FastQCFastQC Report
Fri 10 Feb 2017
ERR1631468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465619
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.8788301164686149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36230.7781039863064008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29550.6346390503823942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.2643792456922935No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7510.1612906689804325No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5580.1198404704275384No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.10888730915190317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA200.001840811937.034
GTTGAAT303.59622E-430.83333210
GTTTCCA250.005493027629.637
GAGTCAT250.005493027629.69
GAGTTGG250.005493027629.67
GTGACTC250.005493027629.67
TATGCCG358.861441E-426.4285713
TCCAGGA358.861441E-426.428572
GGTATTG706.5701897E-926.4285733
TGTACGG358.861441E-426.4285710
CCTGTTT509.0759295E-625.90000212
TAAGATA509.0759295E-625.9000024
TCTAGCG451.3215611E-424.66666628
CCAAGCA451.3215611E-424.66666636
CAGTATT451.3215611E-424.66666614
CTAGATA400.001929471623.12513
CGTATGC400.001929471623.12511
CTCGTAT400.001929471623.1259
TCTTCTG400.001929471623.12520
TACCTTA400.001929471623.12531
Produced by FastQC (version 0.11.5)