##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5737
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75021788391145	34.0	31.0	34.0	31.0	34.0
2	32.89715879379467	34.0	31.0	34.0	31.0	34.0
3	32.89210388704898	34.0	33.0	34.0	31.0	34.0
4	36.33588983789437	37.0	37.0	37.0	35.0	37.0
5	36.30590901167858	37.0	37.0	37.0	35.0	37.0
6	36.3792923130556	37.0	37.0	37.0	35.0	37.0
7	36.39044796932195	37.0	37.0	37.0	35.0	37.0
8	36.38818197664285	37.0	37.0	37.0	35.0	37.0
9	38.199755970019176	39.0	39.0	39.0	37.0	39.0
10	38.16158270873279	39.0	39.0	39.0	37.0	39.0
11	38.193480913369356	39.0	39.0	39.0	37.0	39.0
12	37.96217535297194	39.0	38.0	39.0	37.0	39.0
13	38.09952937075126	39.0	39.0	39.0	37.0	39.0
14	39.50984835279763	41.0	39.0	41.0	37.0	41.0
15	39.506187903085234	41.0	39.0	41.0	37.0	41.0
16	39.389053512288655	40.0	39.0	41.0	37.0	41.0
17	39.32281680320725	40.0	39.0	41.0	37.0	41.0
18	39.45895067108245	40.0	39.0	41.0	37.0	41.0
19	39.519958166289	41.0	39.0	41.0	37.0	41.0
20	39.5621404915461	41.0	39.0	41.0	37.0	41.0
21	39.52693045145546	41.0	40.0	41.0	37.0	41.0
22	39.50043576782291	41.0	39.0	41.0	37.0	41.0
23	39.4206031026669	41.0	39.0	41.0	37.0	41.0
24	39.32177096043228	41.0	39.0	41.0	36.0	41.0
25	39.33292661669862	41.0	39.0	41.0	36.0	41.0
26	39.223113125326826	41.0	39.0	41.0	36.0	41.0
27	39.12654697577131	41.0	39.0	41.0	35.0	41.0
28	39.104932891755276	40.0	39.0	41.0	35.0	41.0
29	38.90256231479867	40.0	38.0	41.0	35.0	41.0
30	38.79553773749346	40.0	38.0	41.0	35.0	41.0
31	38.629074429144154	40.0	38.0	41.0	35.0	41.0
32	38.56109464877113	40.0	38.0	41.0	35.0	41.0
33	38.380163848701415	40.0	38.0	41.0	34.0	41.0
34	38.27488234268782	40.0	38.0	41.0	34.0	41.0
35	38.20672825518564	40.0	38.0	41.0	34.0	41.0
36	38.085759107547496	40.0	37.0	41.0	34.0	41.0
37	38.002788914066585	40.0	37.0	41.0	34.0	41.0
38	37.842774969496254	40.0	37.0	41.0	33.0	41.0
39	37.54732438556737	40.0	36.0	41.0	33.0	41.0
40	37.45162977165766	40.0	35.0	41.0	33.0	41.0
41	37.24699320202196	39.0	35.0	41.0	33.0	41.0
42	37.09918075649294	39.0	35.0	41.0	33.0	41.0
43	36.1007495206554	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	1.0
22	3.0
23	2.0
24	10.0
25	7.0
26	15.0
27	13.0
28	29.0
29	32.0
30	58.0
31	56.0
32	68.0
33	100.0
34	147.0
35	219.0
36	339.0
37	706.0
38	1422.0
39	2508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00156876416246	13.82255534251351	15.879379466620186	27.296496426703854
2	23.113125326825866	17.448143629074426	31.14868398117483	28.290047062924874
3	18.302248561966184	19.9233048631689	30.99180756492941	30.782639009935508
4	14.397768868746732	18.180233571553075	29.370751263726685	38.051246295973506
5	16.36743942827262	33.53669165068851	30.93951542618093	19.15635349485794
6	33.2926616698623	29.33588983789437	17.273836499912846	20.097611992330485
7	31.061530416594042	29.893672651211432	16.193132299111035	22.851664633083494
8	34.268781593167155	27.139620010458426	16.698622973679623	21.89297542269479
9	29.31845912497821	11.99233048631689	14.31061530416594	44.37859508453896
10	22.328743245598744	22.25902039393411	22.102143977688687	33.31009238277846
11	36.23845215269304	22.799372494335017	14.450061007495208	26.51211434547673
12	24.681889489280113	28.516646330834934	22.032421126024055	24.769043053860905
13	37.47603276974028	16.594038696182675	20.690256231479868	25.239672302597178
14	26.372668642147463	19.766428446923477	20.202196269827436	33.658706641101624
15	35.80268432978909	20.829701934809133	16.576607983266516	26.79100575213526
16	28.2377549241764	22.57277322642496	21.805821858113998	27.383649991284642
17	21.98012898727558	28.725814885828832	20.515949102318285	28.778107024577302
18	27.557957120446225	19.24350705943873	22.625065365173434	30.57347045494161
19	26.233222938818194	27.488234268781593	21.840683283946312	24.437859508453897
20	31.218406832839463	17.971065016559177	24.59473592469932	26.21579222590204
21	35.78525361687293	17.83161931322991	21.387484748126198	24.995642321770962
22	33.36238452152693	17.918772877810703	19.52239846609726	29.196444134565102
23	28.84782987624194	21.43977688687467	18.02335715530765	31.68903608157574
24	27.749694962523968	22.38103538434722	23.339724594735927	26.52954505839289
25	27.31392713962001	20.74254837022834	21.49206902562315	30.4514554645285
