##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375260
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70491659116346	34.0	31.0	34.0	31.0	34.0
2	32.88729680754677	34.0	31.0	34.0	31.0	34.0
3	33.010659276235145	34.0	33.0	34.0	31.0	34.0
4	36.378129829984545	37.0	37.0	37.0	35.0	37.0
5	36.32847625646219	37.0	37.0	37.0	35.0	37.0
6	36.36181847252571	37.0	37.0	37.0	35.0	37.0
7	36.36012631242339	37.0	37.0	37.0	35.0	37.0
8	36.34806800618238	37.0	37.0	37.0	35.0	37.0
9	38.1840297393807	39.0	39.0	39.0	37.0	39.0
10	38.12168363268134	39.0	38.0	39.0	37.0	39.0
11	38.19282897191281	39.0	38.0	39.0	37.0	39.0
12	37.95793849597612	39.0	38.0	39.0	37.0	39.0
13	38.09753504237062	39.0	38.0	39.0	37.0	39.0
14	39.584157650695516	41.0	39.0	41.0	37.0	41.0
15	39.57114800405053	41.0	39.0	41.0	37.0	41.0
16	39.54339657837233	41.0	39.0	41.0	37.0	41.0
17	39.535463412034325	41.0	39.0	41.0	37.0	41.0
18	39.51603155145766	41.0	39.0	41.0	37.0	41.0
19	39.51988754463572	41.0	39.0	41.0	37.0	41.0
20	39.48997495070085	41.0	39.0	41.0	37.0	41.0
21	39.45702712785802	41.0	39.0	41.0	37.0	41.0
22	39.40244363907691	41.0	39.0	41.0	37.0	41.0
23	39.34654639449981	41.0	39.0	41.0	36.0	41.0
24	39.29961360123647	41.0	39.0	41.0	36.0	41.0
25	39.290321377178486	41.0	39.0	41.0	36.0	41.0
26	39.196162660555345	40.0	39.0	41.0	36.0	41.0
27	39.12768214038267	40.0	39.0	41.0	36.0	41.0
28	39.05021851516282	40.0	39.0	41.0	35.0	41.0
29	38.96899483025103	40.0	39.0	41.0	35.0	41.0
30	38.907053776048606	40.0	39.0	41.0	35.0	41.0
31	38.850692852955284	40.0	38.0	41.0	35.0	41.0
32	38.77610190268081	40.0	38.0	41.0	35.0	41.0
33	38.68060011725204	40.0	38.0	41.0	35.0	41.0
34	38.63064008953792	40.0	38.0	41.0	35.0	41.0
35	38.52920908170335	40.0	38.0	41.0	35.0	41.0
36	38.470271278580185	40.0	38.0	41.0	35.0	41.0
37	38.393409902467624	40.0	38.0	41.0	34.0	41.0
38	38.3085993711027	40.0	38.0	41.0	34.0	41.0
39	38.226989287427386	40.0	38.0	41.0	34.0	41.0
40	38.13410435431434	40.0	37.0	41.0	34.0	41.0
41	38.03549805468209	40.0	37.0	41.0	34.0	41.0
42	37.92135585993711	40.0	37.0	41.0	33.0	41.0
43	36.95622768214038	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	6.0
17	5.0
18	7.0
19	11.0
20	34.0
21	69.0
22	101.0
23	220.0
24	305.0
25	458.0
26	693.0
27	1031.0
28	1376.0
29	1942.0
30	2643.0
31	3360.0
32	4566.0
33	6140.0
34	8940.0
35	13146.0
36	21141.0
37	38957.0
38	89774.0
39	180333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.248147950754145	17.360763204178436	12.243511165591856	27.147577679475564
2	19.715663806427543	19.105153760059693	33.203645472472424	27.975536961040344
3	20.427703458935138	20.579331663380056	28.306773970047434	30.68619090763737
4	15.363747801524278	15.091403293716358	32.33811224217876	37.20673666258061
5	17.024196557053774	33.98044022810851	31.89921654319672	17.096146671640998
6	36.0853808026435	32.99578958588712	15.345627031924533	15.573202579544848
7	31.74359111016362	28.64600543623088	19.58029099824122	20.03011245536428
8	29.24558972445771	31.3654532857219	18.67665085540692	20.712306134413474
9	27.939828385652614	13.872515056227682	18.3432819911528	39.8443745669669
10	19.255716036881097	24.69940841016895	30.098065341363323	25.946810211586634
