##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631462.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189036
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.046139359698685	34.0	33.0	34.0	31.0	34.0
2	33.19179944560825	34.0	33.0	34.0	31.0	34.0
3	33.28033813665122	34.0	34.0	34.0	31.0	34.0
4	36.48975856450623	37.0	37.0	37.0	35.0	37.0
5	36.471010812755246	37.0	37.0	37.0	35.0	37.0
6	36.517340612370134	37.0	37.0	37.0	35.0	37.0
7	36.53608307412345	37.0	37.0	37.0	35.0	37.0
8	36.53826255316448	37.0	37.0	37.0	35.0	37.0
9	38.390396538225524	39.0	39.0	39.0	37.0	39.0
10	38.355101673755264	39.0	39.0	39.0	37.0	39.0
11	38.40298673268584	39.0	39.0	39.0	37.0	39.0
12	38.368189127996786	39.0	39.0	39.0	37.0	39.0
13	38.39384561670793	39.0	39.0	39.0	37.0	39.0
14	39.950771281660636	41.0	40.0	41.0	38.0	41.0
15	39.92954781099896	41.0	40.0	41.0	38.0	41.0
16	39.89006855836983	41.0	40.0	41.0	38.0	41.0
17	39.887883789331134	41.0	40.0	41.0	38.0	41.0
18	39.850679235701136	41.0	40.0	41.0	38.0	41.0
19	39.87214604625574	41.0	40.0	41.0	38.0	41.0
20	39.82627118644068	41.0	40.0	41.0	38.0	41.0
21	39.80298990668444	41.0	40.0	41.0	38.0	41.0
22	39.74885736050276	41.0	40.0	41.0	38.0	41.0
23	39.704125140184935	41.0	40.0	41.0	37.0	41.0
24	39.666756596627096	41.0	40.0	41.0	37.0	41.0
25	39.66773524619649	41.0	40.0	41.0	37.0	41.0
26	39.56210457267399	41.0	40.0	41.0	37.0	41.0
27	39.520546350959606	41.0	40.0	41.0	37.0	41.0
28	39.458484098267	41.0	40.0	41.0	36.0	41.0
29	39.404637211959624	41.0	39.0	41.0	36.0	41.0
30	39.336639581878586	41.0	39.0	41.0	36.0	41.0
31	39.27494233902537	41.0	39.0	41.0	35.0	41.0
32	39.19489938424427	41.0	39.0	41.0	35.0	41.0
33	39.11479823948878	41.0	39.0	41.0	35.0	41.0
34	39.05417486616306	41.0	39.0	41.0	35.0	41.0
35	38.962599716456126	41.0	39.0	41.0	35.0	41.0
36	38.884979580608984	41.0	39.0	41.0	35.0	41.0
37	38.821790558412154	40.0	38.0	41.0	35.0	41.0
38	38.74111280391037	40.0	38.0	41.0	35.0	41.0
39	38.654928161831606	40.0	38.0	41.0	35.0	41.0
40	38.54415561057153	40.0	38.0	41.0	35.0	41.0
41	38.4534427304852	40.0	38.0	41.0	35.0	41.0
42	38.329709684927735	40.0	37.0	41.0	35.0	41.0
43	37.53116337628811	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	3.0
20	14.0
21	20.0
22	38.0
23	72.0
24	105.0
25	182.0
26	247.0
27	364.0
28	538.0
29	774.0
30	997.0
31	1364.0
32	1653.0
33	2293.0
34	3247.0
35	5190.0
36	8222.0
37	15644.0
38	34880.0
39	113185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.862015700713094	17.015806513045135	11.847478787109333	27.27469899913244
2	20.252756088787322	19.027592627859242	32.262108804672124	28.457542478681308
3	21.168983685647177	20.33845405108022	27.77777777777778	30.714784485494828
4	15.789585052582577	14.919909435239848	31.410948179182803	37.87955733299477
5	17.887069129689586	33.276201358471404	30.951776381218394	17.884953130620623
6	36.68295985949766	31.527857127742863	15.205569309549505	16.58361370320997
7	31.887047969698894	28.229543579000826	19.215387545229483	20.6680209060708
8	29.419264055523815	30.604223534141646	18.517107852472545	21.459404557861994
