##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631468.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465619
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55060467893278	34.0	31.0	34.0	31.0	34.0
2	32.73832682944639	34.0	31.0	34.0	31.0	34.0
3	32.82562996784925	34.0	31.0	34.0	31.0	34.0
4	36.28142322370866	37.0	37.0	37.0	35.0	37.0
5	36.22476531241208	37.0	35.0	37.0	35.0	37.0
6	36.291682684770166	37.0	37.0	37.0	35.0	37.0
7	36.27476327211733	37.0	37.0	37.0	35.0	37.0
8	36.27389990528737	37.0	37.0	37.0	35.0	37.0
9	38.05940694000889	39.0	38.0	39.0	37.0	39.0
10	37.97447913422777	39.0	38.0	39.0	35.0	39.0
11	38.06325128484877	39.0	38.0	39.0	37.0	39.0
12	37.84044250771554	39.0	38.0	39.0	35.0	39.0
13	37.9824964187458	39.0	38.0	39.0	35.0	39.0
14	39.38028516877533	40.0	39.0	41.0	37.0	41.0
15	39.35977268968835	40.0	39.0	41.0	37.0	41.0
16	39.32296147708749	40.0	39.0	41.0	36.0	41.0
17	39.31416673288676	40.0	39.0	41.0	36.0	41.0
18	39.342885492215736	40.0	39.0	41.0	36.0	41.0
19	39.37472482866893	40.0	39.0	41.0	37.0	41.0
20	39.35239970877477	40.0	39.0	41.0	36.0	41.0
21	39.332935296884365	40.0	39.0	41.0	36.0	41.0
22	39.27931849860079	40.0	39.0	41.0	36.0	41.0
23	39.20964565449434	40.0	39.0	41.0	36.0	41.0
24	39.180486621035655	40.0	39.0	41.0	36.0	41.0
25	39.15121805596421	40.0	39.0	41.0	36.0	41.0
26	39.03552260539196	40.0	39.0	41.0	35.0	41.0
27	38.95399457496365	40.0	39.0	41.0	35.0	41.0
28	38.84672876321628	40.0	38.0	41.0	35.0	41.0
29	38.754397909020035	40.0	38.0	41.0	35.0	41.0
30	38.628069301295696	40.0	38.0	41.0	35.0	41.0
31	38.5030357438163	40.0	38.0	41.0	35.0	41.0
32	38.38856017473514	40.0	38.0	41.0	35.0	41.0
33	38.247801313949815	40.0	38.0	41.0	34.0	41.0
34	38.19298611096197	40.0	37.0	41.0	34.0	41.0
35	38.04095193709879	40.0	37.0	41.0	34.0	41.0
36	37.95498895019318	40.0	37.0	41.0	34.0	41.0
37	37.848689593852484	40.0	37.0	41.0	33.0	41.0
38	37.716300236888955	40.0	37.0	41.0	33.0	41.0
39	37.591668295323	40.0	36.0	41.0	33.0	41.0
40	37.44148327280459	40.0	36.0	41.0	33.0	41.0
41	37.29502662047726	40.0	36.0	41.0	33.0	41.0
42	37.10777910695225	40.0	35.0	41.0	32.0	41.0
43	36.18251832506835	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	1.0
14	2.0
15	1.0
16	1.0
17	5.0
18	13.0
19	22.0
20	44.0
21	98.0
22	184.0
23	312.0
24	484.0
25	693.0
26	1083.0
27	1545.0
28	2347.0
29	3090.0
30	4089.0
31	5202.0
32	6729.0
33	9102.0
34	13013.0
35	18563.0
36	30207.0
37	62163.0
38	124878.0
39	181740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15502589026651	19.15621999961342	13.126612101310297	24.56214200880978
2	19.654481453720745	20.34925550718506	33.757428283639626	26.238834755454565
3	19.31020856107676	23.98226876480556	29.387116934661172	27.32040573945651
4	13.709277327600464	17.283014653611644	35.28679027273371	33.72091774605418
5	13.787452831606958	37.80537306252537	33.36504738853011	15.042126717337565
6	30.799859971349967	36.11472040445085	17.369995640212277	15.715423983986906
7	29.325693324370356	30.60528028280633	21.355442969466452	18.713583423356862
8	29.325478556502205	31.243355619079118	18.868216288424655	20.562949535994022
9	27.75616974393227	13.889897104714368	20.331429774128633	38.02250337722472
10	19.761006316323	25.629538313513837	30.069434451772803	24.540020918390358
