FastQCFastQC Report
Fri 10 Feb 2017
ERR1631475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210209
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37660.17039112590709748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29460.1332905621142616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27370.12383444280608757No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA26630.12048634314673408No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23190.10492220418973953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.023.955131
GTATTAA1800.018.51
GTACTAG500.007036886618.51
CCGCTTA2400.017.72916625
CGCTTAT2400.017.72916626
TCGTTTA5950.015.85714330
TATCCCG700.00259350915.8571425
TAGGACG2055.456968E-1215.3414634
GTATCAA24500.015.1775512
CTTATAC14250.015.059648537
GTATTAT1853.0559022E-1015.01
AAGACGG6600.014.8560615
TTAACGG2006.184564E-1114.835
TATACTG1001.0940891E-414.85
CTCTATG10750.014.81
ACGGACC6150.014.7398388
CGTTTAT6150.014.73983831
TATTAGA3050.014.5573762
TCGGTGA7950.014.42767213
CTTATTG7800.014.2307728
Produced by FastQC (version 0.11.5)