FastQCFastQC Report
Fri 10 Feb 2017
ERR1631472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222981
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15120.12363233770598236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.11382024741185676No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12860.10515290098537916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC405.940435E-527.7500023
TTTAGAC502.7022394E-422.23
GAATCTA2000.021.27500213
TCTAGAC2000.021.2750023
AATCTAC2000.021.27500214
ATTAGAC450.003825945820.5555573
CGACTTG450.003825945820.5555576
GGTATCA11150.020.4080731
TATACAC9450.019.96825437
CTAGACA2150.019.7906974
ATACACT851.245442E-619.5882344
ATACACA5500.019.50909237
GCAGTCG2400.019.2708329
TAGAACT2650.018.8490564
GTGCTTA609.237278E-418.51
TATTAGA701.2192788E-418.52
GGACCTA609.237278E-418.56
AATAACG1800.018.52
CGCCAGT609.237278E-418.523
AAGACGG3300.018.4999985
Produced by FastQC (version 0.11.5)