##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192388
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4025406990041	34.0	31.0	34.0	31.0	34.0
2	32.60151360791733	34.0	31.0	34.0	31.0	34.0
3	32.717445994552676	34.0	31.0	34.0	31.0	34.0
4	36.204846456119924	37.0	37.0	37.0	35.0	37.0
5	36.06407364284675	37.0	35.0	37.0	35.0	37.0
6	36.10642035885814	37.0	35.0	37.0	35.0	37.0
7	36.10109258373703	37.0	35.0	37.0	35.0	37.0
8	36.0861072416159	37.0	35.0	37.0	35.0	37.0
9	37.86740857018109	39.0	38.0	39.0	35.0	39.0
10	37.754548100713144	39.0	37.0	39.0	35.0	39.0
11	37.8557082562322	39.0	38.0	39.0	35.0	39.0
12	37.613463417676776	39.0	37.0	39.0	35.0	39.0
13	37.75820217477182	39.0	37.0	39.0	35.0	39.0
14	39.11977358255193	40.0	38.0	41.0	36.0	41.0
15	39.100853483585254	40.0	38.0	41.0	36.0	41.0
16	39.1010665945901	40.0	38.0	41.0	36.0	41.0
17	39.0817722519076	40.0	38.0	41.0	36.0	41.0
18	39.06026363390648	40.0	38.0	41.0	36.0	41.0
19	39.064650601908646	40.0	39.0	41.0	36.0	41.0
20	39.038671850635176	40.0	39.0	41.0	36.0	41.0
21	39.00147098571637	40.0	38.0	41.0	36.0	41.0
22	38.96737842277065	40.0	38.0	41.0	36.0	41.0
23	38.921611535022976	40.0	38.0	41.0	36.0	41.0
24	38.88123999417843	40.0	38.0	41.0	35.0	41.0
25	38.84847287772626	40.0	38.0	41.0	35.0	41.0
26	38.7867070711271	40.0	38.0	41.0	35.0	41.0
27	38.72168222550263	40.0	38.0	41.0	35.0	41.0
28	38.667240160508975	40.0	38.0	41.0	35.0	41.0
29	38.60157598186997	40.0	38.0	41.0	35.0	41.0
30	38.54889078320893	40.0	38.0	41.0	35.0	41.0
31	38.482738008607605	40.0	38.0	41.0	35.0	41.0
32	38.40274861217955	40.0	38.0	41.0	34.0	41.0
33	38.363728506975484	40.0	38.0	41.0	34.0	41.0
34	38.30248248331497	40.0	38.0	41.0	34.0	41.0
35	38.24180822088696	40.0	38.0	41.0	34.0	41.0
36	38.187600058215686	40.0	38.0	41.0	34.0	41.0
37	38.134332702663365	40.0	38.0	41.0	34.0	41.0
38	38.06136037590702	40.0	38.0	41.0	34.0	41.0
39	38.011674324801966	40.0	37.0	41.0	33.0	41.0
40	37.956385013618316	40.0	37.0	41.0	33.0	41.0
41	37.8804083414766	40.0	37.0	41.0	33.0	41.0
42	37.79036114518577	40.0	37.0	41.0	33.0	41.0
43	36.785506372538826	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	6.0
20	12.0
21	32.0
22	57.0
23	68.0
24	168.0
25	246.0
26	359.0
27	591.0
28	788.0
29	1089.0
30	1527.0
31	2042.0
32	2813.0
33	3869.0
34	5597.0
35	8242.0
36	13991.0
37	27313.0
38	61009.0
39	62561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7247229556937	16.031145393683598	11.161818824458907	28.082312826163797
2	21.123458843586917	18.470486724743747	31.63710834355573	28.768946088113605
3	21.714971827764725	19.354117720439945	26.738673929766932	32.1922365220284
4	16.122627190885087	14.347048672474374	30.640164667234966	38.890159469405575
5	17.40129321995135	33.54523151132087	31.59448614258686	17.45898912614092
6	38.55022142753186	31.205688504480527	14.30234733975092	15.941742728236688
7	33.599288936939935	26.76466307669917	18.295319874420443	21.340728111940454
8	30.019543838493046	30.773748882466684	17.860261554774727	21.346445724265546
9	28.871343327026633	13.07462003867185	16.898663118281807	41.15537351601971
10	17.912759631577853	24.822754017922115	30.447844979936377	26.816641370563655
11	39.94479905191592	19.534482400149695	18.466848244173235	22.05387030376115
