##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631472.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222981
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.834722698063175	34.0	31.0	34.0	31.0	34.0
2	33.00535576595221	34.0	33.0	34.0	31.0	34.0
3	33.122188325084366	34.0	33.0	34.0	31.0	34.0
4	36.44580496344587	37.0	37.0	37.0	35.0	37.0
5	36.425910132700345	37.0	37.0	37.0	35.0	37.0
6	36.47250693183295	37.0	37.0	37.0	35.0	37.0
7	36.46275780245155	37.0	37.0	37.0	35.0	37.0
8	36.44505679156095	37.0	37.0	37.0	35.0	37.0
9	38.30346505791995	39.0	39.0	39.0	37.0	39.0
10	38.25818716725771	39.0	39.0	39.0	37.0	39.0
11	38.32464118412306	39.0	39.0	39.0	37.0	39.0
12	38.109065471990164	39.0	39.0	39.0	37.0	39.0
13	38.22976808306915	39.0	39.0	39.0	37.0	39.0
14	39.7565481393415	41.0	40.0	41.0	38.0	41.0
15	39.75951466130709	41.0	40.0	41.0	38.0	41.0
16	39.75991532166076	41.0	40.0	41.0	38.0	41.0
17	39.74831579558472	41.0	40.0	41.0	38.0	41.0
18	39.74153482351729	41.0	40.0	41.0	38.0	41.0
19	39.749832581209354	41.0	40.0	41.0	38.0	41.0
20	39.72401697164551	41.0	40.0	41.0	38.0	41.0
21	39.69448012683762	41.0	40.0	41.0	37.0	41.0
22	39.65890148743112	41.0	40.0	41.0	37.0	41.0
23	39.60858754142542	41.0	40.0	41.0	37.0	41.0
24	39.579152905891426	41.0	40.0	41.0	37.0	41.0
25	39.555075671658024	41.0	39.0	41.0	37.0	41.0
26	39.50425476765379	41.0	39.0	41.0	37.0	41.0
27	39.43610980056109	41.0	39.0	41.0	37.0	41.0
28	39.370520065315816	41.0	39.0	41.0	36.0	41.0
29	39.32052501224467	41.0	39.0	41.0	36.0	41.0
30	39.27053159452191	41.0	39.0	41.0	36.0	41.0
31	39.22797819426467	41.0	39.0	41.0	36.0	41.0
32	39.14560487857129	41.0	39.0	41.0	35.0	41.0
33	39.07707642228293	40.0	39.0	41.0	35.0	41.0
34	39.033695535744215	40.0	39.0	41.0	35.0	41.0
35	38.96326353393879	40.0	39.0	41.0	35.0	41.0
36	38.92102902661611	40.0	39.0	41.0	35.0	41.0
37	38.86268552005305	40.0	38.0	41.0	35.0	41.0
38	38.781144596686296	40.0	38.0	41.0	35.0	41.0
39	38.722505909740214	40.0	38.0	41.0	35.0	41.0
40	38.65556210603435	40.0	38.0	41.0	35.0	41.0
41	38.5750588112162	40.0	38.0	41.0	35.0	41.0
42	38.47656750186634	40.0	38.0	41.0	35.0	41.0
43	37.53381205431646	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	0.0
16	4.0
17	6.0
18	24.0
19	36.0
20	75.0
21	144.0
22	210.0
23	408.0
24	666.0
25	1010.0
26	1622.0
27	2346.0
28	3384.0
29	4560.0
30	6460.0
31	8668.0
32	11488.0
33	15432.0
34	22642.0
35	34038.0
36	56220.0
37	106146.0
38	265485.0
39	681902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58646536618312	16.478342672535387	11.684645959340333	27.250546001941157
2	20.262375294464917	19.031775636743333	33.12046548556356	27.585383583228197
3	20.471291050310676	19.992869881052936	28.150232914493355	31.38560615414303
4	15.308169137541794	14.5728347374162	32.21963382914371	37.89936229589831
5	17.023976660307888	33.66004868432134	31.92061037743023	17.39536427794054
6	36.84472612411804	32.31677352305555	15.223866928431432	15.614633424394983
7	32.41039721794533	28.33339193331704	19.09228352689044	20.16392732184719
8	28.998896957516102	31.56238731427553	18.710593214448956	20.728122513759413
9	28.264707301258156	13.828260618930303	17.869778843661514	40.03725323615003
