##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631467.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219536
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777398695430364	31.0	31.0	34.0	30.0	34.0
2	32.00161249180089	33.0	31.0	34.0	30.0	34.0
3	32.094226003935574	33.0	31.0	34.0	30.0	34.0
4	35.85504883026019	37.0	35.0	37.0	35.0	37.0
5	35.35307193353254	37.0	35.0	37.0	33.0	37.0
6	35.31441768092704	37.0	35.0	37.0	32.0	37.0
7	35.30736189053276	37.0	35.0	37.0	32.0	37.0
8	35.27013337220319	37.0	35.0	37.0	32.0	37.0
9	36.9539164419503	38.0	37.0	39.0	33.0	39.0
10	36.74846949930763	38.0	37.0	39.0	33.0	39.0
11	36.84390714962466	38.0	37.0	39.0	33.0	39.0
12	36.586974345893154	38.0	35.0	39.0	32.0	39.0
13	36.73920905910648	38.0	37.0	39.0	32.0	39.0
14	37.87735496683915	39.0	37.0	40.0	33.0	41.0
15	37.81863566795423	39.0	37.0	40.0	33.0	41.0
16	37.77818672108447	39.0	37.0	40.0	33.0	41.0
17	37.73483164492384	39.0	37.0	40.0	33.0	41.0
18	37.68596949930763	39.0	37.0	40.0	33.0	41.0
19	37.685318125501055	39.0	37.0	40.0	33.0	41.0
20	37.654639785729906	39.0	37.0	40.0	33.0	41.0
21	37.624881568398806	39.0	37.0	40.0	33.0	41.0
22	37.55533944318927	39.0	37.0	40.0	32.0	41.0
23	37.485369142190805	39.0	37.0	40.0	32.0	41.0
24	37.414875009110126	39.0	36.0	40.0	32.0	41.0
25	37.36665057211574	39.0	36.0	40.0	32.0	41.0
26	37.21465727716639	39.0	36.0	40.0	32.0	41.0
27	37.1359139275563	39.0	36.0	40.0	31.0	41.0
28	37.034814335689816	39.0	36.0	40.0	31.0	41.0
29	36.897242365716785	39.0	36.0	40.0	31.0	41.0
30	36.810500327964434	39.0	36.0	40.0	31.0	41.0
31	36.7734357918519	38.0	36.0	40.0	31.0	41.0
32	36.663531265942716	38.0	35.0	40.0	30.0	41.0
33	36.5280910647912	38.0	35.0	40.0	30.0	41.0
34	36.46196523576999	38.0	35.0	40.0	30.0	41.0
35	36.34653086509729	38.0	35.0	40.0	30.0	41.0
36	36.293332300852704	38.0	35.0	40.0	30.0	41.0
37	36.22623624371401	38.0	35.0	40.0	30.0	41.0
38	36.14655455141754	38.0	35.0	40.0	30.0	41.0
39	36.053726951388384	38.0	35.0	40.0	29.0	41.0
40	35.92158916988558	38.0	34.0	40.0	29.0	41.0
41	35.78205396836965	38.0	34.0	40.0	29.0	41.0
42	35.68764120690912	38.0	34.0	40.0	29.0	41.0
43	34.61887344216894	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	4.0
17	8.0
18	26.0
19	37.0
20	81.0
21	122.0
22	196.0
23	349.0
24	510.0
25	796.0
26	1113.0
27	1532.0
28	2074.0
29	2678.0
30	3904.0
31	5250.0
32	7038.0
33	10061.0
34	14531.0
35	20925.0
36	30882.0
37	43490.0
38	49487.0
39	24439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00235041177757	16.564481451789227	11.689654544129437	27.74351359230377
2	21.30356752423293	18.553676845710953	31.9564900517455	28.18626557831062
3	21.90392464106115	19.765778733328474	27.79407477589097	30.536221849719407
4	16.647383572625902	14.531557466656949	30.69018293127323	38.13087602944392
5	17.94011005028788	33.55987172946578	30.831845346549088	17.66817287369725
6	38.150007288098536	31.020424896144593	14.592595291888347	16.236972523868523
7	32.84837110997741	27.68794184097369	18.21386925151228	21.249817797536622