12	25.76667983450111	22.338711354138514	25.89090795683722	26.00370085452315
13	33.58213609996466	16.692829074578455	22.60120173815415	27.12383308730274
14	24.99324282179762	17.47250348254569	21.392706405804937	36.14154728985176
15	27.407114788864174	25.188161423789424	18.96376073351768	28.44096305382872
16	27.678441482836767	23.36580244090068	20.538183254672848	28.417572821589705
17	27.714826288541904	23.156329916626817	21.76643033869056	27.362413456140715
18	28.591700106035717	21.654157223943283	22.154708193858244	27.599434476162756
19	27.836455496184794	22.7015198453126	22.148990581533152	27.313034076969455
20	27.59787512734682	21.972264382393913	22.28673306027403	28.14312742998524
21	28.17119570867206	21.669230929163984	23.063808553548036	27.095764808615925
22	29.30224338316319	21.556438031478052	21.42025490155311	27.721063683805642
23	27.813065263945774	21.647919828679544	22.029440505644843	28.509574401729836
24	28.102584360770944	21.786182090359066	21.624529596440524	28.486703952429465
25	27.460652431544585	22.322598083040525	22.0185250639333	28.198224421481587
26	28.116618500114356	21.80385471027299	21.7575940287336	28.321932760879058
27	28.394182589350685	22.06582531134998	21.96134894068237	27.578643158616963
28	27.38008607605464	22.1645840696925	22.31064307545169	28.14468677880117
29	27.223631411522547	21.585026093103522	22.544545397841862	28.646797097532072
30	26.757905898496787	22.114165124643947	23.543048422978565	27.584880553880698
31	27.24130403143647	21.821527330186914	22.49776493336383	28.43940370501279
32	26.556230118302597	21.197787803813128	23.916252572925547	28.329729504958728
33	27.515749423040937	21.484188203006426	22.515957336216395	28.484105037736242
34	27.181528993492314	21.313699399130922	23.747842900804624	27.756928706572136
35	25.904942096180633	20.78611971640643	24.218246460278188	29.09069172713475
36	25.39451525043142	20.20760130569474	25.960039087676982	28.43784435619685
37	26.621722768571843	19.30213942657546	25.996423893382126	28.07971391147057
38	25.060814603821445	18.338981641266606	27.652972118843174	28.947231636068775
39	25.731854377611906	18.941410067155957	28.022018005280998	27.304717549951143
40	24.05035657109591	19.11865604923384	27.923259246938482	28.90772813273177
41	23.126182506185415	19.137888017963697	29.966525978751275	27.76940349709961
42	22.77740815435474	19.018337942075387	29.368255816371086	28.83599808719878
43	21.185313013285654	19.370750774476576	29.83554067821278	29.60839553402499
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	5.0
2	2.0
3	13.5
4	25.0
5	25.0
6	25.0
7	25.0
8	28.0
9	31.0
10	45.5
11	60.0
12	60.0
13	91.5
14	123.0
15	181.5
16	240.0
17	218.0
18	196.0
19	196.0
20	236.0
21	276.0
22	236.0
23	196.0
24	223.5
25	251.0
26	251.0
27	306.0
28	361.0
29	516.0
30	671.0
31	852.5
32	1034.0
33	1034.0
34	1408.0
35	1782.0
36	2442.0
37	3102.0
38	3974.0
39	4846.0
40	4846.0
41	5805.0
42	6764.0
43	7417.0
44	8070.0
45	9564.0
46	11058.0
47	11058.0
48	12772.0
49	14486.0
50	15373.0
51	16260.0
52	17411.0
53	18562.0
54	18562.0
55	18951.0
56	19340.0
57	18936.0
58	18532.0
59	17962.5
60	17393.0
61	17393.0
62	16696.0
63	15999.0
64	14055.5
65	12112.0
66	10295.5
67	8479.0
68	8479.0
69	6962.0
