##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390451
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.058995879124396	34.0	33.0	34.0	31.0	34.0
2	33.19620643819583	34.0	33.0	34.0	31.0	34.0
3	33.28423540982095	34.0	34.0	34.0	31.0	34.0
4	36.50474963567772	37.0	37.0	37.0	35.0	37.0
5	36.48317970756894	37.0	37.0	37.0	35.0	37.0
6	36.524857152369954	37.0	37.0	37.0	35.0	37.0
7	36.53883329790422	37.0	37.0	37.0	35.0	37.0
8	36.53522977274997	37.0	37.0	37.0	35.0	37.0
9	38.3885045754781	39.0	39.0	39.0	37.0	39.0
10	38.34871725261301	39.0	39.0	39.0	37.0	39.0
11	38.400401074654695	39.0	39.0	39.0	37.0	39.0
12	38.36729320708616	39.0	39.0	39.0	37.0	39.0
13	38.38904241505336	39.0	39.0	39.0	37.0	39.0
14	39.9396185436841	41.0	40.0	41.0	38.0	41.0
15	39.913395022678905	41.0	40.0	41.0	38.0	41.0
16	39.88105549736074	41.0	40.0	41.0	38.0	41.0
17	39.8704959136998	41.0	40.0	41.0	38.0	41.0
18	39.839193650419645	41.0	40.0	41.0	38.0	41.0
19	39.84236178163201	41.0	40.0	41.0	38.0	41.0
20	39.79098273534964	41.0	40.0	41.0	38.0	41.0
21	39.76327887494205	41.0	40.0	41.0	38.0	41.0
22	39.701073886351935	41.0	40.0	41.0	37.0	41.0
23	39.64146845570891	41.0	40.0	41.0	37.0	41.0
24	39.60652937244366	41.0	40.0	41.0	37.0	41.0
25	39.606816220217134	41.0	40.0	41.0	37.0	41.0
26	39.50206556008308	41.0	40.0	41.0	37.0	41.0
27	39.45931243613155	41.0	40.0	41.0	36.0	41.0
28	39.38427357081939	41.0	39.0	41.0	36.0	41.0
29	39.34115932601018	41.0	39.0	41.0	36.0	41.0
30	39.27701043152662	41.0	39.0	41.0	35.0	41.0
31	39.22922978811682	41.0	39.0	41.0	35.0	41.0
32	39.153184394456666	41.0	39.0	41.0	35.0	41.0
33	39.07031868275405	41.0	39.0	41.0	35.0	41.0
34	39.01593541827272	41.0	39.0	41.0	35.0	41.0
35	38.91634033463866	41.0	39.0	41.0	35.0	41.0
36	38.85957777032201	41.0	39.0	41.0	35.0	41.0
37	38.787845337827285	40.0	38.0	41.0	35.0	41.0
38	38.71655086041526	40.0	38.0	41.0	35.0	41.0
39	38.633859306289395	40.0	38.0	41.0	35.0	41.0
40	38.53731710253015	40.0	38.0	41.0	35.0	41.0
41	38.43950969519863	40.0	38.0	41.0	35.0	41.0
42	38.30707566378368	40.0	37.0	41.0	35.0	41.0
43	37.47241267150039	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	3.0
15	1.0
16	4.0
17	3.0
18	5.0
19	16.0
20	22.0
21	64.0
22	91.0
23	139.0
24	245.0
25	363.0
26	553.0
27	850.0
28	1157.0
29	1589.0
30	2138.0
31	2894.0
32	3588.0
33	4825.0
34	7167.0
35	10640.0
36	17135.0
37	32572.0
38	72082.0
39	232303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35050749005637	16.787253714294494	11.473398710721703	27.38884008492743
2	20.191009883442483	19.198311695961852	32.16920945265859	28.441468967937077
3	20.58132774663146	20.309078475916312	28.219930285746482	30.889663491705743
4	15.080765576218273	14.680715377857915	32.11901109230095	38.11950795362286
5	17.31356815579932	33.749945575757266	31.395744920617442	17.540741347825975
6	36.667597214503225	32.185088525832946	15.200114739109388	15.947199520554436
7	32.0490919475171	28.536743406983206	19.182432622787495	20.231732022712198
8	29.716148761304233	30.32774919260035	18.79365144409926	21.162450601996152
9	28.14027880579125	13.435232590004892	18.17667261705054	40.24781598715332
10	19.18576210587244	24.153350868610914	29.831656212943493	26.82923081257315
11	39.20594389564888	19.7988992216693	19.283597685753143	21.71155919692868