8	30.52210115880767	30.464707382843812	17.73103272356242	21.282158734786094
9	28.549303986589898	13.217422199548137	16.903378033671014	41.32989578019095
10	19.90835216092122	24.136360323591575	29.086345747394503	26.868941768092704
11	39.55114423147001	19.66875592158006	18.1719626849355	22.60813716201443
12	25.371693025289705	22.838167772028278	25.199056191239706	26.59108301144231
13	33.34851687194811	16.88606879965017	21.978627651045844	27.786786677355877
14	25.080169083886013	18.13962174768603	21.694391808177247	35.08581736025071
15	29.116864660010204	23.86624517163472	19.06429924932585	27.952590919029223
16	28.375300634064573	22.71973617083303	20.438561329349174	28.466401865753227
17	26.859831644923837	23.62938196924422	21.95220829385613	27.558578091975804
18	27.053421762262225	21.506723270898622	22.999872458275636	28.439982508563517
19	28.084687704977775	22.470574302164565	22.37901756431747	27.065720428540196
20	28.163034764230012	21.845164346621967	22.299303986589898	27.692496902558123
21	30.064317469572188	21.637909044530282	21.29536841338095	27.002405072516584
22	30.113967640842503	21.679815611107063	20.776546898914074	27.429669849136364
23	28.553403542015886	22.020989723781064	21.05531666788135	28.370290066321697
24	28.265523649879746	21.91440128270534	21.75679615188397	28.063278915530937
25	27.861945193499015	21.354584213978573	21.97133955251075	28.812131040011664
26	28.281466365425263	21.95858538007434	22.398148822972086	27.361799431528315
27	28.83718387872604	21.607390131914585	21.29536841338095	28.260057575978426
28	27.690219371765906	21.35549522629546	22.616791779024854	28.337493622913783
29	27.38229720865826	21.594180453319726	23.374298520516	27.649223817506012
30	27.107626995116973	22.236444136724728	22.83179068581007	27.824138182348225
31	27.349956271408786	22.29383791268858	22.049231105604548	28.306974710298082
32	26.41890168355076	21.970884046352307	23.322370818453468	28.287843451643468
33	27.28254135995919	21.925788936666425	22.911504263537644	27.880165439836745
34	27.784509146563664	21.67526054952263	22.977097150353472	27.563133153560237
35	27.462921798702716	21.500801690838863	23.725493768675754	27.310782741782667
36	26.48495007652503	21.70987901756432	23.15656657678012	28.648604329130528
37	25.940620217185334	20.776546898914074	24.44291596822389	28.8399169156767
38	25.919211427738503	20.190310472997595	25.557539537934552	28.332938561329353
39	25.20497777129947	19.944337147438233	26.586983456016327	28.263701625245975
40	25.18857954959551	19.405473361999853	27.441057503097444	27.964889585307194
41	23.615716784490925	19.67194446468916	28.787989213614168	27.924349537205746
42	23.491363603235914	19.755757597842724	28.374389621747685	28.378489177173677
43	22.528879090445304	18.717659062750528	29.623387508199112	29.130074338605056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	2.5
2	0.0
3	6.5
4	13.0
5	13.0
6	17.5
7	22.0
8	24.5
9	27.0
10	43.0
11	59.0
12	59.0
13	98.5
14	138.0
15	266.5
16	395.0
17	386.0
18	377.0
19	377.0
20	414.5
