FastQCFastQC Report
Fri 10 Feb 2017
ERR1631477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347242
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3690.10626594709165366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG303.5943516E-430.8333321
CACTAAG358.8568806E-426.428571
GGTATCA3250.022.7692321
ATACACA2550.021.03921737
CGACTTT555.133163E-420.18181835
GCAGTCG3250.018.7846169
CAGTCGG3250.018.78461610
AGTCGGT3250.018.78461611
CGGTGAT3450.017.69565214
TCGGTGA3350.017.67164213
CGCCTTA2450.017.36734625
TCGTATT752.0620358E-417.26666626
CTTATAC11300.017.19026637
GGCAGTC3450.017.159428
CCGCTTA650.00157693317.07692325
CTTATTG3250.017.07692328
GTATCAA4350.017.0114962
TACACAT1107.7690675E-716.81818237
GTGATTC3700.016.516
GGACTCA803.3740688E-416.18756
Produced by FastQC (version 0.11.5)