FastQCFastQC Report
Fri 10 Feb 2017
ERR1631467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219536
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT4200.19131258654617012No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4060.18493550032796444No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG2730.12435318125501058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC250.005486781729.5999983
TATACTG250.005486781729.5999985
AATAACG250.005486781729.5999982
AACGCCG358.846457E-426.4285725
GTAGGAC400.001926240723.1253
GGTATCA2250.023.0222231
TATACAC903.785317E-922.6111137
ATACACA502.692464E-422.19999937
TACTCTT450.00381610220.5555555
GACAGGC1450.020.4137947
CAGGACA1550.020.2903234
AACAGCC656.869672E-519.9230777
TGCATCA1457.2759576E-1219.1379314
TCGCCGG609.204401E-418.517
CATCAGA1501.2732926E-1118.516
ACAATAG500.00701720818.4999985
ACAGCGG500.00701720818.4999988
TCAAGGG500.00701720818.4999983
GCATCAG1552.0008883E-1117.90322715
GGACAGG2100.017.6190476
Produced by FastQC (version 0.11.5)