##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134406
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34746216686755	31.0	31.0	34.0	30.0	34.0
2	31.512291117956043	31.0	31.0	34.0	30.0	34.0
3	31.41236998348288	31.0	31.0	34.0	28.0	34.0
4	35.415532044700385	37.0	35.0	37.0	33.0	37.0
5	34.71273603856971	35.0	35.0	37.0	31.0	37.0
6	34.67883874231805	35.0	35.0	37.0	31.0	37.0
7	34.7194916893591	35.0	35.0	37.0	32.0	37.0
8	34.676495096945075	35.0	35.0	37.0	32.0	37.0
9	36.19993155067482	37.0	35.0	39.0	32.0	39.0
10	35.99454637441781	37.0	35.0	39.0	31.0	39.0
11	36.12413136318319	37.0	35.0	39.0	32.0	39.0
12	35.81842328467479	37.0	35.0	39.0	30.0	39.0
13	35.95257652188146	37.0	35.0	39.0	31.0	39.0
14	36.74495186226805	38.0	36.0	40.0	31.0	41.0
15	36.608142493638674	38.0	36.0	40.0	31.0	41.0
16	36.44416171897088	38.0	35.0	40.0	31.0	41.0
17	36.497165305120305	38.0	36.0	40.0	31.0	41.0
18	36.720503548948706	38.0	36.0	40.0	31.0	41.0
19	36.739215511212294	38.0	36.0	40.0	31.0	41.0
20	36.75952710444474	38.0	36.0	40.0	31.0	41.0
21	36.77266639882148	38.0	36.0	40.0	31.0	41.0
22	36.677358153653856	38.0	35.0	40.0	31.0	41.0
23	36.59047215154085	38.0	35.0	40.0	31.0	41.0
24	36.512544082853445	38.0	35.0	40.0	30.0	41.0
25	36.50482121333869	38.0	35.0	40.0	30.0	41.0
26	36.291534604109934	38.0	35.0	40.0	30.0	41.0
27	36.16828861806765	38.0	35.0	40.0	30.0	41.0
28	35.94454860646102	38.0	35.0	40.0	29.0	41.0
29	35.69479041114236	38.0	34.0	40.0	29.0	41.0
30	35.42974271981905	38.0	34.0	40.0	28.0	41.0
31	35.20292992872342	37.0	34.0	40.0	28.0	41.0
32	34.97810365608678	37.0	33.0	39.0	27.0	40.0
33	34.76824695326101	37.0	33.0	39.0	27.0	40.0
34	34.68255881433865	37.0	33.0	39.0	26.0	40.0
35	34.49251521509456	37.0	33.0	39.0	26.0	40.0
36	34.37928366293171	37.0	33.0	39.0	25.0	40.0
37	34.18558695296341	36.0	33.0	39.0	24.0	40.0
38	33.988326413999374	36.0	33.0	39.0	24.0	40.0
39	33.66285731291758	36.0	33.0	39.0	23.0	40.0
40	33.46771721500528	36.0	32.0	39.0	22.0	40.0
41	33.13012067913635	35.0	32.0	39.0	21.0	40.0
42	32.94151302769222	35.0	31.0	39.0	19.0	40.0
43	31.8416365340833	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	6.0
17	13.0
18	29.0
19	48.0
20	99.0
21	156.0
22	261.0
23	391.0
24	571.0
25	895.0
26	1345.0
27	1899.0
28	2496.0
29	3331.0
30	4494.0
31	5737.0
32	7434.0
33	9802.0
34	12846.0
35	16598.0
36	20708.0
37	21504.0
38	16805.0
39	6933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87820484204575	13.10209365653319	18.057973602368943	23.961727899052125
2	21.080904126303885	20.384506644048628	33.849679329791826	24.68490989985566
3	18.77892355996012	22.778000982099016	31.526122345728613	26.916953112212255
4	13.197327500260405	20.42989152269988	31.041768968647233	35.331012008392484
5	13.479308959421454	38.99453893427377	30.241209469815335	17.284942636489443
6	26.536017737303396	32.417451601863014	21.462583515616863	19.583947145216733
7	29.709983185274467	32.72324152195586	16.656250465009002	20.910524827760664
8	35.31315566269363	28.92802404654554	16.080383316221003	19.678436974539824
9	31.885481302918024	12.43396872163445	15.136229037394164	40.54432093805336
10	21.709596297784326	26.326949689745994	21.141169293037514	30.82228471943217
11	34.30724818832493	25.919973810692976	14.46512804487895	25.307649956103152
12	25.414044015892145	31.1273306251209	22.850170379298543	20.608454979688407
13	37.22750472449147	17.514099072958054	24.37911997976281	20.879276222787674
14	24.497418270017707	20.909036798952428	24.498906298825947	30.094638632203917
15	33.321429102867434	23.222921595762095	19.70968557951282	23.745963721857656
16	23.429757600107138	25.629064178682498	25.231760486883026	25.70941773432734
17	17.831793223516808	31.095338005743788	25.895421335357057	25.177447435382348
18	23.608321057095665	21.69843608172254	28.609585881582667	26.083656979599123
19	22.99599720250584	29.580524678957786	29.198845289644808	18.22463282889157
20	27.16247786557148	20.82868324330759	31.27166941951996	20.737169471600968
21	31.684597413805932	19.07280924958707	27.64608722824874	21.596506108358255
22	28.962992723539127	20.75874588932042	23.870214127345506	26.408047259794948
23	24.03017722423106	23.89402258827731	24.657381366903262	27.418418820588364
24	21.6612353615166	23.80176480216657	31.354999032781272	23.18200080353556
25	21.214082704641164	22.945404223025758	27.367825841108285	28.472687231224796
26	26.765174173772007	20.941773432733658	26.73392556879901	25.559126824695326
27	24.328527000282726	21.068255881433863	25.309881999315508	29.293335118967907
28	21.968513310417688	21.506480365459876	30.194336562355844	26.33066976176659
29	22.64259036054938	22.278023302531135	31.9948514203235	23.084534916595985
30	21.66644346234543	23.52052735740964	33.78718211984584	21.025847060399087
31	22.2371025103046	25.90881359463119	28.683987321994554	23.170096573069653
