##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30709
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70673092578723	34.0	31.0	34.0	31.0	34.0
2	32.883486925656975	34.0	33.0	34.0	31.0	34.0
3	32.8827053958123	34.0	33.0	34.0	31.0	34.0
4	36.327167931225375	37.0	37.0	37.0	35.0	37.0
5	36.32231593343971	37.0	37.0	37.0	35.0	37.0
6	36.37956299456185	37.0	37.0	37.0	35.0	37.0
7	36.387345729265036	37.0	37.0	37.0	35.0	37.0
8	36.38617343449803	37.0	37.0	37.0	35.0	37.0
9	38.200657787619264	39.0	39.0	39.0	37.0	39.0
10	38.18913022241037	39.0	39.0	39.0	37.0	39.0
11	38.24833110814419	39.0	39.0	39.0	37.0	39.0
12	38.029698134097494	39.0	38.0	39.0	37.0	39.0
13	38.141261519424276	39.0	39.0	39.0	37.0	39.0
14	39.56491582272298	41.0	39.0	41.0	37.0	41.0
15	39.548438568497836	41.0	39.0	41.0	37.0	41.0
16	39.45091015663161	40.0	39.0	41.0	37.0	41.0
17	39.3968543423752	40.0	39.0	41.0	37.0	41.0
18	39.50805952652317	41.0	39.0	41.0	37.0	41.0
19	39.54410759060862	41.0	39.0	41.0	37.0	41.0
20	39.564818131492395	41.0	39.0	41.0	37.0	41.0
21	39.581132566999905	41.0	39.0	41.0	37.0	41.0
22	39.51375818164056	41.0	39.0	41.0	37.0	41.0
23	39.483441336416035	41.0	39.0	41.0	37.0	41.0
24	39.441824872187304	41.0	39.0	41.0	37.0	41.0
25	39.38786674916148	41.0	39.0	41.0	36.0	41.0
26	39.29740466964082	41.0	39.0	41.0	36.0	41.0
27	39.21156664170113	41.0	39.0	41.0	35.0	41.0
28	39.16223257025628	41.0	39.0	41.0	35.0	41.0
29	39.04708717314143	40.0	39.0	41.0	35.0	41.0
30	38.94727929922824	40.0	39.0	41.0	35.0	41.0
31	38.84988114233612	40.0	38.0	41.0	35.0	41.0
32	38.70699143573545	40.0	38.0	41.0	35.0	41.0
33	38.600312611937866	40.0	38.0	41.0	35.0	41.0
34	38.57540134813898	40.0	38.0	41.0	35.0	41.0
35	38.443290240646064	40.0	38.0	41.0	35.0	41.0
36	38.402455306262006	40.0	38.0	41.0	35.0	41.0
37	38.280341268032174	40.0	38.0	41.0	35.0	41.0
38	38.16066951056693	40.0	37.0	41.0	34.0	41.0
39	37.99407339867791	40.0	37.0	41.0	34.0	41.0
40	37.91712527272135	40.0	37.0	41.0	34.0	41.0
41	37.75479501123449	40.0	37.0	41.0	33.0	41.0
42	37.61216581458204	40.0	36.0	41.0	33.0	41.0
43	36.69969715718519	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	4.0
22	4.0
23	15.0
24	26.0
25	38.0
26	70.0
27	80.0
28	129.0
29	167.0
30	243.0
31	301.0
32	398.0
33	506.0
34	744.0
35	1055.0
36	1648.0
37	3393.0
38	7060.0
39	14824.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.443583314337815	15.142140740499526	15.70874987788596	26.70552606727669
2	21.713504184441042	17.659318115210525	34.664104985509134	25.963072714839296
3	19.088866456087793	21.202253411052137	30.44710019863884	29.261779934221238
4	14.826272428278356	18.818587384805756	32.70051125077339	33.6546289361425
5	15.555700283304569	35.452147578885665	32.277182584909966	16.714969552899802
6	29.782799830668534	31.166758930606665	19.870396300758735	19.180044937966066
7	30.818326874857533	30.766224885212807	18.170568888599433	20.244879351330226
8	31.808264678107395	29.372496662216292	17.72770197661923	21.091536683057086
9	29.60044286691198	13.338109349050766	16.503305219968087	40.558142564069165
10	21.651633071737926	23.494740955420234	24.660522973721058	30.19310299912078
11	34.09098309941711	24.862418183594386	16.0506691849295	24.995929532059005
12	24.86567455794718	28.42489172555277	23.530561073300987	23.17887264319906
13	34.462209775635806	17.799342212380736	24.028786349278715	23.709661662704747
14	24.214399687388063	20.922205216711713	22.794620469569182	32.06877462633104
15	33.820704028135076	22.351753557588978	18.899996743625646	24.927545670650296
16	26.51014360610896	24.699599465954606	23.836660262463774	24.953596665472663
17	21.50835260021492	28.297893125793745	24.214399687388063	25.979354586603275
18	25.520205802859095	21.172946041876976	25.91748347390016	27.38936468136377
19	25.171773747109967	28.262073007912992	25.018724152528577	21.547429092448468
20	27.32423719430786	20.625875150607314	28.271842130971375	23.778045524113452
