FastQCFastQC Report
Fri 10 Feb 2017
ERR1631474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375260
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.24862761818472526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6870.1830730693385919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6340.16894952832702662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG250.005491685629.59999831
GTATTAA400.001928776923.1250021
GATAACC502.6974053E-422.1999996
GGTATCA3500.020.6142861
GCCTATA450.003821079920.55555537
ATCATAC450.003821079920.5555551
ATACACA1403.6379788E-1219.8214337
CTAGATA609.22102E-418.53
AGATAAC701.21642224E-418.55
TACGAGC701.21642224E-418.514
TAGATAA701.21642224E-418.54
AACCTCG609.22102E-418.59
CTTCTAT500.00702627118.4999981
TAGGACT500.00702627118.4999984
CTAGGAC500.00702627118.4999983
GCAGTCG1900.017.5263169
AGTCGGT1800.017.47222111
AATGCAC752.0625947E-417.2666665
ACGGACC752.0625947E-417.2666668
CGCATCG650.001577254317.07692321
Produced by FastQC (version 0.11.5)