##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220842
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19897936080999	33.0	31.0	34.0	31.0	34.0
2	32.39751496545041	34.0	31.0	34.0	31.0	34.0
3	32.51040562936398	34.0	31.0	34.0	31.0	34.0
4	36.102937846967514	37.0	35.0	37.0	35.0	37.0
5	35.86290198422402	37.0	35.0	37.0	35.0	37.0
6	35.88048469041215	37.0	35.0	37.0	35.0	37.0
7	35.887027829851206	37.0	35.0	37.0	35.0	37.0
8	35.86307858106701	37.0	35.0	37.0	35.0	37.0
9	37.61323932947537	39.0	37.0	39.0	35.0	39.0
10	37.47206147381386	39.0	37.0	39.0	35.0	39.0
11	37.562565091785075	39.0	37.0	39.0	35.0	39.0
12	37.315651008413255	39.0	37.0	39.0	35.0	39.0
13	37.47127810833084	39.0	37.0	39.0	35.0	39.0
14	38.726342815225365	40.0	38.0	41.0	35.0	41.0
15	38.69173436212315	40.0	38.0	41.0	35.0	41.0
16	38.64276722724844	40.0	38.0	41.0	35.0	41.0
17	38.6311073074868	40.0	38.0	41.0	35.0	41.0
18	38.627702158103986	40.0	38.0	41.0	35.0	41.0
19	38.63952509033608	40.0	38.0	41.0	35.0	41.0
20	38.62148051548166	40.0	38.0	41.0	35.0	41.0
21	38.58722525606542	40.0	38.0	41.0	35.0	41.0
22	38.532747394064536	40.0	38.0	41.0	34.0	41.0
23	38.45012271216526	40.0	38.0	41.0	34.0	41.0
24	38.39480261906702	40.0	38.0	41.0	34.0	41.0
25	38.38475471151321	40.0	38.0	41.0	34.0	41.0
26	38.253181007235945	40.0	38.0	41.0	34.0	41.0
27	38.17186948134866	40.0	38.0	41.0	34.0	41.0
28	38.075914001865584	40.0	38.0	41.0	34.0	41.0
29	37.96075021961402	40.0	37.0	41.0	33.0	41.0
30	37.866596933554305	40.0	37.0	41.0	33.0	41.0
31	37.780748227239386	40.0	37.0	41.0	33.0	41.0
32	37.68080799847855	40.0	37.0	41.0	33.0	41.0
33	37.545915179177875	40.0	37.0	41.0	33.0	41.0
34	37.483286693654286	40.0	36.0	41.0	33.0	41.0
35	37.36811838327854	39.0	36.0	41.0	32.0	41.0
36	37.29212287517773	39.0	36.0	41.0	32.0	41.0
37	37.21268599270066	39.0	36.0	41.0	32.0	41.0
38	37.100732650492205	39.0	36.0	41.0	32.0	41.0
39	37.00020829371225	39.0	35.0	40.0	31.0	41.0
40	36.874435116508636	39.0	35.0	40.0	31.0	41.0
41	36.736010360348125	39.0	35.0	40.0	31.0	41.0
42	36.57906557629436	39.0	35.0	40.0	31.0	41.0
43	35.54514992619158	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0
17	7.0
18	14.0
19	19.0
20	58.0
21	79.0
22	142.0
23	226.0
24	329.0
25	473.0
26	737.0
27	1054.0
28	1387.0
29	1873.0
30	2600.0
31	3426.0
32	4635.0
33	6184.0
34	8905.0
35	13251.0
36	21782.0
37	39335.0
38	64452.0
39	49868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99978265003939	17.465427771891218	12.48132148776048	26.053468090308908
2	19.61085300803289	20.421387236123564	32.82120248865705	27.146557267186495
3	20.204490087936172	21.853180101611105	29.470390595991702	28.471939214461017
4	14.863567618478369	15.746099021019552	33.13454868186305	36.255784678639024
5	15.904130554876335	35.582452613180465	32.19903822642432	16.31437860551888
6	34.603472165620666	33.785692938843155	16.086613959301218	15.524220936234956
7	30.603327265646936	30.1736082810335	19.600891134838484	19.62217331848109
8	29.851205839468943	31.0760634299635	19.034422800010866	20.038307930556687
9	28.173083018628702	13.724291574972153	18.427201347569756	39.67542405882939
10	18.910352197498664	25.596580360619807	29.769699604241946	25.72336783763958
11	37.690747231052065	21.13456679435977	19.582325825703446	21.592360148884723
12	23.643600402097427	25.166861376006377	26.800155767471768	24.389382454424428
13	31.866673911665355	17.9562764329249	24.11950625333949	26.05754340207026
14	23.98728502730459	19.69598174260331	23.697937892248756	32.618795337843345
