##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631465.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491601
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06145024115085	34.0	33.0	34.0	31.0	34.0
2	33.20121399264851	34.0	33.0	34.0	31.0	34.0
3	33.28121789825489	34.0	34.0	34.0	31.0	34.0
4	36.517169411779065	37.0	37.0	37.0	35.0	37.0
5	36.51097739833727	37.0	37.0	37.0	35.0	37.0
6	36.56067827364061	37.0	37.0	37.0	35.0	37.0
7	36.558670547863	37.0	37.0	37.0	35.0	37.0
8	36.55412824628103	37.0	37.0	37.0	35.0	37.0
9	38.40773920313425	39.0	39.0	39.0	37.0	39.0
10	38.36355296266688	39.0	39.0	39.0	37.0	39.0
11	38.42426683428227	39.0	39.0	39.0	37.0	39.0
12	38.38071728902097	39.0	39.0	39.0	37.0	39.0
13	38.40344507029074	39.0	39.0	39.0	37.0	39.0
14	39.95396469901404	41.0	40.0	41.0	38.0	41.0
15	39.95167625777816	41.0	40.0	41.0	38.0	41.0
16	39.92266695958715	41.0	40.0	41.0	38.0	41.0
17	39.881196336053016	41.0	40.0	41.0	38.0	41.0
18	39.883456298909074	41.0	40.0	41.0	38.0	41.0
19	39.90433501966025	41.0	40.0	41.0	38.0	41.0
20	39.89124513579102	41.0	40.0	41.0	38.0	41.0
21	39.861831037772504	41.0	40.0	41.0	38.0	41.0
22	39.81943690106407	41.0	40.0	41.0	38.0	41.0
23	39.76606638310337	41.0	40.0	41.0	38.0	41.0
24	39.74657700045362	41.0	40.0	41.0	38.0	41.0
25	39.71773247003159	41.0	40.0	41.0	37.0	41.0
26	39.637132552618894	41.0	40.0	41.0	37.0	41.0
27	39.58029580899957	41.0	40.0	41.0	37.0	41.0
28	39.51457177670509	41.0	40.0	41.0	37.0	41.0
29	39.45428304661707	41.0	39.0	41.0	36.0	41.0
30	39.37701916798379	41.0	39.0	41.0	36.0	41.0
31	39.320977784829566	41.0	39.0	41.0	36.0	41.0
32	39.249254985242096	41.0	39.0	41.0	35.0	41.0
33	39.17897237800574	41.0	39.0	41.0	35.0	41.0
34	39.12076053547491	41.0	39.0	41.0	35.0	41.0
35	39.03724768664018	41.0	39.0	41.0	35.0	41.0
36	38.97090323249953	41.0	39.0	41.0	35.0	41.0
37	38.916584791324674	41.0	39.0	41.0	35.0	41.0
38	38.84370048067437	40.0	38.0	41.0	35.0	41.0
39	38.757048907549006	40.0	38.0	41.0	35.0	41.0
40	38.6669697579948	40.0	38.0	41.0	35.0	41.0
41	38.57639223679366	40.0	38.0	41.0	35.0	41.0
42	38.45135180766516	40.0	38.0	41.0	35.0	41.0
43	37.6229014993867	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	4.0
17	5.0
18	4.0
19	16.0
20	25.0
21	58.0
22	108.0
23	156.0
24	230.0
25	374.0
26	573.0
27	824.0
28	1204.0
29	1703.0
30	2271.0
31	2924.0
32	3844.0
33	5258.0
34	8256.0
35	12427.0
36	20650.0
37	40514.0
38	95728.0
39	294441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35222873834675	16.01441006019109	11.951155510261371	27.68220569120079
2	20.05956049723251	18.63625175701433	32.88581593609452	28.418371809658645
3	19.9979251466128	20.55427063818015	28.11121214155382	31.336592073653225
4	14.478408302668221	15.160872333457418	32.82784209145221	37.53287727242215
5	16.700535596957693	34.71005958083893	31.902904998159077	16.686499824044297
6	35.92100097436742	32.632968606654586	16.160666882288684	15.285363536689307
7	32.54000703822816	28.686068580006957	19.09556734018035	19.678357041584537
8	30.81950606284365	30.615682230101243	18.032306687740668	20.532505019314446
9	28.95173118036782	13.553674626373827	17.13177963429692	40.36281455896143
10	18.866316382594828	24.28127688918452	29.30771092817142	27.54469580004923
