##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153015
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.757415939613765	34.0	31.0	34.0	31.0	34.0
2	32.91761592000784	34.0	33.0	34.0	31.0	34.0
3	33.0100774433879	34.0	33.0	34.0	31.0	34.0
4	36.37708067836487	37.0	37.0	37.0	35.0	37.0
5	36.35891252491586	37.0	37.0	37.0	35.0	37.0
6	36.41729242231154	37.0	37.0	37.0	35.0	37.0
7	36.397830278077315	37.0	37.0	37.0	35.0	37.0
8	36.406456883312096	37.0	37.0	37.0	35.0	37.0
9	38.25321046956181	39.0	39.0	39.0	37.0	39.0
10	38.20236578113257	39.0	39.0	39.0	37.0	39.0
11	38.273594092082476	39.0	39.0	39.0	37.0	39.0
12	38.03817272816391	39.0	39.0	39.0	37.0	39.0
13	38.17476717968827	39.0	39.0	39.0	37.0	39.0
14	39.65332156978074	41.0	40.0	41.0	37.0	41.0
15	39.62662484070189	41.0	39.0	41.0	37.0	41.0
16	39.60120903179427	41.0	39.0	41.0	37.0	41.0
17	39.59338626932	41.0	39.0	41.0	37.0	41.0
18	39.61523380060778	41.0	40.0	41.0	37.0	41.0
19	39.629611475999084	41.0	40.0	41.0	37.0	41.0
20	39.60020259451688	41.0	40.0	41.0	37.0	41.0
21	39.575904323105576	41.0	40.0	41.0	37.0	41.0
22	39.52689605594223	41.0	39.0	41.0	37.0	41.0
23	39.46929386007908	41.0	39.0	41.0	37.0	41.0
24	39.43416658497533	41.0	39.0	41.0	36.0	41.0
25	39.41103813351632	41.0	39.0	41.0	36.0	41.0
26	39.300160115021406	41.0	39.0	41.0	36.0	41.0
27	39.21582851354442	41.0	39.0	41.0	35.0	41.0
28	39.14154167892037	41.0	39.0	41.0	35.0	41.0
29	39.02624579289612	40.0	39.0	41.0	35.0	41.0
30	38.91208705028919	40.0	38.0	41.0	35.0	41.0
31	38.74620788811554	40.0	38.0	41.0	35.0	41.0
32	38.64414599875829	40.0	38.0	41.0	35.0	41.0
33	38.52868019475215	40.0	38.0	41.0	35.0	41.0
34	38.431807339149756	40.0	38.0	41.0	35.0	41.0
35	38.31390386563409	40.0	38.0	41.0	35.0	41.0
36	38.2102538966768	40.0	37.0	41.0	35.0	41.0
37	38.07775708263895	40.0	37.0	41.0	34.0	41.0
38	37.94646276508839	40.0	37.0	41.0	34.0	41.0
39	37.77512662157305	40.0	37.0	41.0	34.0	41.0
40	37.61248243636245	40.0	36.0	41.0	33.0	41.0
41	37.43506845734078	40.0	36.0	41.0	33.0	41.0
42	37.253713688200506	40.0	35.0	41.0	33.0	41.0
43	36.28593928699801	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	0.0
17	1.0
18	2.0
19	5.0
20	12.0
21	28.0
22	49.0
23	65.0
24	123.0
25	197.0
26	312.0
27	500.0
28	671.0
29	1001.0
30	1266.0
31	1601.0
32	2016.0
33	2536.0
34	3665.0
35	5231.0
36	8391.0
37	18381.0
38	33343.0
39	73614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.842564454465254	16.67810345390975	12.138025683756494	25.341306407868508
2	19.909812763454564	19.82420024180636	32.74646276508839	27.51952422965069
3	20.74371793615005	21.297258438715158	28.420089533705845	29.538934091428942
4	14.145018462242264	16.37094402509558	32.26481063947979	37.219226873182365
5	15.864457732902004	35.863804202202395	31.381237133614352	16.890500931281245
6	34.27833872496161	32.23997647289482	16.989184066921545	16.492500735222038
7	32.215795837009445	29.498415188053457	18.305394895925236	19.980394079011862
8	31.238767441100546	29.518021109041598	18.51452471979871	20.728686730059145
9	28.774956703591155	12.548442963108192	18.41780217625723	40.25879815704343
10	19.529457896284676	25.789628467797275	28.89912753651603	25.78178609940202
11	36.61732509884652	22.29127863281378	18.356370290494397	22.73502597784531
12	23.861712903963664	25.857595660556154	25.518413227461362	24.762278208018824
13	32.94186844427017	18.315851387118908	23.485279220991405	25.257000947619513
