872004	27.001380964608472
30	25.671145668280403	22.953134883604413	24.328071471964225	27.047647976150962
31	26.482501518747924	22.93559882508408	23.362727107615033	27.21917254855296
32	25.877820643698605	22.402001615822535	24.01610185944849	27.70407588103037
33	26.388010346274527	22.6835179838543	23.730749478615405	27.197722191255767
34	27.238978900363875	22.529451183997093	23.80003256696582	26.431537348673213
35	27.08295495111824	22.191020911749785	24.519793826054826	26.20623031107715
36	25.952035748507868	22.769475984994145	23.555232321461002	27.723255945036986
37	25.664491360359744	21.713006745119653	24.875838442798003	27.746663451722604
38	25.823020460822566	21.2271639809358	25.616580343330973	27.333235214910662
39	24.91138027569189	21.40009770089747	26.168887900745908	27.519634122664726
40	25.119229540743156	20.661156377801856	27.159440349218077	27.060173732236915
41	23.565644356207454	21.106525292632977	28.023012945368915	27.304817405790654
42	23.825397223039875	21.65883285004791	27.27976589361875	27.236004033293458
43	23.056628943264588	20.079256721633858	28.438398331569285	28.425716003532266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	127.5
2	114.0
3	245.0
4	376.0
5	376.0
6	475.0
7	574.0
8	541.5
9	509.0
10	762.0
11	1015.0
12	1015.0
13	1731.0
14	2447.0
15	3694.5
16	4942.0
17	4622.5
18	4303.0
19	4303.0
20	4952.0
21	5601.0
22	5118.5
23	4636.0
24	5499.0
25	6362.0
26	6362.0
27	7767.0
28	9172.0
29	11471.5
30	13771.0
31	16438.0
32	19105.0
33	19105.0
34	23392.5
35	27680.0
36	33087.5
37	38495.0
38	43201.0
39	47907.0
40	47907.0
41	52654.5
42	57402.0
43	61630.0
44	65858.0
45	70066.5
46	74275.0
47	74275.0
48	77999.5
49	81724.0
50	86374.5
51	91025.0
52	96849.0
53	102673.0
54	102673.0
55	102693.5
56	102714.0
57	97716.5
58	92719.0
59	86738.5
60	80758.0
61	80758.0
62	78902.5
63	77047.0
64	71090.5
65	65134.0
66	57324.0
67	49514.0
68	49514.0
69	42927.0
70	36340.0
71	33323.5
72	30307.0
73	29971.0
74	29635.0
75	29635.0
76	28401.5
77	27168.0
78	21336.5
79	15505.0
80	10139.0
81	4773.0
82	4773.0
83	3576.5
84	2380.0
85	1977.0
86	1574.0
87	1291.5
88	1009.0
89	1009.0
90	757.5
91	506.0
92	309.5
93	113.0
94	78.5
95	44.0
96	44.0
97	29.0
98	14.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1277368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.41270767602888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18664304133114	43.95314748896904
2	6.874060367405245	6.9307999169875245
3	1.954031687210701	2.955240847111517
4	0.8941272709285469	1.803015069379452
5	0.5303381305892996	1.3367890573424996
6	0.3676173267145757	1.1119550896983064
7	0.27946021334623844	0.9861842229753207
8	0.20587222144044093	0.8302860894473306
9	0.15689624338865	0.7118608008077182
>10	1.2538306378104425	12.913087430754045
>50	0.15551724445866877	5.477357128619502
>100	0.12369071598739023	12.914568690061499
>500	0.013241447374351956	4.287316668116244
>1k	0.004673452014477161	3.788391499729987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4516	0.3535394655259878	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3498	0.27384434242911987	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2472	0.19352293152795436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2156	0.16878456325819965	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1971	0.154301657783818	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1923	0.15054393095803245	