FastQCFastQC Report
Fri 10 Feb 2017
ERR1631473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192388
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG4470.2323429735742354No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC3130.16269205979582926No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA2980.15489531571615694No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC2810.14605900575919495No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG2670.13878204461816745No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG2650.1377424787408778No Hit
TCCATGGCCAGCGAGAACAGCGAGTGCTCTGTCAAGACGCTCT2490.12942595172256066No Hit
TCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCT2290.11903029294966423No Hit
CCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCCCTCTCTGT2050.10655550242218849No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2050.10655550242218849No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1970.10239723891302992No Hit
ACGCTGGACGCTCCAAGCAGTGAGACTGCCCACCCACTCCCAC1930.10031810715845063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGG303.5884438E-430.8333321
GATAACG250.00548511329.611
ATAACGA250.00548511329.612
GGCACTA1000.027.75000212
GTCGGGA405.9148413E-527.752
ACTATTC1050.026.4285715
CACTATT1050.026.4285714
GGTATCA2100.026.428571
GCACTAT1050.026.4285713
ATACACA809.658834E-1025.437537
GGGTAAT451.3176241E-424.66666811
AGTCGGT451.3176241E-424.66666811
TATTCAT1150.024.13043617
CTATTCA1150.024.13043616
GGGCACT1150.024.13043611
TTCCTCC701.9056642E-723.78571520
CGGGAGT400.001925378323.1254
TTACACT1200.023.1249984
GCAGTCG502.690784E-422.29
GTAATTT502.690784E-422.213
Produced by FastQC (version 0.11.5)