11	38.73059656103222	21.0245707392784	18.660458379864973	21.58437431982441
12	24.958452078006353	23.873018972703473	26.213128126265	24.955400823025176
13	32.75827347788145	17.182430466984407	23.028024759917088	27.031271295217056
14	24.438314812215598	18.366520816678566	22.388888549860557	34.80627582124528
15	28.673456726084773	24.561178679457527	19.49772274669905	27.26764184775865
16	27.690138954151845	23.62546048523091	21.79124940754799	26.893151153069255
17	25.83945109957059	24.55507616949518	22.922858171565967	26.682614559368268
18	27.168577769369872	22.30406366138393	23.804670861125178	26.72268770812102
19	27.111621009721297	24.16919412287607	23.045722038807895	25.673462828594733
20	27.609382405650113	22.218425104912317	23.35450904290268	26.817683446534897
21	29.14416366118051	22.238156553790574	22.737545285709345	25.880134499319567
22	28.962919115298792	21.842307074232966	21.800606589490258	27.394167220977987
23	27.14579506551044	22.324201944259674	22.48205353528573	28.047949454944153
24	26.585788067965687	22.909636066647547	23.373019989788464	27.131555875598302
25	26.53228939729577	22.09149289769549	22.904143807681432	28.472073897327306
26	27.532694197123277	22.710897658873762	22.66695958714486	27.089448556858102
27	27.332735287357025	22.12017469451852	22.386854379873107	28.160235638251347
28	26.351248268412796	22.63278553135571	23.365900394832394	27.650065805399095
29	25.972892650747255	22.93994519946054	24.173059045852224	26.914103103939986
30	25.451331465965282	23.571758397562252	24.49344081887547	26.483469317597
31	26.521101462364804	23.449504781316556	22.611630163486243	27.4177635928324
32	25.368947581473595	23.03066918090077	24.17529663283842	27.425086604787214
33	25.842298937553014	22.15068724433026	24.576231537364652	27.430782280752076
34	26.88908281309436	22.14031297739427	23.83904833391307	27.131555875598302
35	26.229401486164594	22.502598652158966	24.27924271919707	26.98875714247937
36	24.914920840274938	22.743444378672947	24.895799642392916	27.4458351386592
37	25.3345701086857	21.732665311909454	25.3585733145376	27.57419126486724
38	24.72594644844091	20.562814152127437	26.855925842298937	27.855313557132718
39	25.10491231710269	20.14682638969408	27.278829782689616	27.46943151051361
40	24.241203740431775	20.49548312554287	27.17834178530963	28.084971348715726
41	22.714965998848662	20.66676023848609	29.027402303900928	27.590871458764322
42	22.89193878775674	20.71883499016479	28.92894847650839	27.46027774557009
43	21.619158626609792	20.571561083073465	29.546725901696703	28.262554388620035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	41.0
4	77.0
5	77.0
6	111.0
7	145.0
8	148.5
9	152.0
10	234.0
11	316.0
12	316.0
13	583.5
14	851.0
15	1396.5
16	1942.0
17	1829.5
18	1717.0
19	1717.0
20	1934.0
21	2151.0
22	1736.5
23	1322.0
24	1470.5
25	1619.0
26	1619.0
27	2015.5
28	2412.0
29	3122.5
30	3833.0
31	4804.5
32	5776.0
33	5776.0
34	7294.0
35	8812.0
36	10803.0
37	12794.0
38	14995.5
39	17197.0
40	17197.0
41	19203.0
42	21209.0
43	23480.0
44	25751.0
45	28516.0
46	31281.0
47	31281.0
48	35788.5
49	40296.0
50	41210.5
51	42125.0
52	41063.5
53	40002.0
54	40002.0
55	38540.0
56	37078.0
57	36473.0