15	27.316814736327327	25.648653788681504	21.113284610717166	25.921246864274007
16	26.352777098559148	24.662428342434865	22.892384600755292	26.092409958250695
17	25.074034830331186	25.40322945816466	23.675750083770296	25.846985627733854
18	25.377871962760707	23.1536573658996	25.625560355367185	25.842910315972507
19	25.88592749567564	24.253991541464035	25.19584137075375	24.664239592106576
20	26.572843933672036	22.91683647132339	24.954039539580332	25.556280055424242
21	27.685404044520517	23.443004500955432	23.90442035482381	24.967171099700238
22	27.43499877740647	23.164977676347796	23.682995082457143	25.717028463788587
23	25.90268155513897	23.543528857735392	24.432399634127567	26.12138995299807
24	25.5082819391239	23.897628168554892	24.705445522138	25.888644370183204
25	25.742385959192543	23.04905769735829	24.50983055759321	26.698725785855952
26	25.671747221995815	23.734615698100907	24.6646924045245	25.92894467537878
27	26.21240524900155	23.155468615571316	24.32463027866076	26.307495856766376
28	25.14150388060242	23.622318218454826	25.02105577743364	26.215122123509115
29	24.847628621367313	23.932041912317402	25.477943507122742	25.742385959192543
30	24.431946821709637	24.65473053133009	25.50601787703426	25.407304769926007
31	25.44171851368852	24.251274666956466	24.531565553653746	25.77544126570127
32	24.455945879859808	24.22999248331386	25.2619519837712	26.052109653055126
33	24.4835674373534	23.45794731074705	25.82389219441954	26.23459305748001
34	25.381041649686203	23.60420572173771	25.3728910261635	25.641861602412586
35	25.050488584598945	23.451155124478134	25.976489979261192	25.52186631166173
36	23.932041912317402	23.79348131243151	25.954302170782732	26.320174604468356
37	24.43738057072477	22.525606542233813	26.467791452712795	26.569221434328615
38	23.853252551597976	22.075510998813634	27.583974062904705	26.487262386683692
39	23.895364106465255	21.77031542913033	28.06667210041568	26.267648363988734
40	23.414024506208058	21.693337318082612	28.490504523596055	26.402133652113275
41	21.994910388422493	21.998532887765915	29.69860805462729	26.307948669184306
42	22.342670325391005	21.459686110431893	29.99927550013132	26.19836806404579
43	21.11826554731437	21.037664936923232	30.355186060622525	27.488883455139874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	28.5
2	37.0
3	66.0
4	95.0
5	95.0
6	150.0
7	205.0
8	201.5
9	198.0
10	311.5
11	425.0
12	425.0
13	645.0
14	865.0
15	1511.5
16	2158.0
17	1940.0
18	1722.0
19	1722.0
20	1913.5
21	2105.0
22	1720.0
23	1335.0
24	1485.0
25	1635.0
26	1635.0
27	1859.0
28	2083.0
29	2523.5
30	2964.0
31	3412.5
32	3861.0
33	3861.0
34	4601.5
35	5342.0
36	6169.5
37	6997.0
38	7951.0
39	8905.0
40	8905.0
41	9841.5
42	10778.0
43	11403.0
44	12028.0
45	12923.0
46	13818.0
47	13818.0
48	15014.5
49	16211.0
50	16587.5
51	16964.0
52	17383.0
53	17802.0
54	17802.0
55	16829.0
56	15856.0
57	15245.0
58	14634.0
59	14033.5
60	13433.0
61	13433.0
62	12743.5
63	12054.0
64	11149.0
65	10244.0
66	9164.5
67	8085.0
68	8085.0
69	6950.5
70	5816.0
71	5066.5
72	4317.0
73	3522.0
74	2727.0
75	2727.0
76	2248.0
77	1769.0
78	1498.5
79	1228.0
80	1041.0
81	854.0
82	854.0
83	716.5
84	579.0
85	448.5
86	318.0
87	270.5
88	223.0
89	223.0
90	163.5
91	104.0
92	67.5
93	31.0
94	22.0
95	13.0
96	13.0
97	7.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	220842.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.50838597882911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.7609600437809	67.45200068686162