21	30.994171089908495	19.50893874759842	24.61493373278192	24.88195642971116
22	29.60695561561757	20.06903513627927	22.394086424175324	27.92992282392784
23	26.56550197010648	22.127063727246085	22.569930639226286	28.737503663421148
24	24.74844508124654	23.130027027907126	26.685987821159923	25.435540069686414
25	24.562831743137192	21.69396593832427	24.351167410205477	29.392034908333066
26	28.57142857142857	20.974307206356443	22.86300433097789	27.591259891237097
27	26.24312090917972	21.452994236217396	22.77182584909961	29.532059005503275
28	24.66703572242665	21.023152821648377	26.94649776938357	27.363313686541407
29	24.875443681005567	21.843759158552867	28.02435768015891	25.256439480282655
30	23.92783874434205	22.615519880165426	29.43111140056661	24.02552997492592
31	24.71913771207138	23.989709857045167	25.188055618873946	26.103096812009507
32	22.576443387931878	23.273307499430135	29.88700380995799	24.263245302679994
33	23.885505877755705	21.303201015988797	30.13448826077046	24.676804845485037
34	26.04122569930639	22.703441987690905	24.536780748314825	26.718551564687875
35	23.716174411410336	23.361229606955618	26.17799342212381	26.744602559510238
36	20.958025334592463	22.595981634048652	27.89735908039988	28.548633950959
37	22.1172946041877	21.710247810088248	29.281318180338012	26.891139405386046
38	22.14334559901006	18.196619883421796	31.44355075059429	28.216483766973848
39	22.31593343970823	18.362694975414374	33.15640366016478	26.164967924712624
40	21.34553388257514	17.30111693640301	31.749649939757074	29.603699241264774
41	17.49975577192354	19.860627177700348	34.61200299586441	28.02761405451171
42	17.356475300400533	19.24517242502198	33.70998730014003	29.68836497443746
43	15.458009052720701	22.273600573121886	35.39027646618255	26.87811390797486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	9.5
4	16.0
5	16.0
6	25.5
7	35.0
8	36.0
9	37.0
10	63.0
11	89.0
12	89.0
13	133.5
14	178.0
15	262.0
16	346.0
17	310.0
18	274.0
19	274.0
20	281.0
21	288.0
22	198.0
23	108.0
24	86.5
25	65.0
26	65.0
27	83.5
28	102.0
29	134.5
30	167.0
31	196.5
32	226.0
33	226.0
34	307.0
35	388.0
36	465.0
37	542.0
38	667.0
39	792.0
40	792.0
41	976.0
42	1160.0
43	1489.5
44	1819.0
45	2303.5
46	2788.0
47	2788.0
48	3569.5
49	4351.0
50	4110.0
51	3869.0
52	3134.0
53	2399.0
54	2399.0
55	2054.0
56	1709.0
57	1621.0
58	1533.0
59	1470.5
60	1408.0
61	1408.0
62	1359.5
63	1311.0
64	1265.0
65	1219.0
66	1145.5
67	1072.0
68	1072.0
69	927.5
70	783.0
71	700.0
72	617.0
73	520.0
74	423.0
75	423.0
76	330.5
77	238.0
78	189.0
79	140.0
80	115.0
81	90.0
82	90.0
83	74.0
84	58.0
85	43.0
86	28.0
87	25.0
88	22.0
89	22.0
90	15.5
91	9.0
92	7.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	30709.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.18050083037546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73879766831308	65.67130157282881
2	6.01610821875139	8.805236249959297
3	1.7176166955902639	3.7708815005373015
4	0.7475637431584568	2.1882835650786414
5	0.4004805766920304	1.4653684587580187
6	0.2758866194989543	1.2113712592399624
7	0.2002402883460152	1.0257579211306131
8	0.12014417300760913	0.703376860203849
9	0.0934454678948071	0.6154547526783679
>10	0.6051706492235126	8.544726301735647
>50	0.048947626040137054	2.5823048617669087
>100	0.03559827348373604	3.4159366960825817
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	178	0.5796346347976163	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	160	0.5210198964472955	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.5145071477417044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.42658504021622323	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	110	0.35820117880751573	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	108	0.3516884301019245	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	103	0.3354065583379465	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	101	0.3288938096323553	