14	24.146652288991273	20.007842368395256	24.25971310002287	31.585792242590593
15	28.47629317387184	24.866189589255956	21.6998333496716	24.957683887200602
16	26.905858902721956	23.887854131947847	23.472208606999313	25.73407835833088
17	23.990458451785774	26.22684050583276	24.06953566643793	25.713165375943536
18	23.840799921576316	22.748096591837403	26.684311995555994	26.726791491030287
19	26.383034343038265	25.11061007090808	26.202006339247784	22.30434924680587
20	26.50001633826749	22.86965330196386	26.734633859425543	23.895696500343103
21	26.332712479168705	23.02846126196778	25.69029180145737	24.948534457406137
22	27.37705453713688	22.58602097833546	24.75770349312159	25.27922099140607
23	24.01137143417312	23.821847531287784	25.658922327876354	26.507858706662745
24	24.813907133287586	24.23095774924027	25.485083161781525	25.47005195569062
25	24.76097114661961	23.49573571218508	25.69552004705421	26.047773094141096
26	26.091559651014606	23.64866189589256	24.873378426951607	25.386400026141224
27	25.581805705323006	23.369604287161387	24.253831323726434	26.794758683789173
28	24.332255007678985	24.269516060516942	26.183707479658857	25.214521452145217
29	24.923046760121554	24.376041564552494	26.732673267326735	23.968238407999216
30	24.549880730647324	23.6970231676633	26.736594451524358	25.01650165016502
31	23.71009378165539	24.442701695912167	26.228801097931576	25.61840342450087
32	23.207528673659443	24.894944940038556	27.114335195895826	24.78319119040617
33	23.96758487729961	23.466326830702872	27.522138352449105	25.04394993954841
34	25.043296408848803	23.98719079828775	26.211809299741855	24.75770349312159
35	24.775348822010915	24.442701695912167	25.398163578734113	25.383785903342808
36	23.278109989216745	24.04666209195177	25.861516844753783	26.813711074077705
37	22.797764925007353	23.77348625951704	27.96261804398262	25.46613077149299
38	23.15328562559226	21.93052968663203	28.33447701205764	26.581707675718068
39	22.545502074959973	22.89121981505081	29.206286965330197	25.35699114465902
40	23.099696108224684	21.79198117831585	28.729863085318435	26.378459628141034
41	20.995980786197432	22.610855144920432	30.04411332222331	26.349050746658826
42	20.622814756723198	23.02715420056857	29.786622226579095	26.563408816129137
43	20.824755742901022	22.20239845766755	30.09770283959089	26.875142959840538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	31.5
2	46.0
3	110.0
4	174.0
5	174.0
6	224.5
7	275.0
8	251.5
9	228.0
10	395.5
11	563.0
12	563.0
13	982.0
14	1401.0
15	2081.0
16	2761.0
17	2435.5
18	2110.0
19	2110.0
20	2352.0
21	2594.0
22	1799.5
23	1005.0
24	962.5
25	920.0
26	920.0
27	944.0
28	968.0
29	1152.5
30	1337.0
31	1646.5
32	1956.0
33	1956.0
34	2649.0
35	3342.0
36	3933.5
37	4525.0
38	4960.5
39	5396.0
40	5396.0
41	5900.0
42	6404.0
43	7352.5
44	8301.0
45	8703.0
46	9105.0
47	9105.0
48	10242.5
49	11380.0
50	11826.0
51	12272.0
52	12215.0
53	12158.0
54	12158.0
55	11484.5
56	10811.0
57	9920.0
58	9029.0
59	8993.0
60	8957.0
61	8957.0
62	8764.0
63	8571.0
64	7415.0
65	6259.0
66	5903.5
67	5548.0
68	5548.0
69	5031.0
70	4514.0
71	4466.5
72	4419.0
73	3744.5
74	3070.0
75	3070.0
76	2079.0
77	1088.0
78	860.0
79	632.0
80	549.0
81	466.0
82	466.0
83	334.0
84	202.0
85	158.0
86	114.0
87	89.0
88	64.0
89	64.0
90	45.0
91	26.0
92	15.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	153015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.25004084566873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0356101358181	47.8325654347613
