FastQCFastQC Report
Fri 10 Feb 2017
ERR1631464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175677
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2010.11441452210591027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAATG601.2551027E-930.83333433
ACTCACA303.5871845E-430.8333348
CGGACTA303.5871845E-430.83333424
GCGAAAG502.6986527E-729.59999818
GGACTGA250.00548383329.5999986
CTAAGGC250.00548383329.59999833
GAGCGAA502.6986527E-729.59999816
ACGAGTA250.00548383329.5999985
CTAATCT250.00548383329.59999828
ATACACA604.2791726E-827.75000237
GAATGTT556.211121E-726.9090935
AGCGAAA556.211121E-726.9090917
AATGTTT556.211121E-726.9090936
CGAAAGC601.3266599E-624.66666819
CATTTGC601.3266599E-624.66666825
GGTATCA751.3569661E-824.6666661
ACGGCGG400.001924715723.12520
ACGGAAT400.001924715723.12518
AGACGGA652.6614216E-622.769236
CAGAGCG652.6614216E-622.7692314
Produced by FastQC (version 0.11.5)