10	18.629807004360657	24.558599029747803	30.317396590789226	26.494197375102313
11	38.47157069488406	20.416997484016512	19.515593455662845	21.595838365436585
12	25.110692643630607	23.376814521239496	26.3667219686978	25.145770866432105
13	32.13688520099658	17.415560830462613	23.64026914563677	26.807284822904037
14	24.800139985821527	18.43037626913255	22.46379951937111	34.3056842256748
15	27.164771979286677	25.250024325807185	20.127622587758925	27.45758110714721
16	27.425446511433947	23.64264040079118	21.611128872811598	27.320784214963272
17	26.246687397432993	24.461704638093316	22.66077723202568	26.630830732448008
18	27.145474868374897	22.454396266172573	23.755970043688333	26.644158821764197
19	27.016200578749793	23.234948049070265	23.243697162915858	26.505154209264088
20	27.1897928095367	22.759961111415468	23.412955720489524	26.637290358558307
21	28.50657532700835	22.480725375128475	23.296273613408548	25.716425684454624
22	28.234616891022835	22.29323268309156	22.254638461268	27.217511964617604
23	26.671469139749515	22.555133726525597	23.270353341548233	27.50304379217666
24	26.553396986543536	22.88784535491557	23.106327898798103	27.45242975974279
25	27.132065011639593	22.689232293878643	23.098069389467213	27.08063330501455
26	27.263219951904404	22.856201363717016	22.92038878772442	26.960189896654157
27	27.313425147242683	22.374100660598977	23.138707796768713	27.173766395389627
28	26.182091136329998	22.823412628650814	23.669051277166204	27.325444957852984
29	26.236630004881516	22.841401460856712	23.441901386857193	27.48006714740458
30	25.66532104750605	23.05743098216571	24.50160713862276	26.775640831705484
31	27.048335174463055	22.59724394737122	23.116221756511344	27.238199121654382
32	25.957476036013645	22.20852163688561	24.011820298107654	27.82218202899309
33	26.187978390506476	22.151366210922326	23.849103134063405	27.81155226450779
34	26.591418836433274	21.70507963737785	24.292119010843177	27.4113825153457
35	25.667119930726646	21.561741351664498	25.17880490375566	27.592333813853198
36	25.299411846954285	21.280788499576037	25.798356638410574	27.621443015059103
37	25.93858776219745	20.539566845273967	26.044476569954888	27.477368822573695
38	24.645436028850817	20.069894789861824	27.37687666447803	27.907792516809337
39	25.261717066741017	19.928600689626414	27.648344495948834	27.161337747683735
40	24.33136737201968	20.90841967291397	27.25545204708822	27.504760907978127
41	23.575018745180834	20.56058107198722	28.843702396030686	27.020697786801268
42	23.620563197629398	20.47317170095038	28.544024804964263	27.36224029645595
43	21.857739408870618	20.631964028876983	28.598400138677544	28.911896423574856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	48.5
2	46.0
3	127.5
4	209.0
5	209.0
6	269.5
7	330.0
8	322.0
9	314.0
10	471.5
11	629.0
12	629.0
13	996.5
14	1364.0
15	2066.0
16	2768.0
17	2624.0
18	2480.0
19	2480.0
20	2699.0
21	2918.0
22	2684.5
23	2451.0
24	2919.0
25	3387.0
26	3387.0
27	4333.5
28	5280.0
29	6929.5
30	8579.0
31	10706.5
32	12834.0
33	12834.0
34	16635.5
35	20437.0
36	25884.5
37	31332.0
38	37983.0
39	44634.0
40	44634.0
41	49510.5
42	54387.0
