##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2210209
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8099383361483	34.0	31.0	34.0	31.0	34.0
2	32.99291017274837	34.0	33.0	34.0	31.0	34.0
3	33.11434258027182	34.0	33.0	34.0	31.0	34.0
4	36.44720974351294	37.0	37.0	37.0	35.0	37.0
5	36.42320748852258	37.0	37.0	37.0	35.0	37.0
6	36.465555519862605	37.0	37.0	37.0	35.0	37.0
7	36.452691125590384	37.0	37.0	37.0	35.0	37.0
8	36.43829158237977	37.0	37.0	37.0	35.0	37.0
9	38.29393193132414	39.0	39.0	39.0	37.0	39.0
10	38.24280011528322	39.0	39.0	39.0	37.0	39.0
11	38.310547554552535	39.0	39.0	39.0	37.0	39.0
12	38.07893914104956	39.0	39.0	39.0	37.0	39.0
13	38.2122930455898	39.0	39.0	39.0	37.0	39.0
14	39.73965539005587	41.0	40.0	41.0	38.0	41.0
15	39.743403904336645	41.0	40.0	41.0	38.0	41.0
16	39.72927401888238	41.0	40.0	41.0	38.0	41.0
17	39.70973469024875	41.0	40.0	41.0	38.0	41.0
18	39.69331361875732	41.0	40.0	41.0	37.0	41.0
19	39.703279644594694	41.0	40.0	41.0	37.0	41.0
20	39.67981037087443	41.0	40.0	41.0	37.0	41.0
21	39.64970552558604	41.0	40.0	41.0	37.0	41.0
22	39.605276243106424	41.0	40.0	41.0	37.0	41.0
23	39.543049548707835	41.0	39.0	41.0	37.0	41.0
24	39.51291755666546	41.0	39.0	41.0	37.0	41.0
25	39.49316829313427	41.0	39.0	41.0	37.0	41.0
26	39.41954493896279	41.0	39.0	41.0	37.0	41.0
27	39.35121520182028	41.0	39.0	41.0	36.0	41.0
28	39.26372030880338	41.0	39.0	41.0	36.0	41.0
29	39.191186444358884	41.0	39.0	41.0	36.0	41.0
30	39.133846618125254	41.0	39.0	41.0	35.0	41.0
31	39.08151401066596	40.0	39.0	41.0	35.0	41.0
32	39.0077603520753	40.0	39.0	41.0	35.0	41.0
33	38.90824940084852	40.0	39.0	41.0	35.0	41.0
34	38.87110811692469	40.0	38.0	41.0	35.0	41.0
35	38.76779888236814	40.0	38.0	41.0	35.0	41.0
36	38.71837052514038	40.0	38.0	41.0	35.0	41.0
37	38.64481368051619	40.0	38.0	41.0	35.0	41.0
38	38.55714957273271	40.0	38.0	41.0	35.0	41.0
39	38.47299146822767	40.0	38.0	41.0	35.0	41.0
40	38.37381939897992	40.0	38.0	41.0	34.0	41.0
41	38.286809075521816	40.0	37.0	41.0	34.0	41.0
42	38.16800945068996	40.0	37.0	41.0	34.0	41.0
43	37.24667350463237	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	2.0
13	1.0
14	1.0
15	2.0
16	6.0
17	14.0
18	48.0
19	101.0
20	177.0
21	317.0
22	570.0
23	949.0
24	1518.0
25	2216.0
26	3284.0
27	4880.0
28	6657.0
29	9271.0
30	12460.0
31	16767.0
32	22120.0
33	30234.0
34	46088.0
35	68576.0
36	111756.0
37	210076.0
38	485161.0
39	1176951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97362421381869	17.355191296388714	11.401274721078414	27.26990976871418
2	20.02579846521302	19.462684298181756	32.01529810076785	28.496219135837382
3	20.008017341346452	21.127368497730306	28.69208296590956	30.172531195013686
4	14.541837446141972	14.89103519169454	33.725905559157525	36.84122180300596
5	16.470976274189457	34.37150061374286	32.529502866018554	16.62802024604913
6	35.748022019637055	33.92448406462918	15.391621335357877	14.935872580375884
7	31.673203755843904	28.717057979584737	20.02991572290222	19.579822541669138
8	29.765194151322337	30.26225121696636	18.930879387424447	21.041675244286854
9	27.91355930592989	13.25069258156129	18.073946852989923	40.7618012595189
10	18.69429542636013	24.16730725465329	30.609865401869236	26.52853191711734
11	39.171137209196054	20.19881377733961	19.715194354922996	20.91485465854134