12	24.307787660935688	23.609108441264077	26.380006710188987	25.703097187611252
13	32.225810665102664	17.10790854678308	23.10814929402153	27.55813149409273
14	24.50345882069709	18.866131729717686	22.55238173291911	34.07802771666611
15	27.650076450053913	25.383210697373038	20.16719127368095	26.7995215788921
16	27.611403223451852	23.71155407464701	21.854214741414417	26.822827960486716
17	26.25860863463021	24.246832509072842	22.906331396257148	26.5882274600398
18	26.89095430668637	22.1172439051251	24.433795789996697	26.558005998191835
19	27.08918660728235	23.3532504718902	23.542774893648627	26.014788027178824
20	27.04359830042694	22.70605018299351	23.5645445907425	26.685806925837046
21	28.72754839915892	22.73499107442419	23.087404053261483	25.450056473155403
22	28.245541694092218	22.34211207040064	22.58618879193548	26.826157443571663
23	26.744969279115686	22.209701089253198	23.38398416190508	27.661345469726033
24	26.386153448191962	23.124028367195884	23.293063662277724	27.196754522334427
25	27.002876161157225	22.321622943724055	23.306381594617505	27.369119300501215
26	26.915541258698273	23.17422672755352	23.301771541115272	26.608460472632927
27	27.184717160411935	22.34979549290436	23.10405146868621	27.361435877997497
28	26.184591664511043	22.84281510355973	23.94794737367813	27.0246458582511
29	26.024520362350206	23.128382306614657	23.976119922858437	26.8709774081767
30	25.40882210571877	23.449805481353614	24.522155148789476	26.61921726413814
31	26.797472666224444	23.146054178373216	22.92067378493076	27.13579937047158
32	25.764308453557554	22.781859951696884	23.773021454676773	27.68081014006879
33	25.54379422770079	22.36106451257648	24.419453401323086	27.675687858399645
34	26.54494417993551	22.242739806019195	24.14797247285831	27.064343541186986
35	26.470158867565967	22.38027306883578	24.747023314065018	26.40254474953323
36	25.300741962499774	22.661998560638853	24.81412520393084	27.223134272930533
37	25.835252054675234	21.626017093053928	25.373734476285115	27.164996375985716
38	25.03515165795452	21.110459443054314	26.478098404153144	27.376290494838017
39	25.229798361382095	20.79543912040179	26.620497834555422	27.354264683660688
40	24.81310074759701	21.241333739701012	26.94729940504698	26.998266107654995
41	23.762264663171564	21.062309995364334	28.317765865627187	26.857659475836915
42	24.140801278521504	21.31868019290513	28.067798520172826	26.47272000840054
43	22.68248768731544	20.689151775766998	28.45248187352574	28.175878663391824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	48.0
2	47.0
3	98.5
4	150.0
5	150.0
6	180.5
7	211.0
8	203.5
9	196.0
10	285.5
11	375.0
12	375.0
13	627.5
14	880.0
15	1275.0
16	1670.0
17	1568.5
18	1467.0
19	1467.0
20	1690.0
21	1913.0
22	1622.5
23	1332.0
24	1499.0
25	1666.0
26	1666.0
27	1969.5
28	2273.0
29	2812.0
30	3351.0
31	4140.5
32	4930.0
33	4930.0
34	6140.5
35	7351.0
36	9188.0
37	11025.0
38	12936.0
39	14847.0
40	14847.0
41	16243.0
42	17639.0
43	18857.0
44	20075.0
45	21852.5
46	23630.0
47	23630.0
48	25901.0
49	28172.0
50	29439.5
51	30707.0
52	31253.5
53	31800.0
54	31800.0
55	31192.0
56	30584.0
57	29691.0
58	28798.0
59	28089.5
60	27381.0
61	27381.0
62	25575.5
63	23770.0
64	21925.5
65	20081.0
66	18280.0
67	16479.0
68	16479.0
69	14121.5
70	11764.0
71	10279.0
72	8794.0
73	6998.0
74	5202.0