2	7.402028557818545	8.32728817436199
3	2.3968584075937307	4.044701499852955
4	1.2791764938248655	2.8781492010587195
5	0.781912607033728	2.199130804169526
6	0.5332806636381592	1.7998235467111066
7	0.38340439869410137	1.509655916086658
8	0.2718685736194537	1.2234094696598372
9	0.20564417748138164	1.0410744044701499
>10	1.5080573015301322	16.102342907558082
>50	0.1370961183209211	5.377904127046368
>100	0.06157707009329508	5.135444237493056
>500	0.0	0.0
>1k	0.0034854945335827396	2.5285102767702514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1484	0.9698395582132471	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1310	0.8561252164820442	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1075	0.70254550207496	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	461	0.30127765251772703	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	278	0.18168153449008267	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	262	0.17122504329640884	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	221	0.1444302846126197	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	219	0.14312322321341045	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	187	0.12221024082606281	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	187	0.12221024082606281	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	184	0.12024964872724897	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	183	0.11959611802764435	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	170	0.11110021893278436	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	166	0.10848609613436591	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	165	0.1078325654347613	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	160	0.10456491193673824	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	159	0.10391138123713362	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	156	0.10195078913831977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	6.53530699604614E-4	0.0
15	0.0	0.0	0.0	6.53530699604614E-4	0.0
16	0.0	0.0	0.0	0.001307061399209228	0.0
17	0.0	0.0	0.0	0.002614122798418456	0.0
18	0.0	0.0	0.0	0.002614122798418456	0.0
19	0.0	0.0	0.0	0.0032676534980230695	0.0
20	0.0	0.0	0.0	0.003921184197627684	0.0
21	0.0	0.0	0.0	0.003921184197627684	0.0
22	0.0	0.0	0.0	0.006535306996046139	0.0
23	0.0	0.0	0.0	0.010456491193673824	0.0
24	0.0	0.0	0.0	0.01829885958892919	0.0
25	0.0	0.0	0.0	0.0235271051857661	0.0
26	0.0	0.0	0.0	0.028755350782603013	0.0
27	0.0	0.0	0.0	0.07450249975492598	0.0
28	0.0	0.0	0.0	0.2195863150671503	0.0
29	0.0	0.0	0.0	0.4267555468418129	0.0
30	0.0	0.0	0.0	0.6613730679998693	0.0
31	0.0	0.0	0.0	1.2580465967388819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	20	0.0018369536	37.0	1
GTACTGC	25	0.0054816483	29.599998	6
TCACAGA	35	8.834154E-4	26.42857	1
TGTTATT	40	0.0019235861	23.125002	10
GGTATCA	585	0.0	22.452991	1
ACCTGTT	45	0.0038108947	20.555555	7
CTTCTGA	45	0.0038108947	20.555555	1
GCTGTGG	45	0.0038108947	20.555555	1
GCTCAAT	45	0.0038108947	20.555555	17
GGCAAAA	55	5.11514E-4	20.181818	12
GCAGTCG	155	1.8189894E-12	19.096773	9
CAGTCGG	155	1.8189894E-12	19.096773	10
AGTCGGT	165	0.0	19.060606	11
ATGGGCG	60	9.187024E-4	18.5	5
TCGGTGA	160	1.8189894E-12	18.5	13
GAATCTT	50	0.007007724	18.499998	11
GAGCATG	50	0.007007724	18.499998	9
CTTATAC	235	0.0	18.106384	37
GTGATTC	175	0.0	17.97143	16
TGATTCC	180	0.0	17.472221	17
>>END_MODULE