70	5445.0
71	4449.0
72	3453.0
73	2393.0
74	1333.0
75	1333.0
76	972.0
77	611.0
78	509.5
79	408.0
80	365.5
81	323.0
82	323.0
83	272.5
84	222.0
85	189.5
86	157.0
87	121.5
88	86.0
89	86.0
90	62.5
91	39.0
92	29.0
93	19.0
94	12.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	192388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63771129176456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17532487931558	40.2935734037466
2	8.114391027990408	8.05559598311745
3	3.0796778956407005	4.586044867663263
4	1.7801606333183242	3.5345239827847887
5	1.0680963799909946	2.650892987088592
6	0.7968836717383793	2.373328897852257
7	0.578028629171597	2.008441274923592
8	0.4272385519963978	1.6965715117366986
9	0.3298532938207483	1.4735846310580702
>10	2.413688388116904	22.66877352017797
>50	0.1759217567043991	5.8142919516809775
>100	0.06073489219556636	4.84437698816974
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	447	0.2323429735742354	No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC	313	0.16269205979582926	No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA	298	0.15489531571615694	No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC	281	0.14605900575919495	No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG	267	0.13878204461816745	No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG	265	0.1377424787408778	No Hit
TCCATGGCCAGCGAGAACAGCGAGTGCTCTGTCAAGACGCTCT	249	0.12942595172256066	No Hit
TCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCT	229	0.11903029294966423	No Hit
CCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCCCTCTCTGT	205	0.10655550242218849	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	205	0.10655550242218849	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	197	0.10239723891302992	No Hit
ACGCTGGACGCTCCAAGCAGTGAGACTGCCCACCCACTCCCAC	193	0.10031810715845063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	5.197829386448219E-4	0.0
17	0.0	0.0	0.0	5.197829386448219E-4	0.0
18	0.0	0.0	0.0	5.197829386448219E-4	0.0
19	0.0	0.0	0.0	5.197829386448219E-4	0.0
20	0.0	0.0	0.0	0.0015593488159344657	0.0
21	0.0	0.0	0.0	0.0020791317545792876	0.0
22	0.0	0.0	0.0	0.0020791317545792876	0.0
23	0.0	0.0	0.0	0.0020791317545792876	0.0
24	0.0	0.0	0.0	0.007276961141027507	0.0
25	0.0	0.0	0.0	0.012474790527475726	0.0
26	0.0	0.0	0.0	0.022870449300372166	0.0
27	0.0	0.0	0.0	0.10031810715845063	0.0
28	0.0	0.0	0.0	0.39451525043141983	0.0
29	0.0	0.0	0.0	0.7848722373536811	0.0
30	0.0	0.0	0.0	1.2890616878391583	0.0
31	0.0	0.0	0.0	2.5594111898871033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGG	30	3.5884438E-4	30.833332	1
GATAACG	25	0.005485113	29.6	11
ATAACGA	25	0.005485113	29.6	12
GGCACTA	100	0.0	27.750002	12
GTCGGGA	40	5.9148413E-5	27.75	2
ACTATTC	105	0.0	26.42857	15
CACTATT	105	0.0	26.42857	14
GGTATCA	210	0.0	26.42857	1
GCACTAT	105	0.0	26.42857	13
ATACACA	80	9.658834E-10	25.4375	37
GGGTAAT	45	1.3176241E-4	24.666668	11
AGTCGGT	45	1.3176241E-4	24.666668	11
TATTCAT	115	0.0	24.130436	17
CTATTCA	115	0.0	24.130436	16
GGGCACT	115	0.0	24.130436	11
TTCCTCC	70	1.9056642E-7	23.785715	20
CGGGAGT	40	0.0019253783	23.125	4
TTACACT	120	0.0	23.124998	4
GCAGTCG	50	2.690784E-4	22.2	9
GTAATTT	50	2.690784E-4	22.2	13
>>END_MODULE