##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631464.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175677
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07432959351537	34.0	33.0	34.0	31.0	34.0
2	33.22901119668482	34.0	33.0	34.0	31.0	34.0
3	33.31855052169607	34.0	34.0	34.0	31.0	34.0
4	36.543844669478645	37.0	37.0	37.0	35.0	37.0
5	36.522248216898056	37.0	37.0	37.0	35.0	37.0
6	36.570154317298226	37.0	37.0	37.0	35.0	37.0
7	36.566175424216034	37.0	37.0	37.0	35.0	37.0
8	36.5511079993397	37.0	37.0	37.0	35.0	37.0
9	38.41942314588705	39.0	39.0	39.0	37.0	39.0
10	38.36092943299351	39.0	39.0	39.0	37.0	39.0
11	38.43396119013872	39.0	39.0	39.0	37.0	39.0
12	38.3778753052477	39.0	39.0	39.0	37.0	39.0
13	38.40324572937835	39.0	39.0	39.0	37.0	39.0
14	39.967707781895186	41.0	40.0	41.0	38.0	41.0
15	39.97730494031661	41.0	40.0	41.0	38.0	41.0
16	39.95292497025792	41.0	40.0	41.0	38.0	41.0
17	39.92624532522755	41.0	40.0	41.0	38.0	41.0
18	39.8980913836188	41.0	40.0	41.0	38.0	41.0
19	39.914166339361444	41.0	40.0	41.0	38.0	41.0
20	39.87543047752409	41.0	40.0	41.0	38.0	41.0
21	39.842682878236765	41.0	40.0	41.0	38.0	41.0
22	39.79942166589821	41.0	40.0	41.0	38.0	41.0
23	39.745897300158816	41.0	40.0	41.0	38.0	41.0
24	39.71995195728525	41.0	40.0	41.0	37.0	41.0
25	39.68520068079487	41.0	40.0	41.0	37.0	41.0
26	39.61109877787075	41.0	40.0	41.0	37.0	41.0
27	39.551392612578766	41.0	40.0	41.0	37.0	41.0
28	39.48238528663399	41.0	40.0	41.0	37.0	41.0
29	39.42772246793832	41.0	39.0	41.0	37.0	41.0
30	39.359124985057804	41.0	39.0	41.0	36.0	41.0
31	39.31668345884777	41.0	39.0	41.0	36.0	41.0
32	39.26330139972791	41.0	39.0	41.0	35.0	41.0
33	39.192973468353856	41.0	39.0	41.0	35.0	41.0
34	39.14395168405654	41.0	39.0	41.0	35.0	41.0
35	39.06241568332793	41.0	39.0	41.0	35.0	41.0
36	38.98952054053746	40.0	39.0	41.0	35.0	41.0
37	38.94364657866425	40.0	39.0	41.0	35.0	41.0
38	38.875071864842866	40.0	38.0	41.0	35.0	41.0
39	38.80705499297005	40.0	38.0	41.0	35.0	41.0
40	38.731723560853155	40.0	38.0	41.0	35.0	41.0
41	38.653904609026796	40.0	38.0	41.0	35.0	41.0
42	38.5461841903038	40.0	38.0	41.0	35.0	41.0
43	37.698543349442446	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	1.0
20	4.0
21	18.0
22	40.0
23	54.0
24	109.0
25	134.0
26	193.0
27	305.0
28	411.0
29	530.0
30	712.0
31	987.0
32	1255.0
33	1849.0
34	2950.0
35	4352.0
36	7437.0
37	14211.0
38	35779.0
39	104340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.126305663234234	16.876995850338975	11.862110577935644	28.13458790849115
2	20.815473852581725	18.374061487844166	31.90229796729225	28.908166692281856
3	20.35041581994228	19.627498192705932	27.412239507732945	32.609846479618845
4	15.537605947278244	14.563090216704518	31.648422958042318	38.25088087797492
5	17.921526437723777	33.197857431536285	31.664361299430205	17.216254831309733
6	37.823391792892636	32.427124780136275	14.32970736066759	15.4197760663035
7	33.233149473180895	27.2534253203322	19.223347393227343	20.29007781325956
8	29.53374659175646	31.001781678876572	17.938034005589802	21.526437723777157
9	27.915435714407693	13.660297022376291	17.379622830535585	41.044644432680435
10	19.287669985257033	23.749836347387536	29.59066923957035	27.371824427785086
11	40.46744878384763	19.396961469059697	18.84139642639617	21.294193320696504
12	25.096626194664072	22.84988928545	25.520130694399377	26.533353825486543
13	33.054412359045294	16.27190810407737	22.051264536621183	28.62241500025615
14	24.799489973075588	17.98072599145022	21.49569949395766	35.72408454151653
15	28.42944722416708	24.353785640692863	19.13113270376885	28.085634431371208
16	28.755044769662508	22.83053558519328	20.673167233047014	27.7412524120972
17	27.911451129060723	22.95007314560244	21.593037221719406	27.545438503617437
18	27.797605833432947	21.30785475617184	22.753690010644533	28.140849399750678
19	28.301940493063977	22.297739601655312	21.882204272613944	27.518115632666767
20	28.167603044223206	21.6061294307166	21.841219966187946	28.38504755887225
21	29.366963233661775	22.34213926695014	21.323223871081588	26.967673628306493
22	29.649299566818648	21.55603750064038	20.925903789340666	27.868759143200307
23	27.910312676104443	21.508791702954856	21.891881122742305	28.6890144981984
24	27.699698879193065	22.105341052044377	21.623775451538904	28.571184617223654
25	28.119788020059538	21.05227206748749	21.996618794719854	28.83132111773311
