FastQCFastQC Report
Fri 10 Feb 2017
ERR1631465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491601
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.21562201866961217No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA10270.20890925771102986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8750.1779898739018025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8290.16863269195953628No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA5410.11004859632100016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAA250.005493321529.65
CGCTTAT1650.023.54545426
GAGTATA400.001929623323.1250021
GGTATCA8500.022.6352941
TATAAAA502.6990546E-422.22
CAGTCGG6650.021.14285910
AGTCGGT6800.020.67647211
TTCTATA450.0038227420.5555572
TCTAGCG909.4505594E-820.55555728
CTAGCGG909.4505594E-820.55555729
CCGCTTA1900.020.44736925
GCTTATT2000.020.3527
CGGTGAT7200.020.29861314
TGTGGCG555.1373406E-420.18181810
TCTATAC555.1373406E-420.1818183
GCAGTCG7000.020.0857149
TCGGTGA7300.020.02054813
GTGATTC6950.019.9640316
ACACCGT656.891865E-519.9230776
TATACAC2250.019.73333437
Produced by FastQC (version 0.11.5)