FastQCFastQC Report
Fri 10 Feb 2017
ERR1631478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999592
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.16626783727760927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14090.14095751066435105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12590.12595138816637189No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10930.10934461260194159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.025.287771
GTCTAGA1850.024.01
ACTGATC2250.021.3777778
TTAATAC1303.274181E-1119.9230773
GTTCTAG2100.018.51
TCTAGAC2200.018.53
ACGGAGT1900.018.526
GCCTCTA1850.018.025
CGAACGA1900.017.52631616
ATACACA4950.017.19191737
CTAGAAC2300.016.8913043
TAGAACT2750.016.8181824
TAGACAT2800.016.5178595
TAATACT904.4450237E-516.4444454
CTTATAC17750.016.25915337
TCCGATA2050.016.2439048
TAACGAA2050.016.24390413
TGTATAC803.3820144E-416.18753
CTAGACA2300.016.0869564
TATACAC7150.016.04195837
Produced by FastQC (version 0.11.5)