32	20.614407094921358	24.763031412288143	33.924824784607836	20.69773670818267
33	23.190184961980865	22.228174337455172	33.37723018317635	21.20441051738762
34	23.824085234290134	24.046545541121674	27.093284525988427	25.036084698599765
35	21.24830736723063	24.96837938782495	28.681011264378075	25.102301980566345
36	18.084014106513102	23.668586223829294	30.7091945300061	27.538205139651506
37	19.29750159963097	23.790604586104788	32.418939630671254	24.492954183592992
38	20.100293141675223	19.027424370935822	33.84744728657947	27.024835200809488
39	19.855512402720116	18.93739862803744	37.008764489680516	24.198324479561926
40	19.719357766766365	18.72014642203473	33.0163831971787	28.544112614020207
41	15.027602934392808	20.59952680683898	38.28400517834025	26.088865080427958
42	15.392169992411054	19.484993229468923	35.71864351293841	29.404193265181615
43	13.338690237042988	22.75493653557133	38.77803074267518	25.128342484710505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	7.0
2	13.0
3	55.5
4	98.0
5	98.0
6	174.5
7	251.0
8	343.0
9	435.0
10	646.0
11	857.0
12	857.0
13	1431.0
14	2005.0
15	3498.0
16	4991.0
17	4363.0
18	3735.0
19	3735.0
20	3954.5
21	4174.0
22	2934.5
23	1695.0
24	1307.0
25	919.0
26	919.0
27	854.5
28	790.0
29	808.0
30	826.0
31	888.0
32	950.0
33	950.0
34	920.5
35	891.0
36	928.5
37	966.0
38	1295.0
39	1624.0
40	1624.0
41	2354.5
42	3085.0
43	4674.0
44	6263.0
45	8603.5
46	10944.0
47	10944.0
48	15125.5
49	19307.0
50	17565.5
51	15824.0
52	12959.0
53	10094.0
54	10094.0
55	8137.0
56	6180.0
57	5982.5
58	5785.0
59	5720.0
60	5655.0
61	5655.0
62	5623.0
63	5591.0
64	5457.0
65	5323.0
66	4930.5
67	4538.0
68	4538.0
69	4169.0
70	3800.0
71	3269.0
72	2738.0
73	2303.5
74	1869.0
75	1869.0
76	1497.5
77	1126.0
78	853.0
79	580.0
80	438.5
81	297.0
82	297.0
83	209.5
84	122.0
85	86.5
86	51.0
87	31.0
88	11.0
89	11.0
90	5.5
91	0.0
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	134406.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.49808788298141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99776163263265	54.14192818772972
2	4.625542397679101	5.504218561671355
3	1.3842864109842563	2.4708718360787465
4	0.7165276544661056	1.7052810142404358
5	0.42141329765284047	1.2536642709402854
6	0.2851104803111206	1.0178117048346056
7	0.2225862521727169	0.9270419475321041
8	0.18882316897797896	0.8987694001755874
9	0.12004651802573497	0.6428284451586983
>10	0.8528304718078256	9.84703063851316
>50	0.09003488851930122	3.7483445679508356
>100	0.08128149657992473	9.418478341740697
>500	0.010003876502144581	3.762480841629094
>1k	0.003751453688304218	4.661250241804681
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2678	1.992470574230317	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1823	1.3563382587086887	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1764	1.3124414088656757	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	785	0.5840513072333081	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	696	0.5178340252667292	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	650	0.48360936267726146	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	635	0.4724491466154785	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	621	0.4620329449578144	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.4337603976012976	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	547	0.40697587905301846	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	540	0.4017677782241864	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	431	0.3206702081752303	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	401	0.29834977605166435	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	401	0.29834977605166435	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	373	0.2775173727363362	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	340	0.25296489740041367	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	339	0.2522208829962948	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	330	0.24552475335922505	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	326	0.2425486957427496	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	303	0.22543636444801574	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	297	0.22097227802330252	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	294	0.21874023481094595	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	281	0.2090680475574007	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	269	0.20013987470797434	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	257	0.19121170185854797	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	240	0.1785634569885273	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	233	0.17335535615969525	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	230	0.17112331294733865	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	229	0.17037929854321981	