##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631463.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353463
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.085434684818495	34.0	33.0	34.0	31.0	34.0
2	33.216195188746774	34.0	33.0	34.0	31.0	34.0
3	33.30030017286109	34.0	34.0	34.0	31.0	34.0
4	36.51199701241714	37.0	37.0	37.0	35.0	37.0
5	36.49761078245814	37.0	37.0	37.0	35.0	37.0
6	36.544062603440814	37.0	37.0	37.0	35.0	37.0
7	36.559331528335356	37.0	37.0	37.0	35.0	37.0
8	36.558307375878094	37.0	37.0	37.0	35.0	37.0
9	38.41357369795424	39.0	39.0	39.0	37.0	39.0
10	38.37481433700274	39.0	39.0	39.0	37.0	39.0
11	38.42955839790869	39.0	39.0	39.0	37.0	39.0
12	38.40751648687416	39.0	39.0	39.0	37.0	39.0
13	38.42835600897406	39.0	39.0	39.0	37.0	39.0
14	39.99173605158107	41.0	40.0	41.0	38.0	41.0
15	39.9636737084221	41.0	40.0	41.0	38.0	41.0
16	39.93917043650962	41.0	40.0	41.0	38.0	41.0
17	39.93684204570209	41.0	40.0	41.0	38.0	41.0
18	39.91481428041973	41.0	40.0	41.0	38.0	41.0
19	39.91600818190306	41.0	40.0	41.0	38.0	41.0
20	39.87611716077779	41.0	40.0	41.0	38.0	41.0
21	39.852971881073834	41.0	40.0	41.0	38.0	41.0
22	39.80109092040751	41.0	40.0	41.0	38.0	41.0
23	39.75034444906539	41.0	40.0	41.0	38.0	41.0
24	39.72361746491146	41.0	40.0	41.0	38.0	41.0
25	39.72256785010029	41.0	40.0	41.0	37.0	41.0
26	39.631729487952065	41.0	40.0	41.0	37.0	41.0
27	39.59538339232112	41.0	40.0	41.0	37.0	41.0
28	39.53837601106763	41.0	40.0	41.0	37.0	41.0
29	39.50431021068683	41.0	40.0	41.0	37.0	41.0
30	39.44295442521565	41.0	39.0	41.0	36.0	41.0
31	39.40204773908443	41.0	39.0	41.0	36.0	41.0
32	39.332821257104705	41.0	39.0	41.0	36.0	41.0
33	39.27141171777527	41.0	39.0	41.0	35.0	41.0
34	39.206709047340176	41.0	39.0	41.0	35.0	41.0
35	39.125665203995894	41.0	39.0	41.0	35.0	41.0
36	39.062759044086654	41.0	39.0	41.0	35.0	41.0
37	39.006334467822654	41.0	39.0	41.0	35.0	41.0
38	38.927129572260746	41.0	39.0	41.0	35.0	41.0
39	38.84084897146236	41.0	39.0	41.0	35.0	41.0
40	38.764705216670485	40.0	38.0	41.0	35.0	41.0
41	38.67112257860087	40.0	38.0	41.0	35.0	41.0
42	38.543530723159144	40.0	38.0	41.0	35.0	41.0
43	37.750061534021945	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	2.0
17	0.0
18	2.0
19	10.0
20	14.0
21	42.0
22	75.0
23	122.0
24	186.0
25	276.0
26	472.0
27	652.0
28	962.0
29	1322.0
30	1763.0
31	2283.0
32	2916.0
33	3871.0
34	5563.0
35	8163.0
36	13395.0
37	27037.0
38	63817.0
39	220517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12937139106498	16.990180018842143	11.747481348825762	27.13296724126712
2	19.393543312878574	19.17767913473263	33.66010020850839	27.768677343880405
3	20.1797642186028	20.96796552963111	28.27028571590237	30.581984535863725
4	14.814846249819642	14.757131580957555	32.589832599168794	37.83818957005401
5	16.394360937354122	34.88342485634989	32.15159719687775	16.570617009418243
6	36.225573822436864	32.6150120380351	15.532601715030994	15.626812424497047
7	31.7623060971021	28.75831416583914	19.760201209178895	19.719178527879862
8	29.6257316890311	31.257585659602277	18.771130217307043	20.345552434059577
9	28.34978484310946	13.383579045048563	18.452002048304912	39.814634063537056
10	18.518204168470252	25.204901220212584	30.47645722466001	25.80043738665716
