FastQCFastQC Report
Fri 10 Feb 2017
ERR1631470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220842
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.5121308446762844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8360.37855118138759836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7520.34051493828166746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.1231649776763478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAGA200.00183871837.01
TGCTCGA200.00183871837.010
ATACTTT250.005486848829.66
TCTAGCG358.8466256E-426.4285728
CCGAAGT400.001926276723.1254
GGTTAAT400.001926276723.1251
AGTCGGT1450.021.68965511
GGTATCA5100.020.676471
TCGTTTA450.00381617220.55555730
CCTGTAG450.00381617220.5555573
CGGTCCA450.00381617220.55555710
CTGTAGT555.125028E-420.1818184
GCAGTCG1500.019.7333349
CAGTCGG1600.019.6562510
TATACAC1355.4569682E-1119.18518637
GGCAGTC1551.8189894E-1219.0967758
GCCTTAT1306.85759E-1018.526
TGAACTG500.00701733218.55
GGTCCAA609.204636E-418.511
CGCCTTA1306.85759E-1018.525
Produced by FastQC (version 0.11.5)