26	30.800069722851664	18.94718493986404	20.498518389402125	29.754226947882167
27	28.690953459996514	20.46365696356981	19.400383475684155	31.445006100749524
28	25.62314798675266	19.818720585671954	24.315844518040787	30.2422869095346
29	27.20934286212306	20.63796409273139	26.180930800069724	25.971762245075823
30	26.947882168380687	20.620533379815235	26.947882168380687	25.483702283423394
31	26.233222938818194	22.88652605891581	21.823252571030157	29.056998431235836
32	25.88460868049503	21.1783161931323	26.546975771309043	26.390099355063622
33	25.936900819243508	19.836151298588113	27.575387833362385	26.651560048805994
34	26.564406484225206	22.816803207251176	21.45720759979083	29.161582708732787
35	24.734181628028587	23.09569461390971	24.350705943873105	27.8194178141886
36	21.840683283946312	23.07826390099355	24.769043053860905	30.31200976119923
37	22.67735750392191	22.049851838940214	25.989192957991982	29.283597699145897
38	23.880076695136832	18.45912497821161	28.586369182499567	29.074429144152
39	23.70576956597525	18.668293533205507	29.31845912497821	28.307477775841033
40	22.659926791005752	18.075649294056127	29.12672128290047	30.137702632037648
41	19.104061356109465	20.725117657312182	30.294579048283076	29.87624193829528
42	18.110510719888442	19.400383475684155	32.19452675614433	30.294579048283076
43	18.04078786822381	22.241589681017953	33.2055081052815	26.51211434547673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	5.5
7	10.0
8	6.0
9	2.0
10	7.0
11	12.0
12	12.0
13	15.0
14	18.0
15	35.0
16	52.0
17	42.5
18	33.0
19	33.0
20	38.0
21	43.0
22	33.0
23	23.0
24	21.5
25	20.0
26	20.0
27	19.0
28	18.0
29	20.5
30	23.0
31	24.0
32	25.0
33	25.0
34	32.0
35	39.0
36	42.0
37	45.0
38	55.0
39	65.0
40	65.0
41	89.5
42	114.0
43	160.5
44	207.0
45	311.5
46	416.0
47	416.0
48	570.5
49	725.0
50	684.0
51	643.0
52	504.0
53	365.0
54	365.0
55	361.0
56	357.0
57	347.0
58	337.0
59	326.0
60	315.0
61	315.0
62	335.5
63	356.0
64	356.5
65	357.0
66	339.0
67	321.0
68	321.0
69	303.5
70	286.0
71	247.0
72	208.0
73	171.5
74	135.0
75	135.0
76	106.0
77	77.0
78	66.5
79	56.0
80	38.5
81	21.0
82	21.0
83	14.0
84	7.0
85	5.5
86	4.0
87	2.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.97664284469235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.81246013183075	76.90430538609029
2	3.317031681905167	5.43838242984138
3	0.9993621092919412	2.4577305211783163
4	0.425260472039124	1.3944570332926616
5	0.2338932596215182	0.9586892103887049
6	0.212630236019562	1.045842774969496
7	0.212630236019562	1.2201499041310788
8	0.08505209440782478	0.5577828133170646
9	0.04252604720391239	0.31375283249084884
>10	0.6591537316606422	9.708907094300157
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	45	0.7843820812271222	TruSeq Adapter, Index 6 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	41	0.7146592295624891	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	27	0.4706292487362733	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.4183371099877985	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	23	0.4009063970716402	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	22	0.38347568415548194	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	22	0.38347568415548194	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	21	0.36604497123932367	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.36604497123932367	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	20	0.3486142583231654	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	18	0.31375283249084884	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	17	0.29632211957469057	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	17	0.29632211957469057	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	17	0.29632211957469057	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	16	0.2788914066585323	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	16	0.2788914066585323	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	16	0.2788914066585323	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	15	0.261460693742374	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	15	0.261460693742374	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	15	0.261460693742374	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	14	0.2440299808262158	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	14	0.2440299808262158	RNA PCR Primer, Index 6 (95% over 22bp)