43	58979.5
44	63572.0
45	72202.5
46	80833.0
47	80833.0
48	88174.5
49	95516.0
50	98958.5
51	102401.0
52	105598.0
53	108795.0
54	108795.0
55	106604.5
56	104414.0
57	101024.5
58	97635.0
59	93848.0
60	90061.0
61	90061.0
62	84856.5
63	79652.0
64	73133.0
65	66614.0
66	59431.5
67	52249.0
68	52249.0
69	43164.0
70	34079.0
71	28545.5
72	23012.0
73	17280.5
74	11549.0
75	11549.0
76	8824.5
77	6100.0
78	4957.5
79	3815.0
80	3430.5
81	3046.0
82	3046.0
83	2636.5
84	2227.0
85	1844.5
86	1462.0
87	1181.5
88	901.0
89	901.0
90	665.5
91	430.0
92	273.5
93	117.0
94	84.5
95	52.0
96	52.0
97	33.5
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1222981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.25039614416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.84226510203763	36.65803048244686
2	7.745930109049551	6.855209516608382
3	2.8537880519517502	3.7884375543100672
4	1.4996932180241749	2.654480759691194
5	0.9490501418311448	2.0997922368349697
6	0.6451315933673484	1.712839714297099
7	0.47901321567237554	1.4837567186253398
8	0.3583426607336008	1.268544375425133
9	0.28073219966397417	1.118025994099705
>10	1.8949878562083295	16.612811414556447
>50	0.26092548295686163	7.968688729400652
>100	0.18141322304676574	15.00094321636113
>500	0.007427357835445814	2.073784686477919
>1k	0.0012997876212030174	0.7046546008651148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1512	0.12363233770598236	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1392	0.11382024741185676	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1286	0.10515290098537916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.453022573531396E-4	0.0
11	0.0	0.0	0.0	4.906045147062792E-4	0.0
12	0.0	0.0	0.0	4.906045147062792E-4	0.0
13	0.0	0.0	0.0	5.723719338239923E-4	0.0
14	0.0	0.0	0.0	8.17674191177132E-4	0.0
15	0.0	0.0	0.0	8.994416102948451E-4	0.0
16	0.0	0.0	0.0	0.0011447438676479846	0.0
17	0.0	0.0	0.0	0.0015535809632365508	0.0
18	0.0	0.0	0.0	0.0015535809632365508	0.0
19	0.0	0.0	0.0	0.001717115801471977	0.0
20	0.0	0.0	0.0	0.0019624180588251167	0.0
21	0.0	0.0	0.0	0.002534789992649109	0.0
22	0.0	0.0	0.0	0.003924836117650233	0.0
23	0.0	0.0	0.0	0.006214323852946203	0.0
24	0.0	0.0	0.0	0.011120369000008994	0.0
25	0.0	0.0	0.0	0.015290507375012367	0.0
26	0.0	0.0	0.0	0.025756737022079656	0.0
27	0.0	0.0	0.0	0.08888118458095425	0.0
28	0.0	0.0	0.0	0.34366846255174854	0.0
29	0.0	0.0	0.0	0.7049169202138055	0.0
30	0.0	0.0	0.0	1.186281716559783	0.0
31	0.0	0.0	0.0	2.2483587234797597	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATAC	40	5.940435E-5	27.750002	3
TTTAGAC	50	2.7022394E-4	22.2	3
GAATCTA	200	0.0	21.275002	13
TCTAGAC	200	0.0	21.275002	3
AATCTAC	200	0.0	21.275002	14
ATTAGAC	45	0.0038259458	20.555557	3
CGACTTG	45	0.0038259458	20.555557	6
GGTATCA	1115	0.0	20.408073	1
TATACAC	945	0.0	19.968254	37
CTAGACA	215	0.0	19.790697	4
ATACACT	85	1.245442E-6	19.588234	4
ATACACA	550	0.0	19.509092	37
GCAGTCG	240	0.0	19.270832	9
TAGAACT	265	0.0	18.849056	4
GTGCTTA	60	9.237278E-4	18.5	1
TATTAGA	70	1.2192788E-4	18.5	2
GGACCTA	60	9.237278E-4	18.5	6
AATAACG	180	0.0	18.5	2
CGCCAGT	60	9.237278E-4	18.5	23
AAGACGG	330	0.0	18.499998	5
>>END_MODULE