FastQCFastQC Report
Fri 10 Feb 2017
ERR1631471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30709
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1780.5796346347976163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.5210198964472955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1580.5145071477417044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1310.42658504021622323No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1100.35820117880751573No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1080.3516884301019245No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1030.3354065583379465No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1010.3288938096323553No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA970.31586831222117295No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA950.30935556351558174No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT940.30609918916278617No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA800.26050994822364776No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT780.2539971995180566No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT690.22468983034289622No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA630.20515158422612262No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT600.19538246116773583No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT530.17258784069816666No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA530.17258784069816666No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT510.16607509199257547RNA PCR Primer, Index 39 (95% over 23bp)
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA480.1563059689341887No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA460.14979322022859748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.14979322022859748No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC460.14979322022859748No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT460.14979322022859748No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT450.14653684587580187No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT430.1400240971702107No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT430.1400240971702107No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA430.1400240971702107No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA400.13025497411182388No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG380.1237422254062327No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT370.1204858510534371No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA360.11722947670064152No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC360.11722947670064152ABI Solid3 Adapter B (100% over 23bp)
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC350.11397310234784591No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT350.11397310234784591No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA350.11397310234784591No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC320.10420397928945911No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT310.10094760493666352No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG310.10094760493666352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG200.001814176237.06
AAATCTC200.001814176237.05
GAAATCT200.001814176237.04
AGAAATC200.001814176237.03
TTGAAAA200.001814176237.028
CAGAAAT200.001814176237.02
CTTGAAA200.001814176237.027
TCTTCTG200.001814176237.020
CTTCTGC200.001814176237.021
TATTGAC401.5339974E-632.37530
ATTGACT401.5339974E-632.37531
TGCCGTC250.00541444929.59999815
TTCTGCT250.00541444929.59999822
CCGTCTT250.00541444929.59999817
TGCTTGA250.00541444929.59999825
GTCTTCT250.00541444929.59999819
CTGCTTG250.00541444929.59999824
CGTCTTC250.00541444929.59999818
TGAAAAA250.00541444929.59999829
TTGACTG555.935035E-726.9090932
Produced by FastQC (version 0.11.5)