11	35.09092197698118	23.614371406665104	20.065976689095592	21.228729927258122
12	22.822951812533425	28.013461649975625	26.107396820146946	23.056189717344008
13	31.042547662359137	19.212274413200493	25.04214819412438	24.703029730315986
14	23.184406134629384	21.169883531385103	25.07114185632459	30.574568477660918
15	27.480193033359896	26.83653373251521	21.710239487649773	23.97303374647512
16	25.209881899149305	25.26421816979118	24.85787736325193	24.668022567807586
17	22.947517176060256	27.402876600825998	25.350340084919214	24.299266138194533
18	23.294582050990186	24.34028680101113	27.159329838344227	25.205801309654458
19	23.896146849677528	25.838722217091657	27.901138054933327	22.36399287829749
20	24.043692375096377	24.374005356310633	28.095073439872515	23.48722882872048
21	25.385776783163916	24.741043642978486	26.988804151033357	22.88437542282424
22	25.919045399779648	23.753326217357966	26.190082449384583	24.1375459334778
23	23.72304394794886	24.62979388727694	27.226981716811384	24.42018044796282
24	22.824025651874173	25.505832021459607	26.609953631617266	25.060188695048957
25	23.75117853867647	24.48632895135293	27.21087412670016	24.551618383270444
26	23.717674751245116	25.6228805096012	27.043355189543384	23.616089549610304
27	23.60277394178502	25.02732921122205	26.42138744338182	24.94850940361111
28	22.86375770748187	25.043866337069577	28.516662765050395	23.575713190398158
29	23.406905646032484	24.972992940580173	28.162295782603376	23.457805630783966
30	22.581337960865	26.023637351568556	28.190859909067285	23.20416477849916
31	23.5097794548762	25.924844132219686	27.26134457571534	23.304031837188774
32	22.57360631761161	25.178096254663146	28.07467049239829	24.173626935326954
33	21.867234799267212	25.049020765905173	28.551240391822496	24.53250404300512
34	24.156015970138675	24.168902042227657	27.555576555080442	24.119505432553225
35	23.27890399661526	25.33122574465389	28.078965849761282	23.310904408969567
36	22.400503415882945	25.504328646382557	27.408890101134187	24.686277836600308
37	22.83218683086386	23.885408456270042	28.58646232219905	24.695942390667046
38	21.94175924951516	23.73743339511489	29.60854260672352	24.712264748646426
39	21.928873177426176	23.77609161138184	30.02454796732951	24.27048724386247
40	21.702293076528235	23.548222903274997	30.52388326077759	24.225600759419184
41	20.431082064950097	23.77587684351369	30.8881295651595	24.904911526376715
42	20.36343018648294	24.351025194418614	30.579722906496514	24.70582171260193
43	19.68132743723946	23.557243153737282	31.249798655123612	25.51163075389965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	57.5
2	82.0
3	204.0
4	326.0
5	326.0
6	460.0
7	594.0
8	594.5
9	595.0
10	930.5
11	1266.0
12	1266.0
13	2353.5
14	3441.0
15	5446.0
16	7451.0
17	6624.5
18	5798.0
19	5798.0
20	6496.5
21	7195.0
22	5026.0
23	2857.0
24	2613.5
25	2370.0
26	2370.0
27	2760.0
28	3150.0
29	4867.5
30	6585.0
31	8274.0
32	9963.0
33	9963.0
34	12693.5
35	15424.0
36	18200.5
37	20977.0
38	22608.5
39	24240.0
40	24240.0
41	27360.0
42	30480.0
43	32538.0
44	34596.0
45	36059.5
46	37523.0
47	37523.0
48	40076.0
49	42629.0
50	41180.0
51	39731.0
52	36711.0
53	33691.0
54	33691.0
55	30151.0
56	26611.0
57	23173.5
58	19736.0
59	19311.5
60	18887.0
61	18887.0