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1818	0.14232390352662663	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1803	0.14114961389356864	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1781	0.1394273224317503	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1697	0.1328513004866256	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1678	0.13136386695141886	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1476	0.11555009989290478	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1430	0.11194894501819366	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1403	0.1098352236786893	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1366	0.10693864258381296	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1343	0.1051380651464574	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1279	0.10012776271207671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.828597553719836E-5	0.0
4	0.0	0.0	0.0	7.828597553719836E-5	0.0
5	2.348579266115951E-4	0.0	0.0	7.828597553719836E-5	0.0
6	2.348579266115951E-4	0.0	0.0	7.828597553719836E-5	0.0
7	2.348579266115951E-4	0.0	0.0	7.828597553719836E-5	0.0
8	2.348579266115951E-4	0.0	0.0	7.828597553719836E-5	0.0
9	2.348579266115951E-4	0.0	0.0	7.828597553719836E-5	0.0
10	3.1314390214879343E-4	0.0	0.0	7.828597553719836E-5	0.0
11	5.480018287603885E-4	0.0	0.0	7.828597553719836E-5	0.0
12	5.480018287603885E-4	0.0	0.0	7.828597553719836E-5	0.0
13	6.262878042975869E-4	0.0	0.0	7.828597553719836E-5	0.0
14	6.262878042975869E-4	0.0	0.0	1.5657195107439672E-4	0.0
15	6.262878042975869E-4	0.0	0.0	2.348579266115951E-4	0.0
16	6.262878042975869E-4	0.0	0.0	3.914298776859918E-4	0.0
17	7.045737798347853E-4	0.0	0.0	4.697158532231902E-4	0.0
18	7.045737798347853E-4	0.0	0.0	6.262878042975869E-4	0.0
19	7.045737798347853E-4	0.0	0.0	7.045737798347853E-4	0.0
20	7.045737798347853E-4	0.0	0.0	0.0014091475596695706	0.0
21	7.045737798347853E-4	0.0	0.0	0.0018788634128927607	0.0
22	8.61145730909182E-4	0.0	0.0	0.0025834371927275462	0.0
23	8.61145730909182E-4	0.0	0.0	0.004070870727934315	0.0
24	8.61145730909182E-4	7.828597553719836E-5	0.0	0.006497735969587464	0.0
25	8.61145730909182E-4	7.828597553719836E-5	0.0	0.009394317064463803	0.0
26	8.61145730909182E-4	7.828597553719836E-5	0.0	0.01769263047140683	7.828597553719836E-5
27	9.394317064463804E-4	7.828597553719836E-5	0.0	0.050885884099178935	7.828597553719836E-5
28	0.0010177176819835788	7.828597553719836E-5	0.0	0.17074171264662963	7.828597553719836E-5
29	0.001096003657520777	7.828597553719836E-5	0.0	0.3310713905468119	7.828597553719836E-5
30	0.001096003657520777	7.828597553719836E-5	0.0	0.5480801147359258	7.828597553719836E-5
31	0.001096003657520777	7.828597553719836E-5	0.0	1.1100168471419356	7.828597553719836E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1230	0.0	24.215448	1
CGTAGCG	40	0.0019313038	23.125002	15
TGCATCA	1090	0.0	21.215595	14
CCGCTTA	105	9.822543E-10	21.142857	25
CGCTTAT	110	1.7553248E-9	20.181818	26
AGTCGGT	355	0.0	19.802818	11
CAGGACA	1330	0.0	19.751879	4
GCTGCAT	1195	0.0	19.351465	12
GACAGGC	1235	0.0	19.323887	7
GCAGTCG	355	0.0	19.28169	9
TCCAGGA	1420	0.0	19.15141	2
GCATCAG	1215	0.0	19.032923	15
CTGCATC	1240	0.0	18.649193	13
ACAGGCT	1270	0.0	18.5	8
CTTATAC	1610	0.0	18.385094	37
CCAGGAC	1440	0.0	17.98611	3
GTTAATA	75	2.0674928E-4	17.266666	2
GGACAGG	1500	0.0	17.02	6
ATCAGAA	1400	0.0	16.65	17
CTTATTG	400	0.0	16.65	28
>>END_MODULE