12	24.007729585754106	23.823448370719692	26.51495854011996	25.65386350340624
13	31.92598528012509	17.251354962358764	23.260967627948308	27.561692129567838
14	24.26956002803355	18.850615484779944	22.593066990497277	34.286757496689226
15	27.481744939053275	25.490485288947788	19.92449582822258	27.103273943776358
16	27.66281378819831	23.78580487184696	21.833998504213856	26.71738283574087
17	26.306154757310278	24.078808836630383	22.968913799554702	26.646122606504637
18	26.78244455614831	22.295674300484706	24.08057337564004	26.841307767726942
19	26.9124775077832	23.270740459386417	23.58062065623658	26.2361613765938
20	26.93826692407822	22.493800360056447	23.314220510368024	27.25371220549731
21	28.201676854994258	23.04623680384977	22.82272852929293	25.929357811863042
22	28.20398432908381	22.45796664478337	22.681248696390252	26.65680032974257
23	26.755207312973567	22.288797122806034	23.374531548826376	27.58146401539402
24	26.337735481124184	23.260967627948308	23.04424604189016	27.35705084903735
25	26.923788655281015	22.13863032862503	23.350687649900983	27.58689336619297
26	26.719916532780385	23.41588510407839	23.295082048801717	26.569116314339503
27	27.180823171021384	22.493528892516498	23.03546859143185	27.290179345030268
28	26.19431013085188	22.755495068565914	24.070438587482	26.97975621310021
29	25.853437389857703	23.194548569841132	24.212008909564663	26.740005130736506
30	25.554189671655486	23.861001380412443	24.041301071527624	26.543507876404448
31	26.824883981560117	23.433937695484907	22.86028153898568	26.8808967839693
32	26.01106954138726	23.158533876208086	23.256624147309147	27.573772435095506
33	25.438092053737904	22.792414653998787	24.177713510351285	27.59177978191203
34	26.81438723668214	22.449008215965097	23.94836868368557	26.788235863667193
35	26.60974595615166	22.872226110743373	24.579621203243676	25.938406729861295
36	25.43497017702851	23.592565227994275	24.012932713603103	26.959531881374115
37	26.03066044885348	22.37136849954009	24.671151008796	26.92682004281043
38	25.209290162152087	22.18627288188583	25.542109366127818	27.06232758983426
39	25.439992326517537	21.848386283831076	25.506501873804694	27.205119515846693
40	25.2009199130037	21.942449786422912	26.33927379718389	26.5173565033895
41	24.130432913810413	21.87295409619633	27.199463942097786	26.79714904789547
42	24.659839861298185	21.851553405130463	27.354607641177825	26.133999092393523
43	23.605776648271725	20.82631099592844	27.845963888482945	27.721948467316892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	51.0
2	65.0
3	213.0
4	361.0
5	361.0
6	445.5
7	530.0
8	504.0
9	478.0
10	747.5
11	1017.0
12	1017.0
13	1770.5
14	2524.0
15	4613.5
16	6703.0
17	6412.0
18	6121.0
19	6121.0
20	6974.0
21	7827.0
22	6893.5
23	5960.0
24	7150.0
25	8340.0
26	8340.0
27	10376.5
28	12413.0
29	16693.5
30	20974.0
31	25800.0
32	30626.0
33	30626.0
34	38858.0
35	47090.0
36	58520.5
37	69951.0
38	80749.5
39	91548.0
40	91548.0
41	100672.5
42	109797.0
43	117358.5
44	124920.0
45	133894.0
46	142868.0
47	142868.0
48	152351.5
49	161835.0
50	166119.0
51	170403.0
52	171564.0
53	172725.0
54	172725.0
55	170194.0
56	167663.0
57	161926.0
58	156189.0
59	154987.0
60	153785.0
61	153785.0
62	143492.5
63	133200.0
64	122501.0
65	111802.0
66	100349.5
67	88897.0
68	88897.0
69	77575.0
70	66253.0
71	58043.0
72	49833.0
73	39596.5
74	29360.0
