FastQCFastQC Report
Fri 10 Feb 2017
ERR1631479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT450.7843820812271222TruSeq Adapter, Index 6 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA410.7146592295624891No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT270.4706292487362733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240.4183371099877985No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA230.4009063970716402No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT220.38347568415548194No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT220.38347568415548194No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA210.36604497123932367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210.36604497123932367No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA200.3486142583231654No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG180.31375283249084884No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT170.29632211957469057No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC170.29632211957469057No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA170.29632211957469057No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC160.2788914066585323No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA160.2788914066585323No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC160.2788914066585323No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT150.261460693742374No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA150.261460693742374No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT150.261460693742374No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT140.2440299808262158No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC140.2440299808262158RNA PCR Primer, Index 6 (95% over 22bp)
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA130.22659926791005752No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT120.20916855499389925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120.20916855499389925No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG110.19173784207774097No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT110.19173784207774097No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT110.19173784207774097No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA110.19173784207774097No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA100.1743071291615827No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC100.1743071291615827No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA90.15687641624542442No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC90.15687641624542442No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC80.13944570332926615No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT80.13944570332926615No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT80.13944570332926615No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT80.13944570332926615No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT70.1220149904131079No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT70.1220149904131079No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT70.1220149904131079No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT70.1220149904131079No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT70.1220149904131079No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA70.1220149904131079No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT70.1220149904131079No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT70.1220149904131079No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC70.1220149904131079No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA70.1220149904131079No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCTG60.10458427749694962No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT60.10458427749694962No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA60.10458427749694962ABI Solid3 Adapter B (100% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC60.10458427749694962No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG60.10458427749694962No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA60.10458427749694962No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCT60.10458427749694962No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAA60.10458427749694962No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT60.10458427749694962No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT60.10458427749694962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGC250.005055446629.634
TATGCCC250.005055446629.636
ATATGCC250.005055446629.635
CTTATTG357.829244E-426.4285728
ATTCCTC357.829244E-426.4285719
GTATCAA451.1117493E-424.6666662
GGTGATT400.001706705923.12515
CAGTCGG400.001706705923.12510
TTATTGA400.001706705923.12529
TGATTCC400.001706705923.12517
AGTCGGT400.001706705923.12511
GTGATTC400.001706705923.12516
GATTCCT400.001706705923.12518
GGTATCA502.2734379E-422.21
GGGCAGT450.003385036720.5555557
GCAGTCG450.003385036720.5555559
CGGTGAT450.003385036720.55555514
GGCAGTC450.003385036720.5555558
GTCGGTG450.003385036720.55555512
AACGCAG554.3331872E-420.1818187
Produced by FastQC (version 0.11.5)