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	13	0.22659926791005752	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	12	0.20916855499389925	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.20916855499389925	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	11	0.19173784207774097	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	11	0.19173784207774097	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	11	0.19173784207774097	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	11	0.19173784207774097	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	10	0.1743071291615827	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	10	0.1743071291615827	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA	9	0.15687641624542442	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	9	0.15687641624542442	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	8	0.13944570332926615	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	8	0.13944570332926615	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	8	0.13944570332926615	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	8	0.13944570332926615	No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT	7	0.1220149904131079	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	7	0.1220149904131079	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	7	0.1220149904131079	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	7	0.1220149904131079	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	7	0.1220149904131079	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	7	0.1220149904131079	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	7	0.1220149904131079	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	7	0.1220149904131079	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	7	0.1220149904131079	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	7	0.1220149904131079	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCTG	6	0.10458427749694962	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	6	0.10458427749694962	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	6	0.10458427749694962	ABI Solid3 Adapter B (100% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	6	0.10458427749694962	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	6	0.10458427749694962	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA	6	0.10458427749694962	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCT	6	0.10458427749694962	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAA	6	0.10458427749694962	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	6	0.10458427749694962	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	6	0.10458427749694962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.017430712916158272	0.0
17	0.0	0.0	0.0	0.034861425832316544	0.0
18	0.0	0.0	0.0	0.034861425832316544	0.0
19	0.0	0.0	0.0	0.034861425832316544	0.0
20	0.0	0.0	0.0	0.034861425832316544	0.0
21	0.0	0.0	0.0	0.034861425832316544	0.0
22	0.0	0.0	0.0	0.05229213874847481	0.0
23	0.0	0.0	0.0	0.08715356458079135	0.0
24	0.0	0.0	0.0	0.1220149904131079	0.0
25	0.0	0.0	0.0	0.1220149904131079	0.0
26	0.0	0.0	0.0	0.13944570332926617	0.0
27	0.0	0.0	0.0	0.19173784207774097	0.0
28	0.0	0.0	0.0	0.4880599616524316	0.0
29	0.0	0.0	0.0	0.8715356458079135	0.0
30	0.0	0.0	0.0	1.4641798849572947	0.0
31	0.0	0.0	0.0	2.71919121492069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATGC	25	0.0050554466	29.6	34
TATGCCC	25	0.0050554466	29.6	36
ATATGCC	25	0.0050554466	29.6	35
CTTATTG	35	7.829244E-4	26.42857	28
ATTCCTC	35	7.829244E-4	26.42857	19
GTATCAA	45	1.1117493E-4	24.666666	2
GGTGATT	40	0.0017067059	23.125	15
CAGTCGG	40	0.0017067059	23.125	10
TTATTGA	40	0.0017067059	23.125	29
TGATTCC	40	0.0017067059	23.125	17
AGTCGGT	40	0.0017067059	23.125	11
GTGATTC	40	0.0017067059	23.125	16
GATTCCT	40	0.0017067059	23.125	18
GGTATCA	50	2.2734379E-4	22.2	1
GGGCAGT	45	0.0033850367	20.555555	7
GCAGTCG	45	0.0033850367	20.555555	9
CGGTGAT	45	0.0033850367	20.555555	14
GGCAGTC	45	0.0033850367	20.555555	8
GTCGGTG	45	0.0033850367	20.555555	12
AACGCAG	55	4.3331872E-4	20.181818	7
>>END_MODULE