FastQCFastQC Report
Fri 10 Feb 2017
ERR1631476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153015
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.9698395582132471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13100.8561252164820442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10750.70254550207496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.30127765251772703No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2780.18168153449008267No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG2620.17122504329640884No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC2210.1444302846126197No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG2190.14312322321341045No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA1870.12221024082606281No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT1870.12221024082606281No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1840.12024964872724897No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC1830.11959611802764435No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC1700.11110021893278436No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG1660.10848609613436591No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA1650.1078325654347613No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.10456491193673824No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC1590.10391138123713362No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG1560.10195078913831977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT200.001836953637.01
GTACTGC250.005481648329.5999986
TCACAGA358.834154E-426.428571
TGTTATT400.001923586123.12500210
GGTATCA5850.022.4529911
ACCTGTT450.003810894720.5555557
CTTCTGA450.003810894720.5555551
GCTGTGG450.003810894720.5555551
GCTCAAT450.003810894720.55555517
GGCAAAA555.11514E-420.18181812
GCAGTCG1551.8189894E-1219.0967739
CAGTCGG1551.8189894E-1219.09677310
AGTCGGT1650.019.06060611
ATGGGCG609.187024E-418.55
TCGGTGA1601.8189894E-1218.513
GAATCTT500.00700772418.49999811
GAGCATG500.00700772418.4999989
CTTATAC2350.018.10638437
GTGATTC1750.017.9714316
TGATTCC1800.017.47222117
Produced by FastQC (version 0.11.5)