58	35868.0
59	35106.5
60	34345.0
61	34345.0
62	32339.0
63	30333.0
64	28282.0
65	26231.0
66	23469.5
67	20708.0
68	20708.0
69	17976.5
70	15245.0
71	13040.0
72	10835.0
73	8695.5
74	6556.0
75	6556.0
76	5457.5
77	4359.0
78	3707.5
79	3056.0
80	2641.0
81	2226.0
82	2226.0
83	1846.5
84	1467.0
85	1117.5
86	768.0
87	611.5
88	455.0
89	455.0
90	325.0
91	195.0
92	124.5
93	54.0
94	33.5
95	13.0
96	13.0
97	12.5
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	491601.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.13817518758604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.19247922418666	63.25935908428718
2	5.690988806103901	7.983111397462131
3	1.5309323698238095	3.221304082651459
4	0.706593544193485	1.9823672715623988
5	0.41086972550565365	1.4408826393395462
6	0.2696255254168096	1.1346625402037478
7	0.17399139368729832	0.8542407196100386
8	0.14619331912615885	0.8202986102500162
9	0.094499084873506	0.5965194032932074
>10	0.6717553371692833	9.103966237719563
>50	0.06840738361150885	3.3740951551356027
>100	0.0422088100918852	5.344733223514394
>500	8.732857260390012E-4	0.458359159859034
>1k	5.821904840260007E-4	0.4261004751117166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1060	0.21562201866961217	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1027	0.20890925771102986	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	875	0.1779898739018025	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.16863269195953628	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	541	0.11004859632100016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.034169987449171E-4	0.0
13	0.0	0.0	0.0	2.034169987449171E-4	0.0
14	0.0	0.0	0.0	4.068339974898342E-4	0.0
15	0.0	0.0	0.0	4.068339974898342E-4	0.0
16	0.0	0.0	0.0	8.136679949796684E-4	0.0
17	0.0	0.0	0.0	0.0012205019924695028	0.0
18	0.0	0.0	0.0	0.0012205019924695028	0.0
19	0.0	0.0	0.0	0.0016273359899593369	0.0
20	0.0	0.0	0.0	0.002034169987449171	0.0
21	0.0	0.0	0.0	0.0038649229761534253	0.0
22	0.0	0.0	0.0	0.006712760958582265	0.0
23	0.0	0.0	0.0	0.009967432938500938	0.0
24	0.0	2.034169987449171E-4	0.0	0.01566310890335862	0.0
25	0.0	2.034169987449171E-4	0.0	0.016680193897083202	0.0
26	0.0	2.034169987449171E-4	0.0	0.022782703859430715	0.0
27	0.0	2.034169987449171E-4	0.0	0.05614309165359713	0.0
28	0.0	2.034169987449171E-4	0.0	0.21440151667714263	0.0
29	0.0	2.034169987449171E-4	0.0	0.4513823202149711	0.0
30	0.0	2.034169987449171E-4	0.0	0.7347421994666407	0.0
31	0.0	2.034169987449171E-4	0.0	1.612283132052213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATAA	25	0.0054933215	29.6	5
CGCTTAT	165	0.0	23.545454	26
GAGTATA	40	0.0019296233	23.125002	1
GGTATCA	850	0.0	22.635294	1
TATAAAA	50	2.6990546E-4	22.2	2
CAGTCGG	665	0.0	21.142859	10
AGTCGGT	680	0.0	20.676472	11
TTCTATA	45	0.00382274	20.555557	2
TCTAGCG	90	9.4505594E-8	20.555557	28
CTAGCGG	90	9.4505594E-8	20.555557	29
CCGCTTA	190	0.0	20.447369	25
GCTTATT	200	0.0	20.35	27
CGGTGAT	720	0.0	20.298613	14
TGTGGCG	55	5.1373406E-4	20.181818	10
TCTATAC	55	5.1373406E-4	20.181818	3
GCAGTCG	700	0.0	20.085714	9
TCGGTGA	730	0.0	20.020548	13
GTGATTC	695	0.0	19.96403	16
ACACCGT	65	6.891865E-5	19.923077	6
TATACAC	225	0.0	19.733334	37
>>END_MODULE