62	18026.0
63	17165.0
64	15402.0
65	13639.0
66	12559.0
67	11479.0
68	11479.0
69	9990.5
70	8502.0
71	7592.5
72	6683.0
73	5233.5
74	3784.0
75	3784.0
76	3045.5
77	2307.0
78	1996.5
79	1686.0
80	1505.0
81	1324.0
82	1324.0
83	1191.0
84	1058.0
85	917.0
86	776.0
87	638.0
88	500.0
89	500.0
90	411.0
91	322.0
92	210.5
93	99.0
94	71.5
95	44.0
96	44.0
97	30.5
98	17.0
99	10.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	465619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.498745606382144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76686845715638	26.82859411508422
2	8.41938532231721	5.809164647934583
3	3.3323425150655686	3.448849101017362
4	1.907334356625719	2.6320257102217295
5	1.2737662188047716	2.197166837227456
6	1.0082208776734551	2.0869413344339915
7	0.7260211060156497	1.7532772208908673
8	0.5851058095177929	1.6148333180296504
9	0.4829380603763752	1.4994681559707772
>10	3.8374812975054233	27.087465181822868
>50	0.45122002356546653	10.737875291520908
>100	0.2049290940359827	11.33869990866617
>500	0.0018800834315227772	0.3926223478718232
>1k	0.002506777908697036	2.5730168293075812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4092	0.8788301164686149	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3623	0.7781039863064008	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2955	0.6346390503823942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1231	0.2643792456922935	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	751	0.1612906689804325	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	558	0.1198404704275384	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	507	0.10888730915190317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.147678681497104E-4	0.0
13	0.0	0.0	0.0	2.147678681497104E-4	0.0
14	0.0	0.0	0.0	2.147678681497104E-4	0.0
15	0.0	0.0	0.0	6.443036044491312E-4	0.0
16	0.0	0.0	0.0	8.590714725988416E-4	0.0
17	0.0	0.0	0.0	0.0012886072088982624	0.0
18	0.0	0.0	0.0	0.0012886072088982624	0.0
19	0.0	0.0	0.0	0.0012886072088982624	0.0
20	0.0	0.0	0.0	0.0015033750770479728	0.0
21	0.0	0.0	0.0	0.002791982285946235	0.0
22	0.0	0.0	0.0	0.004080589494844497	0.0
23	0.0	0.0	0.0	0.006228268176341601	0.0
24	0.0	0.0	0.0	0.011597464880084361	0.0
25	0.0	0.0	0.0	0.014174679297880886	0.0
26	0.0	0.0	0.0	0.020832483210521908	0.0
27	0.0	0.0	0.0	0.05970546734561949	0.0
28	0.0	0.0	0.0	0.18061977711390642	0.0
29	0.0	0.0	0.0	0.3464205713254829	0.0
30	0.0	0.0	0.0	0.5777255653227209	0.0
31	0.0	0.0	0.0	1.2142975265184626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCATA	20	0.0018408119	37.0	34
GTTGAAT	30	3.59622E-4	30.833332	10
GTTTCCA	25	0.0054930276	29.6	37
GAGTCAT	25	0.0054930276	29.6	9
GAGTTGG	25	0.0054930276	29.6	7
GTGACTC	25	0.0054930276	29.6	7
TATGCCG	35	8.861441E-4	26.42857	13
TCCAGGA	35	8.861441E-4	26.42857	2
GGTATTG	70	6.5701897E-9	26.42857	33
TGTACGG	35	8.861441E-4	26.42857	10
CCTGTTT	50	9.0759295E-6	25.900002	12
TAAGATA	50	9.0759295E-6	25.900002	4
TCTAGCG	45	1.3215611E-4	24.666666	28
CCAAGCA	45	1.3215611E-4	24.666666	36
CAGTATT	45	1.3215611E-4	24.666666	14
CTAGATA	40	0.0019294716	23.125	13
CGTATGC	40	0.0019294716	23.125	11
CTCGTAT	40	0.0019294716	23.125	9
TCTTCTG	40	0.0019294716	23.125	20
TACCTTA	40	0.0019294716	23.125	31
>>END_MODULE