75	5202.0
76	4290.5
77	3379.0
78	2971.0
79	2563.0
80	2340.5
81	2118.0
82	2118.0
83	1800.5
84	1483.0
85	1293.5
86	1104.0
87	924.0
88	744.0
89	744.0
90	534.0
91	324.0
92	207.0
93	90.0
94	59.0
95	28.0
96	28.0
97	16.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	390451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.92907425017137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.91690698678116	55.325295676165624
2	6.284416497551749	7.909450247868718
3	1.9996364121396655	3.775058047586499
4	0.971202863402643	2.4446758841217586
5	0.5991194737630072	1.885101692457794
6	0.39586814749576255	1.4946969628223181
7	0.2863490991631656	1.261377860589597
8	0.19682787854030226	0.9908956946533112
9	0.15631583540631613	0.8853129731485471
>10	1.0217695653761223	12.26568586533636
>50	0.11004407340328928	4.873384689818392
>100	0.06032045505011838	6.2899261161128885
>500	0.0012227119266915887	0.599138289318207
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.22922210469431503	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	789	0.20207401184783752	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.16698638241418257	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	439	0.11243408263777016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.561140834573352E-4	0.0
2	0.0	0.0	0.0	2.561140834573352E-4	0.0
3	0.0	0.0	0.0	2.561140834573352E-4	0.0
4	0.0	0.0	0.0	2.561140834573352E-4	0.0
5	0.0	0.0	0.0	2.561140834573352E-4	0.0
6	0.0	0.0	0.0	2.561140834573352E-4	0.0
7	0.0	0.0	0.0	2.561140834573352E-4	0.0
8	0.0	0.0	0.0	2.561140834573352E-4	0.0
9	0.0	0.0	0.0	2.561140834573352E-4	0.0
10	0.0	0.0	0.0	5.122281669146704E-4	0.0
11	0.0	0.0	0.0	5.122281669146704E-4	0.0
12	0.0	0.0	0.0	7.683422503720057E-4	0.0
13	0.0	0.0	0.0	7.683422503720057E-4	0.0
14	0.0	0.0	0.0	7.683422503720057E-4	0.0
15	0.0	0.0	0.0	0.001280570417286676	0.0
16	0.0	0.0	0.0	0.0015366845007440113	0.0
17	0.0	0.0	0.0	0.0015366845007440113	0.0
18	0.0	0.0	0.0	0.0015366845007440113	0.0
19	0.0	0.0	0.0	0.0015366845007440113	0.0
20	0.0	0.0	0.0	0.0017927985842013465	0.0
21	0.0	0.0	0.0	0.002305026751116017	0.0
22	0.0	0.0	0.0	0.002561140834573352	0.0
23	0.0	0.0	0.0	0.003329483084945358	0.0
24	0.0	0.0	0.0	0.006658966169890716	0.0
25	0.0	0.0	0.0	0.009476221087921404	0.0
26	0.0	0.0	0.0	0.01613518725781212	0.0
27	0.0	0.0	0.0	0.05916235327864444	0.0
28	0.0	0.0	0.0	0.2138552596868749	0.0
29	0.0	0.0	0.0	0.3990257420265283	0.0
30	0.0	0.0	0.0	0.6500175438147169	0.0
31	0.0	0.0	0.0	1.229347600595209	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATG	25	0.005491955	29.599998	3
GATAACG	80	3.274181E-11	27.75	11
CGAACGA	85	6.91216E-11	26.117647	16
TCCGATA	85	6.91216E-11	26.117647	8
CCGATAA	85	6.91216E-11	26.117647	9
TAACGAA	85	6.91216E-11	26.117647	13
ATAACGA	85	6.91216E-11	26.117647	12
CGATAAC	90	1.4188117E-10	24.666666	10
ACGAACG	95	2.8012437E-10	23.368422	15
CTAGACA	50	2.6976768E-4	22.199999	4
TTCCGAT	100	5.311449E-10	22.199999	7
AATTCCG	110	7.4578566E-11	21.863636	5
CGCGTAA	70	5.087699E-6	21.142857	10
ATTCCGA	115	1.3460522E-10	20.913042	6
TATAGAG	45	0.0038213534	20.555555	3
AACGAGA	110	1.7444108E-9	20.181818	18
CGAGACT	110	1.7444108E-9	20.181818	20
GGTATCA	405	0.0	20.098764	1
GGTTAAT	120	2.3646862E-10	20.041668	1
GTTAATT	120	5.142283E-9	18.5	2
>>END_MODULE