11	38.304716476689215	20.776432045221142	19.365534723577856	21.553316754511787
12	23.819749167522485	24.089084288878894	27.28800468507312	24.803161858525502
13	31.942240064730964	17.86608499333735	23.79626721891683	26.39540772301486
14	24.270998661811845	18.914285229288495	23.195921496733746	33.618794612165914
15	27.05884349988542	26.02620359132356	20.56254827237929	26.35240463641173
16	27.30780873811403	23.904340765511524	22.315772796586913	26.472077699787533
17	25.270820425334477	25.260069653683697	23.55267736651361	25.916432554468216
18	26.50942248552182	22.716380498100225	24.821551336349206	25.952645680028745
19	26.342219694847834	23.833329089607684	24.123034094091885	25.701417121452597
20	26.40898764510005	23.28164475489655	24.503837742564286	25.805529857439108
21	27.94606507611829	23.081906734226777	24.137179846263965	24.834848343390963
22	27.436817997923402	22.707044301666652	23.336530273324225	26.519607427085724
23	26.03808602314811	22.81285452791381	24.079748092445318	27.069311356492758
24	25.470558446004247	23.70064193423357	24.163490945304034	26.66530867445815
25	26.54337229073482	23.20893558873206	23.94734385211465	26.300348268418478
26	26.697843904453933	23.634439814068234	23.69045699266967	25.977259288808163
27	26.87409997651805	22.874812922427523	23.85992310369119	26.391163997363233
28	25.292887798722923	23.447715885396775	24.456025100222654	26.803371215657652
29	25.33447631010884	23.406693204097742	24.67726466419399	26.581565821599433
30	24.812215139915637	23.846060266562557	25.569012881121928	25.772711712399882
31	26.35523378684615	23.587193001813485	23.70460274484175	26.352970466498615
32	25.27874204655084	23.385757490883062	24.697351632278345	26.638148830287754
33	25.448491072615802	22.792201729742576	24.68490337036691	27.074403827274708
34	26.214059180168785	22.649895462891447	24.474414578046357	26.661630778893407
35	25.532233925474518	22.85302846408252	25.40548798601268	26.20924962443028
36	24.4746974930898	23.14924051456588	25.60098228103083	26.775079711313488
37	25.728576965622995	22.14376045017442	25.828163060914438	26.29949952328815
38	24.583619784814818	21.66818026214908	26.805351620961744	26.94284833207436
39	25.131343308917764	21.54482930320854	27.018669563716713	26.305157824156982
40	24.685752115497237	22.100757363571294	26.554405977429035	26.65908454350243
41	23.689042417452463	21.949680730373476	28.12854527913813	26.232731573035935
42	23.94734385211465	21.871596178383594	27.7352933687543	26.44576660074746
43	22.50051631995428	21.895643957076132	27.90900320542744	27.69483651754215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	45.0
2	45.0
3	119.5
4	194.0
5	194.0
6	227.0
7	260.0
8	231.5
9	203.0
10	292.5
11	382.0
12	382.0
13	699.0
14	1016.0
15	1473.0
16	1930.0
17	1777.5
18	1625.0
19	1625.0
20	1822.0
21	2019.0
22	1659.5
23	1300.0
24	1433.0
25	1566.0
26	1566.0
27	1829.5
28	2093.0
29	2667.0
30	3241.0
31	3995.5
32	4750.0
33	4750.0
34	5856.5
35	6963.0
36	8520.5
37	10078.0
38	11762.0
39	13446.0
40	13446.0
41	14895.0
42	16344.0
43	17608.0
44	18872.0
45	20909.0
46	22946.0
47	22946.0
48	25399.0
49	27852.0
50	28489.5
51	29127.0
52	29788.0
53	30449.0
54	30449.0
