##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631478.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999592
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81631305572674	34.0	31.0	34.0	31.0	34.0
2	32.996609616723624	34.0	33.0	34.0	31.0	34.0
3	33.11902256120497	34.0	33.0	34.0	31.0	34.0
4	36.43903712714788	37.0	37.0	37.0	35.0	37.0
5	36.41651994013557	37.0	37.0	37.0	35.0	37.0
6	36.45512569128204	37.0	37.0	37.0	35.0	37.0
7	36.4512261002489	37.0	37.0	37.0	35.0	37.0
8	36.44113398266493	37.0	37.0	37.0	35.0	37.0
9	38.294018959735574	39.0	39.0	39.0	37.0	39.0
10	38.25496002368966	39.0	39.0	39.0	37.0	39.0
11	38.31656615899287	39.0	39.0	39.0	37.0	39.0
12	38.09821006970844	39.0	39.0	39.0	37.0	39.0
13	38.223356129300754	39.0	39.0	39.0	37.0	39.0
14	39.751951796332904	41.0	40.0	41.0	38.0	41.0
15	39.749120641221616	41.0	40.0	41.0	38.0	41.0
16	39.73485281995054	41.0	40.0	41.0	38.0	41.0
17	39.72618728441204	41.0	40.0	41.0	38.0	41.0
18	39.71367117784056	41.0	40.0	41.0	38.0	41.0
19	39.72019583990268	41.0	40.0	41.0	38.0	41.0
20	39.68482540876678	41.0	40.0	41.0	37.0	41.0
21	39.657309182146314	41.0	40.0	41.0	37.0	41.0
22	39.60690261626744	41.0	40.0	41.0	37.0	41.0
23	39.5561479083466	41.0	40.0	41.0	37.0	41.0
24	39.526647872331914	41.0	39.0	41.0	37.0	41.0
25	39.522527191093964	41.0	39.0	41.0	37.0	41.0
26	39.44886313615955	41.0	39.0	41.0	37.0	41.0
27	39.388049324124246	41.0	39.0	41.0	36.0	41.0
28	39.320129612882056	41.0	39.0	41.0	36.0	41.0
29	39.25686079920607	41.0	39.0	41.0	36.0	41.0
30	39.21326701294128	41.0	39.0	41.0	36.0	41.0
31	39.17281450831939	41.0	39.0	41.0	35.0	41.0
32	39.105654106875605	41.0	39.0	41.0	35.0	41.0
33	39.02415085354825	40.0	39.0	41.0	35.0	41.0
34	38.985038895869515	40.0	39.0	41.0	35.0	41.0
35	38.90377273927763	40.0	39.0	41.0	35.0	41.0
36	38.85894745055983	40.0	39.0	41.0	35.0	41.0
37	38.805792763447485	40.0	38.0	41.0	35.0	41.0
38	38.733735364028526	40.0	38.0	41.0	35.0	41.0
39	38.6626603654291	40.0	38.0	41.0	35.0	41.0
40	38.5834480468031	40.0	38.0	41.0	35.0	41.0
41	38.507004857982054	40.0	38.0	41.0	35.0	41.0
42	38.409180945825895	40.0	38.0	41.0	35.0	41.0
43	37.52353860375033	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	2.0
15	2.0
16	4.0
17	8.0
18	16.0
19	33.0
20	58.0
21	113.0
22	226.0
23	384.0
24	623.0
25	944.0
26	1473.0
27	2036.0
28	2969.0
29	4068.0
30	5546.0
31	7577.0
32	10008.0
33	13643.0
34	20104.0
35	28977.0
36	46793.0
37	87479.0
38	207268.0
39	559234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35889042729434	17.841179201114056	12.01900375353144	26.780926618060167
2	19.434229165499524	19.752659084906643	33.27857765968515	27.534534089908686
3	20.277573249885954	20.856209333408028	28.892588175975796	29.973629240730215
4	15.10086115134975	14.990516130581277	33.18744047571409	36.721182242354885
5	16.68380699325325	34.26888170373512	32.24045410527495	16.806857197736676
6	35.74598436161954	33.546386925865754	15.330554866385485	15.377073846129221
7	30.991944713443086	29.30265548343724	19.884412840438902	19.820986962680774
8	28.595967154599077	31.398510592321667	19.475646063593945	20.52987618948531
9	27.304340170789683	14.016418698829122	19.094190429695317	39.58505070068588
10	18.706432224347534	24.967286652954403	30.846885529295953	25.47939559340211
11	37.97099216480324	20.472752883176337	20.32939439291231	21.226860559108115