11	38.47892128124501	20.43116772371156	19.641315354687418	21.448595640356018
12	24.04332995789586	24.00522304535522	26.545062090284073	25.40638490646485
13	32.17742365293397	17.690934285562008	23.19032137717849	26.941320684325536
14	24.262111602622184	19.014283430155093	22.906784629323667	33.81682033789906
15	27.40153493577786	25.47193945531098	20.24436390769067	26.882161701220486
16	27.14437989660502	23.97830837286148	22.281085114320735	26.59622661621276
17	25.794915525235833	24.602142514523266	23.273996695624366	26.328945264616532
18	26.30496189308746	22.843361935724566	24.315940947609658	26.53573522357832
19	26.631668709694612	23.910355486862443	23.82881202366359	25.62916377977935
20	26.959441453925276	22.94675691520546	23.529286361456055	26.564515269413207
21	28.352608857858552	23.105313649203218	22.923040025582264	25.619037467355966
22	27.990459947769548	22.72477748760859	22.955284336193575	26.32947822842829
23	26.768373927410327	22.654159782550764	23.480520172680276	27.096946117358634
24	26.296434472099346	23.30730693385919	23.477322389809732	26.91893620423173
25	26.72094014816394	22.46096040078879	23.472525715503917	27.345573735543354
26	26.560518040825027	23.412034322869477	23.69716996215957	26.330277674145925
27	27.084421467782338	22.745829558173	23.34727921974098	26.822469754303686
28	26.14214144859564	23.157810584661302	24.02947289879017	26.670575067952885
29	25.714171507754624	23.541278047220594	24.30181740659809	26.44273303842669
30	25.363214837712516	23.898630283003783	24.323402440974256	26.414752438309442
31	26.32734637318126	23.43974844108085	23.325694185364814	26.907211000373078
32	25.417310664605875	23.024569631722006	24.12034322869477	27.437776474977348
33	25.466076853381654	22.64536587965677	24.68768320631029	27.20087406065128
34	26.465650482332247	22.3338485316847	24.592549165911635	26.607951820071417
35	25.708308905825294	22.404199754836647	25.360816500559615	26.526674838778447
36	24.716729734051057	22.618717689068912	25.479400948675586	27.185151628204444
37	25.21238607898524	21.521611682566753	25.96626339071577	27.29973884773224
38	24.32446836859777	21.103768054149125	27.10094334594681	27.47082023130629
39	24.496349197889465	20.56893886905079	27.670681660715235	27.26403027234451
40	24.03373660928423	21.088578585514043	28.12076959974418	26.75691520545755
41	22.86547993391249	21.02275755476203	29.34791877631509	26.763843735010397
42	22.80125779459575	21.406757981133083	29.189895006129085	26.60208921814209
43	21.757448169269306	20.805308319565103	29.522464424665568	27.914779086500026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	29.5
2	30.0
3	73.5
4	117.0
5	117.0
6	147.0
7	177.0
8	171.0
9	165.0
10	263.0
11	361.0
12	361.0
13	555.0
14	749.0
15	1277.0
16	1805.0
17	1674.5
18	1544.0
19	1544.0
20	1771.0
21	1998.0
22	1763.5
23	1529.0
24	1766.5
25	2004.0
26	2004.0
27	2415.0
28	2826.0
29	3485.5
30	4145.0
31	5085.5
32	6026.0
33	6026.0
34	7157.0
35	8288.0
36	10022.0
37	11756.0
38	13364.0
39	14972.0
40	14972.0
41	16235.5
42	17499.0
43	18887.5
44	20276.0
45	21812.5
46	23349.0
47	23349.0
48	25169.5
49	26990.0
50	27670.0
51	28350.0
52	28699.0
53	29048.0
54	29048.0