55	29849.5
56	29250.0
57	27759.0
58	26268.0
59	25272.5
60	24277.0
61	24277.0
62	22710.0
63	21143.0
64	19218.5
65	17294.0
66	15836.5
67	14379.0
68	14379.0
69	11825.5
70	9272.0
71	7872.5
72	6473.0
73	4850.5
74	3228.0
75	3228.0
76	2505.5
77	1783.0
78	1428.0
79	1073.0
80	961.5
81	850.0
82	850.0
83	678.5
84	507.0
85	474.5
86	442.0
87	383.5
88	325.0
89	325.0
90	226.0
91	127.0
92	72.0
93	17.0
94	12.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	353463.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.15863257008477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.32177480579502	48.76875976351953
2	7.187911349911104	8.217023675918215
3	2.4217073356579717	4.152644393734588
4	1.2370465553991536	2.8283155812859695
5	0.7557829423582252	2.1599759752495675
6	0.5294469325841478	1.8157477610961446
7	0.35816856152264986	1.4330697644360262
8	0.2797998261824848	1.2794380366350584
9	0.2147313292247731	1.1046374233600238
>10	1.4839741824422719	16.013646241789974
>50	0.14606378341974408	5.774874878395083
>100	0.06210194873288386	5.714104656917049
>500	9.936311797261418E-4	0.45208501211668534
>1k	4.968155898630709E-4	0.28567683554609635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1006	0.28461253370225453	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	853	0.241326532055689	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	739	0.20907421710334603	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	494	0.13976003146015284	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	379	0.10722480146436826	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.658300868832099E-4	0.0
12	0.0	0.0	0.0	8.487451303248148E-4	0.0
13	0.0	0.0	0.0	0.0011316601737664197	0.0
14	0.0	0.0	0.0	0.0011316601737664197	0.0
15	0.0	0.0	0.0	0.0019804053040912346	0.0
16	0.0	0.0	0.0	0.0025462353909744442	0.0
17	0.0	0.0	0.0	0.003394980521299259	0.0
18	0.0	0.0	0.0	0.003394980521299259	0.0
19	0.0	0.0	0.0	0.003677895564740864	0.0
20	0.0	0.0	0.0	0.003960810608182469	0.0
21	0.0	0.0	0.0	0.0042437256516240736	0.0
22	0.0	0.0	0.0	0.006507045999156913	0.0
23	0.0	0.0	0.0	0.008770366346689753	0.0
24	0.0	0.0	0.0	0.015277412345846666	0.0
25	0.0	0.0	0.0	0.020369883127795554	0.0
26	0.0	0.0	0.0	0.02744275921383568	0.0
27	0.0	0.0	0.0	0.06733378033910196	0.0
28	0.0	0.0	0.0	0.2065279817123716	0.0
29	0.0	0.0	0.0	0.4025881068174038	0.0
30	0.0	0.0	0.0	0.6546654105238738	0.0
31	0.0	0.0	0.0	1.247372426534036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTAT	30	3.5944808E-4	30.833334	1
TATACTG	25	0.0054912576	29.6	5
TGTAACA	40	0.001928557	23.125	2
GCGGTAA	65	2.6741254E-6	22.76923	34
TCTAGCG	85	5.155198E-8	21.764708	28
CGTTTAT	60	3.716896E-5	21.583334	31
TAAGCAG	80	6.932096E-7	20.8125	5
ACGCCGT	45	0.0038206463	20.555555	24
CTAAGCA	45	0.0038206463	20.555555	4
TCTAAGC	45	0.0038206463	20.555555	3
TAGGAGT	45	0.0038206463	20.555555	4
CAGTACT	45	0.0038206463	20.555555	4
TATCTAG	55	5.1334157E-4	20.181818	1
TCGTTTA	65	6.884859E-5	19.923077	30
CTATACT	65	6.884859E-5	19.923077	4
CGCGGTA	75	9.236033E-6	19.733334	33
TTAGCTC	75	9.236033E-6	19.733334	4
CTAGCGG	85	1.2403216E-6	19.588236	29
GAATTTC	105	2.24627E-8	19.38095	18
GCAGTCG	135	5.4569682E-11	19.185186	9
>>END_MODULE