FastQCFastQC Report
Fri 10 Feb 2017
ERR1631469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134406
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26781.992470574230317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18231.3563382587086887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17641.3124414088656757No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT7850.5840513072333081No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6960.5178340252667292No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT6500.48360936267726146No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT6350.4724491466154785No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT6210.4620329449578144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.4337603976012976No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC5470.40697587905301846No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC5400.4017677782241864No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA4310.3206702081752303No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA4010.29834977605166435No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.29834977605166435No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3730.2775173727363362No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA3400.25296489740041367No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3390.2522208829962948No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT3300.24552475335922505No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT3260.2425486957427496No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3030.22543636444801574No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2970.22097227802330252No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA2940.21874023481094595No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2810.2090680475574007No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT2690.20013987470797434No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG2570.19121170185854797No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT2400.1785634569885273No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT2330.17335535615969525No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT2300.17112331294733865No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC2290.17037929854321981No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2230.16591521211850663No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC2210.1644271833102689No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2200.16368316890615No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.1599630968855557No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.15847506807731798No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.15847506807731798No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT2100.1562430248649614No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT2070.1540109816526048No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1960.1458268232072973No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1960.1458268232072973No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1920.14285076559082185No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT1910.14210675118670296No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT1850.13764266476198977No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT1730.12871449191256343No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.12797047750844456No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1660.12350639108373138No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1580.11755427585078046No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA1580.11755427585078046No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA1480.11011413180959183No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1470.10937011740547298No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1460.1086261030013541No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.10713807419311638No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT1420.10565004538487865No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT1410.10490603098075979No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT1390.10341800217252206No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC1390.10341800217252206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACT1400.030.39285731
TATTGAC1350.030.1481530
TCACTGT801.8189894E-1230.062535
GCTCTAT250.005479303729.61
ATCACTG702.0372681E-1029.07142834
TATCACT754.5292836E-1027.13333333
AGTGCTG358.8285375E-426.4285727
ATGCCCC1200.026.20833437
ATACACT659.2633854E-825.6153854
GATACAC601.3227836E-624.6666683
TTGACTG1650.024.66666632
TACACTG701.8967694E-723.7857135
TGACTGT1750.023.25714333
ACCGACC802.6806447E-823.12532
GTATCAC753.6901656E-722.232
GAGGGCA1005.2204996E-1022.29
AGGGCAA1059.604264E-1021.14285710
TGGGCAG8200.020.9817076
GGGCAGT8250.020.8545467
TGATACA806.866503E-720.81252
Produced by FastQC (version 0.11.5)