21	452.0
22	439.5
23	427.0
24	510.5
25	594.0
26	594.0
27	777.0
28	960.0
29	1238.0
30	1516.0
31	1959.5
32	2403.0
33	2403.0
34	2993.5
35	3584.0
36	4395.5
37	5207.0
38	5987.0
39	6767.0
40	6767.0
41	7743.5
42	8720.0
43	9598.5
44	10477.0
45	11584.0
46	12691.0
47	12691.0
48	13735.0
49	14779.0
50	15400.5
51	16022.0
52	16989.5
53	17957.0
54	17957.0
55	17930.5
56	17904.0
57	17547.5
58	17191.0
59	16543.5
60	15896.0
61	15896.0
62	15402.5
63	14909.0
64	13958.0
65	13007.0
66	11417.0
67	9827.0
68	9827.0
69	8629.5
70	7432.0
71	6611.0
72	5790.0
73	5488.0
74	5186.0
75	5186.0
76	4732.0
77	4278.0
78	3400.5
79	2523.0
80	1805.5
81	1088.0
82	1088.0
83	763.5
84	439.0
85	350.5
86	262.0
87	194.0
88	126.0
89	126.0
90	97.5
91	69.0
92	39.5
93	10.0
94	6.5
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	219536.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.82435880389573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.47576214917315	67.53139486760384
2	4.490118578771779	6.629602500625724
3	1.3352834569190453	2.9572933508549353
4	0.7065262911176945	2.086354016794335
5	0.43153019374344603	1.5928719928815405
6	0.2882420526940719	1.2767570832275152
7	0.2106357742252536	1.0885035681339046
8	0.1738908811551667	1.0269906242499693
9	0.10577180585751558	0.7027682172265108
>10	0.7024709155524482	9.779684731935696
>50	0.06183558200809719	3.1320274216265527
>100	0.017932318782348185	2.195751624839487
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	420	0.19131258654617012	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	406	0.18493550032796444	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	273	0.12435318125501058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	4.5550615844326214E-4	0.0
18	0.0	0.0	0.0	9.110123168865243E-4	0.0
19	0.0	0.0	0.0	9.110123168865243E-4	0.0
20	0.0	0.0	0.0	9.110123168865243E-4	0.0
21	0.0	0.0	0.0	9.110123168865243E-4	0.0
22	0.0	0.0	0.0	0.0013665184753297865	0.0
23	0.0	0.0	0.0	0.0018220246337730486	0.0
24	0.0	0.0	0.0	0.00637708621820567	0.0
25	0.0	0.0	0.0	0.009565629327308506	0.0
26	0.0	0.0	0.0	0.012298666277968079	0.0
27	0.0	0.0	0.0	0.04281757889366664	0.0
28	0.0	0.0	0.0	0.16443772319801764	0.0
29	0.0	0.0	0.0	0.3521062604766416	0.0
30	0.0	0.0	0.0	0.5780373150644996	0.0
31	0.0	0.0	0.0	1.2453538371838788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	25	0.0054867817	29.599998	3
TATACTG	25	0.0054867817	29.599998	5
AATAACG	25	0.0054867817	29.599998	2
AACGCCG	35	8.846457E-4	26.428572	5
GTAGGAC	40	0.0019262407	23.125	3
GGTATCA	225	0.0	23.022223	1
TATACAC	90	3.785317E-9	22.61111	37
ATACACA	50	2.692464E-4	22.199999	37
TACTCTT	45	0.003816102	20.555555	5
GACAGGC	145	0.0	20.413794	7
CAGGACA	155	0.0	20.290323	4
AACAGCC	65	6.869672E-5	19.923077	7
TGCATCA	145	7.2759576E-12	19.13793	14
TCGCCGG	60	9.204401E-4	18.5	17
CATCAGA	150	1.2732926E-11	18.5	16
ACAATAG	50	0.007017208	18.499998	5
ACAGCGG	50	0.007017208	18.499998	8
TCAAGGG	50	0.007017208	18.499998	3
GCATCAG	155	2.0008883E-11	17.903227	15
GGACAGG	210	0.0	17.619047	6
>>END_MODULE