75	29360.0
76	24040.5
77	18721.0
78	16041.5
79	13362.0
80	11690.5
81	10019.0
82	10019.0
83	8113.0
84	6207.0
85	5312.0
86	4417.0
87	3815.5
88	3214.0
89	3214.0
90	2438.5
91	1663.0
92	1005.5
93	348.0
94	234.0
95	120.0
96	120.0
97	79.0
98	38.0
99	21.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2210209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.97531193856059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.8444893163621	41.06310018598144
2	8.564527834548946	8.389008446070388
3	2.668447175576273	3.920640984462568
4	1.2909310349080227	2.52895000503157
5	0.7508546514095726	1.8386670386651496
6	0.4982373471250742	1.4640797696934842
7	0.3458405775217793	1.1856355115598762
8	0.26392586309154403	1.0340681178849762
9	0.19486455110056458	0.8589196958325955
>10	1.2810338429025656	12.2014038357118
>50	0.1559190554470707	5.353072091638407
>100	0.12351203614395805	12.156562772490089
>500	0.011858188161832867	3.990854199776081
>1k	0.005558525700859156	4.015037345201571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3766	0.17039112590709748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2946	0.1332905621142616	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2737	0.12383444280608757	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2663	0.12048634314673408	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2319	0.10492220418973953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.524458999126327E-5	0.0
11	0.0	0.0	0.0	4.524458999126327E-5	0.0
12	0.0	0.0	0.0	1.809783599650531E-4	0.0
13	0.0	0.0	0.0	1.809783599650531E-4	0.0
14	0.0	0.0	0.0	2.714675399475796E-4	0.0
15	0.0	0.0	0.0	4.97690489903896E-4	0.0
16	0.0	0.0	0.0	7.239134398602123E-4	0.0
17	0.0	0.0	0.0	0.0010406255697990553	0.0
18	0.0	0.0	0.0	0.0013573376997378982	0.0
19	0.0	0.0	0.0	0.0014930714697116878	0.0
20	0.0	0.0	0.0	0.0017192944196680042	1.357337699737898E-4
21	0.0	0.0	0.0	0.001990761959615584	2.2622294995631634E-4
22	0.0	0.0	0.0	0.0029861429394233757	2.2622294995631634E-4
23	0.0	4.524458999126327E-5	0.0	0.003981523919231168	2.2622294995631634E-4
24	0.0	4.524458999126327E-5	0.0	0.005881796698864225	2.2622294995631634E-4
25	0.0	4.524458999126327E-5	0.0	0.00719388980861086	2.2622294995631634E-4
26	0.0	4.524458999126327E-5	0.0	0.00981807602810413	2.2622294995631634E-4
27	0.0	4.524458999126327E-5	0.0	0.025065502855159853	2.714675399475796E-4
28	0.0	9.048917998252654E-5	0.0	0.083521513123872	2.714675399475796E-4
29	0.0	9.048917998252654E-5	0.0	0.16722400460770903	2.714675399475796E-4
30	0.0	9.048917998252654E-5	0.0	0.2696125117579378	2.714675399475796E-4
31	0.0	9.048917998252654E-5	0.0	0.5513958182235255	2.714675399475796E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1560	0.0	23.95513	1
GTATTAA	180	0.0	18.5	1
GTACTAG	50	0.0070368866	18.5	1
CCGCTTA	240	0.0	17.729166	25
CGCTTAT	240	0.0	17.729166	26
TCGTTTA	595	0.0	15.857143	30
TATCCCG	70	0.002593509	15.857142	5
TAGGACG	205	5.456968E-12	15.341463	4
GTATCAA	2450	0.0	15.177551	2
CTTATAC	1425	0.0	15.0596485	37
GTATTAT	185	3.0559022E-10	15.0	1
AAGACGG	660	0.0	14.856061	5
TTAACGG	200	6.184564E-11	14.8	35
TATACTG	100	1.0940891E-4	14.8	5
CTCTATG	1075	0.0	14.8	1
ACGGACC	615	0.0	14.739838	8
CGTTTAT	615	0.0	14.739838	31
TATTAGA	305	0.0	14.557376	2
TCGGTGA	795	0.0	14.427672	13
CTTATTG	780	0.0	14.23077	28
>>END_MODULE