26	28.060588466333098	22.306847225305532	21.984665038678937	27.64789926968243
27	28.05660388098613	21.882204272613944	21.588483409894295	28.472708436505634
28	27.530638615185826	21.857158307575837	22.364908326075696	28.247294751162645
29	26.7274600545319	22.184463532505678	23.193132851767732	27.89494356119469
30	26.767305908001614	22.774182163857535	22.588045105506126	27.87046682263472
31	28.11523420823443	22.186740438418234	21.47236120835397	28.225664144993367
32	26.58629188795346	21.70062102608765	22.598860408590767	29.114226677368126
33	26.325586160965862	21.45756131992236	23.536945644563602	28.679906874548177
34	27.884697484588195	20.697643971606983	23.32860875356478	28.089049790240043
35	27.420777904905023	20.91451925977789	24.083972290055044	27.580730545262043
36	26.242479095157588	21.451869055140968	24.048111021932296	28.257540827769144
37	26.191817938603236	20.003756894755718	25.014657581812077	28.789767584828972
38	25.229825190548567	19.601313774711546	26.542461449136766	28.626399585603124
39	25.10231845944546	19.017287408141076	27.485669723412855	28.39472440900061
40	24.7249213044394	19.52332974720652	27.987158250653188	27.764590697700893
41	23.520438076697577	19.43965345492011	29.289548432634895	27.750360035747423
42	23.465223108318106	19.909265299384664	29.29524069741628	27.330270894880947
43	22.053541442533742	18.929626530507694	29.855928778383056	29.160903248575508
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	5.5
4	11.0
5	11.0
6	10.5
7	10.0
8	8.0
9	6.0
10	11.0
11	16.0
12	16.0
13	26.5
14	37.0
15	57.5
16	78.0
17	76.5
18	75.0
19	75.0
20	92.5
21	110.0
22	113.5
23	117.0
24	145.0
25	173.0
26	173.0
27	267.0
28	361.0
29	540.0
30	719.0
31	990.0
32	1261.0
33	1261.0
34	1749.0
35	2237.0
36	3022.5
37	3808.0
38	4693.0
39	5578.0
40	5578.0
41	6263.0
42	6948.0
43	8030.0
44	9112.0
45	9852.5
46	10593.0
47	10593.0
48	11741.0
49	12889.0
50	13645.0
51	14401.0
52	15061.0
53	15721.0
54	15721.0
55	15546.0
56	15371.0
57	15001.0
58	14631.0
59	14541.0
60	14451.0
61	14451.0
62	13507.5
63	12564.0
64	11572.5
65	10581.0
66	9314.5
67	8048.0
68	8048.0
69	6787.5
70	5527.0
71	4771.5
72	4016.0
73	3153.5
74	2291.0
75	2291.0
76	1841.0
77	1391.0
78	1178.0
79	965.0
80	824.5
81	684.0
82	684.0
83	539.5
84	395.0
85	328.0
86	261.0
87	201.5
88	142.0
89	142.0
90	107.0
91	72.0
92	43.0
93	14.0
94	12.0
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	175677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.98259906579422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71225916094193	56.34787971144731
2	6.851491379904942	8.904554346862161
3	2.0717297442799114	4.038791500356656
4	1.0336213206503722	2.686695994627195
5	0.6713130894016827	2.181183466810461
6	0.46073603310426653	1.7963894948627424
7	0.36398505264016845	1.6556886319160653
8	0.2842050097714796	1.477470416197615
9	0.1944557253057383	1.1372614590231906
>10	1.2694463504591462	15.201064598117581
>50	0.0717990070678836	3.2562600833767212
>100	0.01495812647247575	1.316760296402288
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	201	0.11441452210591027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	5.692264781388571E-4	0.0
21	0.0	0.0	0.0	5.692264781388571E-4	0.0
22	0.0	0.0	0.0	0.0011384529562777142	0.0
23	0.0	0.0	0.0	0.0011384529562777142	0.0
24	0.0	0.0	0.0	0.0028461323906942854	0.0
25	0.0	0.0	0.0	0.005692264781388571	0.0
26	0.0	0.0	0.0	0.010246076606499429	0.0
27	0.0	0.0	0.0	0.03927662699158114	0.0
28	0.0	0.0	0.0	0.19467545552348914	0.0
29	0.0	0.0	0.0	0.4434274264701697	0.0
30	0.0	0.0	0.0	0.7246253066707651	0.0
31	0.0	0.0	0.0	1.6456337482994359	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAATG	60	1.2551027E-9	30.833334	33
ACTCACA	30	3.5871845E-4	30.833334	8
CGGACTA	30	3.5871845E-4	30.833334	24
GCGAAAG	50	2.6986527E-7	29.599998	18
GGACTGA	25	0.005483833	29.599998	6
CTAAGGC	25	0.005483833	29.599998	33
GAGCGAA	50	2.6986527E-7	29.599998	16
ACGAGTA	25	0.005483833	29.599998	5
CTAATCT	25	0.005483833	29.599998	28
ATACACA	60	4.2791726E-8	27.750002	37
GAATGTT	55	6.211121E-7	26.90909	35
AGCGAAA	55	6.211121E-7	26.90909	17
AATGTTT	55	6.211121E-7	26.90909	36
CGAAAGC	60	1.3266599E-6	24.666668	19
CATTTGC	60	1.3266599E-6	24.666668	25
GGTATCA	75	1.3569661E-8	24.666666	1
ACGGCGG	40	0.0019247157	23.125	20
ACGGAAT	40	0.0019247157	23.125	18
AGACGGA	65	2.6614216E-6	22.76923	6
CAGAGCG	65	2.6614216E-6	22.76923	14
>>END_MODULE