2	4.396594745225082	6.463731670489943
3	1.21672725152155	2.68318969307418
4	0.6536583622343067	1.9219748475763485
5	0.3727474552762297	1.370003190753572
6	0.28826779028682675	1.2714059996200937
7	0.20632522751815358	1.061664412310192
8	0.13678088374668237	0.8043633597581183
9	0.12682181612001517	0.8390220308897552
>10	0.7650465059711329	10.447216293525202
>50	0.06370082590949126	3.30096684517342
>100	0.010513728548168462	1.148360040064462
>500	0.0012369092409609953	0.7219302231284108
>1k	6.184546204804977E-4	0.5141707067747057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1131	0.5121308446762844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	836	0.37855118138759836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.34051493828166746	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	272	0.1231649776763478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.5281241792774924E-4	0.0	0.0	0.0
4	0.0	4.5281241792774924E-4	0.0	0.0	0.0
5	0.0	4.5281241792774924E-4	0.0	0.0	0.0
6	0.0	4.5281241792774924E-4	0.0	0.0	0.0
7	0.0	4.5281241792774924E-4	0.0	0.0	0.0
8	0.0	4.5281241792774924E-4	0.0	4.5281241792774924E-4	0.0
9	0.0	4.5281241792774924E-4	0.0	4.5281241792774924E-4	0.0
10	0.0	4.5281241792774924E-4	0.0	9.056248358554985E-4	0.0
11	0.0	4.5281241792774924E-4	0.0	9.056248358554985E-4	0.0
12	0.0	4.5281241792774924E-4	0.0	9.056248358554985E-4	0.0
13	0.0	4.5281241792774924E-4	0.0	0.0013584372537832478	0.0
14	0.0	4.5281241792774924E-4	0.0	0.0013584372537832478	0.0
15	0.0	4.5281241792774924E-4	0.0	0.0022640620896387464	0.0
16	0.0	4.5281241792774924E-4	0.0	0.0027168745075664955	4.5281241792774924E-4
17	0.0	4.5281241792774924E-4	0.0	0.0031696869254942447	4.5281241792774924E-4
18	0.0	4.5281241792774924E-4	0.0	0.0031696869254942447	4.5281241792774924E-4
19	0.0	4.5281241792774924E-4	0.0	0.0031696869254942447	4.5281241792774924E-4
20	0.0	4.5281241792774924E-4	0.0	0.0040753117613497435	4.5281241792774924E-4
21	0.0	4.5281241792774924E-4	0.0	0.0040753117613497435	4.5281241792774924E-4
22	0.0	4.5281241792774924E-4	0.0	0.0063393738509884895	4.5281241792774924E-4
23	0.0	4.5281241792774924E-4	0.0	0.007697811104771737	4.5281241792774924E-4
24	0.0	4.5281241792774924E-4	0.0	0.01222593528404923	4.5281241792774924E-4
25	0.0	4.5281241792774924E-4	0.0	0.016301247045398974	4.5281241792774924E-4
26	0.0	4.5281241792774924E-4	0.0	0.02399905815017071	4.5281241792774924E-4
27	0.0	4.5281241792774924E-4	0.0	0.06746905027123463	9.056248358554985E-4
28	0.0	4.5281241792774924E-4	0.0	0.22595339654594687	9.056248358554985E-4
29	0.0	4.5281241792774924E-4	0.0	0.4650383532117985	9.056248358554985E-4
30	0.0	4.5281241792774924E-4	0.0	0.7634417366261852	0.0013584372537832478
31	0.0	4.5281241792774924E-4	0.0	1.4503581746225809	0.0013584372537832478
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTAGA	20	0.001838718	37.0	1
TGCTCGA	20	0.001838718	37.0	10
ATACTTT	25	0.0054868488	29.6	6
TCTAGCG	35	8.8466256E-4	26.42857	28
CCGAAGT	40	0.0019262767	23.125	4
GGTTAAT	40	0.0019262767	23.125	1
AGTCGGT	145	0.0	21.689655	11
GGTATCA	510	0.0	20.67647	1
TCGTTTA	45	0.003816172	20.555557	30
CCTGTAG	45	0.003816172	20.555557	3
CGGTCCA	45	0.003816172	20.555557	10
CTGTAGT	55	5.125028E-4	20.181818	4
GCAGTCG	150	0.0	19.733334	9
CAGTCGG	160	0.0	19.65625	10
TATACAC	135	5.4569682E-11	19.185186	37
GGCAGTC	155	1.8189894E-12	19.096775	8
GCCTTAT	130	6.85759E-10	18.5	26
TGAACTG	50	0.007017332	18.5	5
GGTCCAA	60	9.204636E-4	18.5	11
CGCCTTA	130	6.85759E-10	18.5	25
>>END_MODULE