FastQCFastQC Report
Fri 10 Feb 2017
ERR1631466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390451
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.22922210469431503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7890.20207401184783752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6520.16698638241418257No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT4390.11243408263777016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATG250.00549195529.5999983
GATAACG803.274181E-1127.7511
CGAACGA856.91216E-1126.11764716
TCCGATA856.91216E-1126.1176478
CCGATAA856.91216E-1126.1176479
TAACGAA856.91216E-1126.11764713
ATAACGA856.91216E-1126.11764712
CGATAAC901.4188117E-1024.66666610
ACGAACG952.8012437E-1023.36842215
CTAGACA502.6976768E-422.1999994
TTCCGAT1005.311449E-1022.1999997
AATTCCG1107.4578566E-1121.8636365
CGCGTAA705.087699E-621.14285710
ATTCCGA1151.3460522E-1020.9130426
TATAGAG450.003821353420.5555553
AACGAGA1101.7444108E-920.18181818
CGAGACT1101.7444108E-920.18181820
GGTATCA4050.020.0987641
GGTTAAT1202.3646862E-1020.0416681
GTTAATT1205.142283E-918.52
Produced by FastQC (version 0.11.5)