12	23.68506350591041	24.29031044666224	27.101757517066964	24.922868530360386
13	31.325180673714875	18.11389046731066	23.979983833404027	26.580945025570436
14	24.083526078640087	19.386609736772602	23.412152158080495	33.117712026506815
15	26.75681678124675	26.018615595162824	20.848506190525733	26.37606143306469
16	26.765120169028968	24.43717036550913	22.604722726872563	26.192986738589347
17	25.569032165123367	24.759401835949067	23.74568824080225	25.925877758125317
18	25.975397962368646	23.171253871579605	24.7557003257329	26.09764784031885
19	26.030420411527906	23.906453833163933	24.29651297729474	25.76661277801343
20	26.34224763703591	23.46357313784024	24.095430935821817	26.098748289302037
21	27.45400123250286	23.63804432208341	23.913256608696347	24.994697836717382
22	27.424289109956863	23.24438370855309	23.52469807681534	25.806629104674705
23	26.348350126851756	23.25188676980208	24.022501180481637	26.37726192286453
24	25.76691290046339	23.82942240434097	23.773899751098448	26.62976494409719
25	26.321839310438662	23.22087411663959	23.901251710697967	26.556034862223786
26	26.03032037071125	23.881343588183977	24.132646119616805	25.955689921487966
27	26.378362371847714	23.271894933132717	23.962376649673068	26.3873660453465
28	25.634258777581252	23.32741758637524	24.68627199897558	26.352051637067923
29	25.17907306180922	23.733683342803864	24.695375713291025	26.391867882095895
30	24.874048611833626	24.314920487558926	24.84553697908747	25.96549392151998
31	26.03462212582734	23.653450607848004	23.89024722086611	26.421680045458544
32	25.41366877686096	23.440463709193352	24.2750042017143	26.870863312231393
33	24.967786857037673	23.17305460627936	25.05862391855877	26.800534618124196
34	25.98990388078336	22.547399338930283	25.167168204627487	26.295528575658867
35	25.28211510296201	22.720169829290352	25.898266492729032	26.09944857501861
36	24.611041304852378	22.73827721710458	25.985302003217313	26.66537947482573
37	25.066927306340986	21.841511336625345	26.241206412216183	26.850354944817482
38	24.085326813339844	21.599212478691307	27.372067803663896	26.943392904304957
39	24.327325548823918	21.310994885913452	27.679893396505772	26.681786168756854
40	23.581521260674354	21.80039456098088	28.190001520620413	26.42808265772435
41	23.076115054942417	21.518079376385565	29.065558747969174	26.340246820702845
42	23.22757685135535	21.721962560724776	28.914397074006192	26.136063513913676
43	21.923744887914268	21.05378994629809	29.389791034742174	27.632674131045466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	147.5
2	141.0
3	275.5
4	410.0
5	410.0
6	501.5
7	593.0
8	543.0
9	493.0
10	746.5
11	1000.0
12	1000.0
13	1448.0
14	1896.0
15	2687.0
16	3478.0
17	3449.5
18	3421.0
19	3421.0
20	4038.5
21	4656.0
22	4648.5
23	4641.0
24	5480.5
25	6320.0
26	6320.0
27	7569.0
28	8818.0
29	10679.0
30	12540.0
31	14580.0
32	16620.0
33	16620.0
34	20597.0
35	24574.0
36	29291.5
37	34009.0
38	37953.5
39	41898.0
40	41898.0
41	46256.5
42	50615.0
43	54549.0
44	58483.0
45	62439.0
46	66395.0
47	66395.0
48	69643.5
49	72892.0
50	74718.0
51	76544.0
52	78164.0
53	79784.0
54	79784.0
55	77861.0
56	75938.0
57	73213.5
58	70489.0
59	68466.0
60	66443.0
61	66443.0
62	62749.0
63	59055.0
64	53675.5
65	48296.0
66	42725.0
67	37154.0
68	37154.0
69	31366.0
70	25578.0
71	21713.0
72	17848.0
73	13946.5
74	10045.0
75	10045.0
76	8093.0
77	6141.0
78	5062.5
79	3984.0