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	223	0.16591521211850663	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	221	0.1644271833102689	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	220	0.16368316890615	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	215	0.1599630968855557	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	0.15847506807731798	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	213	0.15847506807731798	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	210	0.1562430248649614	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	207	0.1540109816526048	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	196	0.1458268232072973	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	196	0.1458268232072973	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	192	0.14285076559082185	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	191	0.14210675118670296	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	185	0.13764266476198977	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	173	0.12871449191256343	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	172	0.12797047750844456	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	166	0.12350639108373138	No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	158	0.11755427585078046	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	158	0.11755427585078046	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	148	0.11011413180959183	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	147	0.10937011740547298	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	146	0.1086261030013541	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144	0.10713807419311638	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	142	0.10565004538487865	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	141	0.10490603098075979	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	139	0.10341800217252206	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	139	0.10341800217252206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.440144041188638E-4	0.0
2	0.0	0.0	0.0	7.440144041188638E-4	0.0
3	0.0	0.0	0.0	7.440144041188638E-4	0.0
4	0.0	0.0	0.0	7.440144041188638E-4	0.0
5	0.0	0.0	0.0	7.440144041188638E-4	0.0
6	0.0	0.0	7.440144041188638E-4	7.440144041188638E-4	0.0
7	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	0.0
8	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	0.0
9	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	0.0
10	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	0.0
11	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	0.0
12	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	7.440144041188638E-4
13	0.0	0.0	7.440144041188638E-4	0.0014880288082377276	7.440144041188638E-4
14	0.0	0.0	7.440144041188638E-4	0.002232043212356591	7.440144041188638E-4
15	0.0	0.0	7.440144041188638E-4	0.002976057616475455	7.440144041188638E-4
16	0.0	0.0	7.440144041188638E-4	0.005208100828832046	7.440144041188638E-4
17	0.0	0.0	7.440144041188638E-4	0.008184158445307501	0.0014880288082377276
18	0.0	0.0	7.440144041188638E-4	0.010416201657664092	0.0014880288082377276
19	0.0	0.0	7.440144041188638E-4	0.013392259274139548	0.0014880288082377276
20	0.0	0.0	7.440144041188638E-4	0.017112331294733867	0.0014880288082377276
21	0.0	0.0	7.440144041188638E-4	0.020832403315328183	0.0014880288082377276
22	0.0	0.0	7.440144041188638E-4	0.026784518548279096	0.0014880288082377276
23	0.0	0.0	7.440144041188638E-4	0.04240882103477523	0.0014880288082377276
24	0.0	0.0	7.440144041188638E-4	0.07886552683659956	0.0014880288082377276
25	0.0	0.0	7.440144041188638E-4	0.09374581491897684	0.0014880288082377276
26	0.0	0.0	7.440144041188638E-4	0.11755427585078047	0.0014880288082377276
27	0.0	0.0	7.440144041188638E-4	0.2157641771944705	0.0014880288082377276
28	0.0	0.0	7.440144041188638E-4	0.5319702989449876	0.002232043212356591
29	0.0	0.0	7.440144041188638E-4	1.2030712914602026	0.002976057616475455
30	0.0	0.0	7.440144041188638E-4	2.046783625730994	0.002976057616475455
31	0.0	0.0	7.440144041188638E-4	3.6999836316831094	0.002976057616475455
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGACT	140	0.0	30.392857	31
TATTGAC	135	0.0	30.14815	30
TCACTGT	80	1.8189894E-12	30.0625	35
GCTCTAT	25	0.0054793037	29.6	1
ATCACTG	70	2.0372681E-10	29.071428	34
TATCACT	75	4.5292836E-10	27.133333	33
AGTGCTG	35	8.8285375E-4	26.42857	27
ATGCCCC	120	0.0	26.208334	37
ATACACT	65	9.2633854E-8	25.615385	4
GATACAC	60	1.3227836E-6	24.666668	3
TTGACTG	165	0.0	24.666666	32
TACACTG	70	1.8967694E-7	23.785713	5
TGACTGT	175	0.0	23.257143	33
ACCGACC	80	2.6806447E-8	23.125	32
GTATCAC	75	3.6901656E-7	22.2	32
GAGGGCA	100	5.2204996E-10	22.2	9
AGGGCAA	105	9.604264E-10	21.142857	10
TGGGCAG	820	0.0	20.981707	6
GGGCAGT	825	0.0	20.854546	7
TGATACA	80	6.866503E-7	20.8125	2
>>END_MODULE