FastQCFastQC Report
Fri 10 Feb 2017
ERR1631463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353463
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.28461253370225453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8530.241326532055689No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7390.20907421710334603No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4940.13976003146015284No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3790.10722480146436826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAT303.5944808E-430.8333341
TATACTG250.005491257629.65
TGTAACA400.00192855723.1252
GCGGTAA652.6741254E-622.7692334
TCTAGCG855.155198E-821.76470828
CGTTTAT603.716896E-521.58333431
TAAGCAG806.932096E-720.81255
ACGCCGT450.003820646320.55555524
CTAAGCA450.003820646320.5555554
TCTAAGC450.003820646320.5555553
TAGGAGT450.003820646320.5555554
CAGTACT450.003820646320.5555554
TATCTAG555.1334157E-420.1818181
TCGTTTA656.884859E-519.92307730
CTATACT656.884859E-519.9230774
CGCGGTA759.236033E-619.73333433
TTAGCTC759.236033E-619.7333344
CTAGCGG851.2403216E-619.58823629
GAATTTC1052.24627E-819.3809518
GCAGTCG1355.4569682E-1119.1851869
Produced by FastQC (version 0.11.5)