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	97	0.31586831222117295	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	95	0.30935556351558174	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	94	0.30609918916278617	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	80	0.26050994822364776	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	78	0.2539971995180566	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	69	0.22468983034289622	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	63	0.20515158422612262	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	60	0.19538246116773583	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	53	0.17258784069816666	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	53	0.17258784069816666	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	51	0.16607509199257547	RNA PCR Primer, Index 39 (95% over 23bp)
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	48	0.1563059689341887	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	46	0.14979322022859748	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.14979322022859748	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	46	0.14979322022859748	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	46	0.14979322022859748	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	45	0.14653684587580187	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	43	0.1400240971702107	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	43	0.1400240971702107	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	43	0.1400240971702107	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	40	0.13025497411182388	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	38	0.1237422254062327	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	37	0.1204858510534371	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	36	0.11722947670064152	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	36	0.11722947670064152	ABI Solid3 Adapter B (100% over 23bp)
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	35	0.11397310234784591	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	35	0.11397310234784591	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	35	0.11397310234784591	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC	32	0.10420397928945911	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	31	0.10094760493666352	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	31	0.10094760493666352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0032563743527955972	0.0
16	0.0	0.0	0.0	0.0032563743527955972	0.0
17	0.0	0.0	0.0	0.0065127487055911945	0.0
18	0.0	0.0	0.0	0.0065127487055911945	0.0
19	0.0	0.0	0.0	0.009769123058386793	0.0
20	0.0	0.0	0.0	0.009769123058386793	0.0
21	0.0	0.0	0.0	0.009769123058386793	0.0
22	0.0	0.0	0.0	0.009769123058386793	0.0
23	0.0	0.0	0.0	0.03907649223354717	0.0
24	0.0	0.0	0.0	0.07815298446709434	0.0
25	0.0	0.0	0.0	0.09443485623107233	0.0
26	0.0	0.0	0.0	0.1237422254062327	0.0
27	0.0	0.0	0.0	0.2442280764596698	0.0
28	0.0	0.0	0.0	0.5047380246833176	0.0
29	0.0	0.0	0.0	1.0257579211306131	0.0
30	0.0	0.0	0.0	1.7747240222736005	0.0
31	0.0	0.0	0.0	3.2303233579732327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTCG	20	0.0018141762	37.0	6
AAATCTC	20	0.0018141762	37.0	5
GAAATCT	20	0.0018141762	37.0	4
AGAAATC	20	0.0018141762	37.0	3
TTGAAAA	20	0.0018141762	37.0	28
CAGAAAT	20	0.0018141762	37.0	2
CTTGAAA	20	0.0018141762	37.0	27
TCTTCTG	20	0.0018141762	37.0	20
CTTCTGC	20	0.0018141762	37.0	21
TATTGAC	40	1.5339974E-6	32.375	30
ATTGACT	40	1.5339974E-6	32.375	31
TGCCGTC	25	0.005414449	29.599998	15
TTCTGCT	25	0.005414449	29.599998	22
CCGTCTT	25	0.005414449	29.599998	17
TGCTTGA	25	0.005414449	29.599998	25
GTCTTCT	25	0.005414449	29.599998	19
CTGCTTG	25	0.005414449	29.599998	24
CGTCTTC	25	0.005414449	29.599998	18
TGAAAAA	25	0.005414449	29.599998	29
TTGACTG	55	5.935035E-7	26.90909	32
>>END_MODULE