FastQCFastQC Report
Fri 10 Feb 2017
ERR1631462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189036
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.22006390317188262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3690.1952009141115978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3270.1729829238875135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCG451.0718577E-732.88888531
CTAGCGG401.5907208E-632.37529
GCAATAC352.3778402E-531.71428537
ATTCCGA250.005484874429.66
TATACTG250.005484874429.65
TCTAGCG453.9850074E-628.77777528
CGCAATA451.3175062E-424.66666436
ACGGACC551.8917768E-523.5454548
CGGTCCA551.8917768E-523.54545410
AATTCAG400.001925254823.12515
GCCACGT400.001925254823.12528
ACCTCTA652.6632042E-622.7692325
CGCAAGA652.6632042E-622.769232
ATACACA855.1231837E-821.76470637
TAGCGGC603.7051243E-521.58333430
GAATTTC603.7051243E-521.58333418
GGTCCAA705.064803E-621.14285711
GGTATCA2200.021.0227281
GCCGTTC450.003814169220.5555538
AAGAATT656.863212E-519.92307716
Produced by FastQC (version 0.11.5)