80	3593.5
81	3203.0
82	3203.0
83	2630.0
84	2057.0
85	1731.5
86	1406.0
87	1159.5
88	913.0
89	913.0
90	696.5
91	480.0
92	300.5
93	121.0
94	85.5
95	50.0
96	50.0
97	29.0
98	8.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	999592.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.59451514682721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3580131824699	52.934164529819036
2	6.631090562477433	8.036154350560436
3	2.083747557440213	3.7879101879442554
4	1.0261307878633406	2.4871159027124374
5	0.579001043062169	1.7542143736929676
6	0.415713437718446	1.5113972519141992
7	0.28952894370503546	1.2280706175345757
8	0.21694322301127705	1.0516455530206672
9	0.17264227704034069	0.9415057563993298
>10	1.050816788405301	12.27543090043355
>50	0.10072043063103897	4.1932116469148895
>100	0.07183599745254801	8.056423593854166
>500	0.00315215677120936	1.1975913883382365
>1k	6.636119518335496E-4	0.5451639468612656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1662	0.16626783727760927	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1409	0.14095751066435105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.12595138816637189	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1093	0.10934461260194159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	1.000408166531945E-4	0.0	0.0	0.0
12	0.0	1.000408166531945E-4	0.0	2.00081633306389E-4	0.0
13	0.0	1.000408166531945E-4	0.0	2.00081633306389E-4	0.0
14	0.0	1.000408166531945E-4	0.0	2.00081633306389E-4	0.0
15	0.0	1.000408166531945E-4	0.0	4.00163266612778E-4	1.000408166531945E-4
16	0.0	1.000408166531945E-4	0.0	0.001000408166531945	1.000408166531945E-4
17	0.0	1.000408166531945E-4	0.0	0.0013005306164915284	1.000408166531945E-4
18	0.0	1.000408166531945E-4	0.0	0.001600653066451112	1.000408166531945E-4
19	0.0	1.000408166531945E-4	0.0	0.0017006938831043066	1.000408166531945E-4
20	0.0	1.000408166531945E-4	0.0	0.00200081633306389	1.000408166531945E-4
21	0.0	1.000408166531945E-4	0.0	0.0027011020496362516	1.000408166531945E-4
22	0.0	1.000408166531945E-4	0.0	0.004301755116087364	1.000408166531945E-4
23	0.0	2.00081633306389E-4	0.0	0.0062025306324980595	1.000408166531945E-4
24	0.0	2.00081633306389E-4	0.0	0.0091037143154407	1.000408166531945E-4
25	0.0	2.00081633306389E-4	0.0	0.01220497963168973	1.000408166531945E-4
26	0.0	2.00081633306389E-4	0.0	0.02090853068051765	1.000408166531945E-4
27	0.0	2.00081633306389E-4	0.0	0.07743159208957255	1.000408166531945E-4
28	0.0	2.00081633306389E-4	0.0	0.288017511144547	1.000408166531945E-4
29	0.0	2.00081633306389E-4	0.0	0.5557267365084955	1.000408166531945E-4
30	0.0	2.00081633306389E-4	0.0	0.904769145811491	1.000408166531945E-4
31	0.0	2.00081633306389E-4	0.0	1.658676740109965	1.000408166531945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	695	0.0	25.28777	1
GTCTAGA	185	0.0	24.0	1
ACTGATC	225	0.0	21.377777	8
TTAATAC	130	3.274181E-11	19.923077	3
GTTCTAG	210	0.0	18.5	1
TCTAGAC	220	0.0	18.5	3
ACGGAGT	190	0.0	18.5	26
GCCTCTA	185	0.0	18.0	25
CGAACGA	190	0.0	17.526316	16
ATACACA	495	0.0	17.191917	37
CTAGAAC	230	0.0	16.891304	3
TAGAACT	275	0.0	16.818182	4
TAGACAT	280	0.0	16.517859	5
TAATACT	90	4.4450237E-5	16.444445	4
CTTATAC	1775	0.0	16.259153	37
TCCGATA	205	0.0	16.243904	8
TAACGAA	205	0.0	16.243904	13
TGTATAC	80	3.3820144E-4	16.1875	3
CTAGACA	230	0.0	16.086956	4
TATACAC	715	0.0	16.041958	37
>>END_MODULE