55	28518.5
56	27989.0
57	27573.5
58	27158.0
59	26331.5
60	25505.0
61	25505.0
62	23906.5
63	22308.0
64	20674.0
65	19040.0
66	16937.0
67	14834.0
68	14834.0
69	13089.5
70	11345.0
71	9907.0
72	8469.0
73	6788.0
74	5107.0
75	5107.0
76	4141.0
77	3175.0
78	2730.5
79	2286.0
80	1969.0
81	1652.0
82	1652.0
83	1323.5
84	995.0
85	824.0
86	653.0
87	542.0
88	431.0
89	431.0
90	323.5
91	216.0
92	129.0
93	42.0
94	29.5
95	17.0
96	17.0
97	9.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	375260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.20533247372957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.12418903144466	69.28230264982507
2	4.638236316037364	6.976402084786332
3	1.157508855258267	2.6115251490295233
4	0.5601356313186385	1.6850074553480248
5	0.3148379175578675	1.1838745132638042
6	0.21051607922051213	0.9499159037306766
7	0.139013698712849	0.7318200001071876
8	0.11236141293565599	0.6760141933635222
9	0.08812835038381403	0.5964949701878461
>10	0.5572811611763437	8.026009245839246
>50	0.06898749060392591	3.525854998571895
>100	0.027737238485334895	3.1519817208312317
>500	0.001066816864820573	0.6027971151156963
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.24862761818472526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	687	0.1830730693385919	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.16894952832702662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	2.6648190587859083E-4
14	0.0	0.0	0.0	0.0	2.6648190587859083E-4
15	0.0	0.0	0.0	0.0	2.6648190587859083E-4
16	0.0	0.0	0.0	0.0	5.329638117571817E-4
17	0.0	0.0	0.0	0.0	5.329638117571817E-4
18	0.0	0.0	0.0	0.0	5.329638117571817E-4
19	0.0	0.0	0.0	2.6648190587859083E-4	5.329638117571817E-4
20	0.0	0.0	0.0	7.994457176357725E-4	5.329638117571817E-4
21	0.0	0.0	0.0	7.994457176357725E-4	7.994457176357725E-4
22	0.0	0.0	0.0	0.0013324095293929543	7.994457176357725E-4
23	0.0	0.0	0.0	0.003730746682300272	7.994457176357725E-4
24	0.0	0.0	0.0	0.00639556574108618	7.994457176357725E-4
25	0.0	0.0	0.0	0.009060384799872088	7.994457176357725E-4
26	0.0	0.0	0.0	0.01598891435271545	7.994457176357725E-4
27	0.0	0.0	0.0	0.05596120023450408	7.994457176357725E-4
28	0.0	0.0	0.0	0.24063316100836754	7.994457176357725E-4
29	0.0	2.6648190587859083E-4	0.0	0.4855300325107925	7.994457176357725E-4
30	0.0	2.6648190587859083E-4	0.0	0.810904439588552	7.994457176357725E-4
31	0.0	2.6648190587859083E-4	0.0	1.7020199328465597	7.994457176357725E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	25	0.0054916856	29.599998	31
GTATTAA	40	0.0019287769	23.125002	1
GATAACC	50	2.6974053E-4	22.199999	6
GGTATCA	350	0.0	20.614286	1
GCCTATA	45	0.0038210799	20.555555	37
ATCATAC	45	0.0038210799	20.555555	1
ATACACA	140	3.6379788E-12	19.82143	37
CTAGATA	60	9.22102E-4	18.5	3
AGATAAC	70	1.21642224E-4	18.5	5
TACGAGC	70	1.21642224E-4	18.5	14
TAGATAA	70	1.21642224E-4	18.5	4
AACCTCG	60	9.22102E-4	18.5	9
CTTCTAT	50	0.007026271	18.499998	1
TAGGACT	50	0.007026271	18.499998	4
CTAGGAC	50	0.007026271	18.499998	3
GCAGTCG	190	0.0	17.526316	9
AGTCGGT	180	0.0	17.472221	11
AATGCAC	75	2.0625947E-4	17.266666	5
ACGGACC	75	2.0625947E-4	17.266666	8
CGCATCG	65	0.0015772543	17.076923	21
>>END_MODULE