9	27.917433716329164	13.773566939630546	18.322435938128187	39.9865634059121
10	19.816860280581476	24.23824033517425	29.73031591866099	26.214583465583274
11	38.584185022958586	20.068135170020525	19.17941556105715	22.16826424596373
12	24.01394443386445	23.49711166127087	26.39973338411731	26.08921052074737
13	32.6350536405764	17.487145305656064	22.67874902135043	27.199052032417104
14	24.54664719947523	18.825514716773526	22.530629086523202	34.09720899722804
15	27.8031697666053	25.04602297974989	20.127912143718657	27.022895109926154
16	27.44027592627859	23.621426606572292	21.924924353033283	27.01337311411583
17	26.18495947861783	24.163651367993396	23.059628853763304	26.591760299625467
18	26.59334729892719	22.488309105143994	24.148310374743435	26.770033221185386
19	26.778497217461222	23.64999259400326	23.6505215937705	25.92098859476502
20	27.184769038701624	22.67187202437631	23.55635963520176	26.58699930172031
21	28.15230961298377	22.913095918237797	22.942190905436004	25.99240356334243
22	28.47605747053471	22.461859116781987	22.46662011468715	26.595463297996147
23	26.932436149728094	22.670814024841828	23.14585581582344	27.250894009606636
24	26.782729215599144	23.249539770202503	23.107767832582155	26.859963181616198
25	26.75416322816818	22.344421168454687	23.35004972597812	27.551365877399014
26	26.555259315685902	23.04481686028058	23.424109693391735	26.975814130641783
27	26.936668147866015	22.609979051609216	23.20457478998709	27.248778010537677
28	26.244207452548725	22.8115279629277	23.738864554899596	27.205400029623988
29	25.83052963456696	23.19981379208193	24.050445417804017	26.91921115554709
30	25.933684589178778	23.54207664148628	23.9737404515542	26.55049831778074
31	26.699147252375212	23.19769779301297	23.12839882350452	26.974756131107302
32	25.62686472417952	22.959647897754927	23.959457457838717	27.454029920226837
33	25.454410800059247	22.56660107069553	24.5937281787596	27.385259950485626
34	26.475909350599885	22.221164222687744	24.2731543198121	27.029772106900275
35	25.997164561247594	22.26930320150659	24.85717006284517	26.876362174400644
36	25.165047927378914	22.483019107471595	25.038616983008527	27.313315982140967
37	25.45282380075753	21.38058359254322	25.669713705325968	27.496878901373282
38	24.513849213906347	21.06794473010432	26.8678981781248	27.550307877864533
39	24.70111513150934	20.804502846018746	27.12975306290865	27.36462895956326
40	24.360968281173957	20.85475782390656	27.659281829915994	27.12499206500349
41	23.24054677415942	21.122431706130048	28.61359741001714	27.02342410969339
42	23.206161789288814	21.345669607905375	28.532131445862163	26.916037156943652
43	22.346537167523646	20.77064686091538	28.907721280602637	27.975094690958336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	20.0
2	22.0
3	46.5
4	71.0
5	71.0
6	91.0
7	111.0
8	97.5
9	84.0
10	140.0
11	196.0
12	196.0
13	329.5
14	463.0
15	681.0
16	899.0
17	840.0
18	781.0
19	781.0
20	909.5
21	1038.0
22	913.5
23	789.0
24	885.5
25	982.0
26	982.0
27	1180.0
28	1378.0
29	1637.5
30	1897.0
31	2273.0
32	2649.0
33	2649.0
34	3219.5
35	3790.0
36	4531.5
37	5273.0
38	6151.0
39	7029.0
40	7029.0
41	7621.5
42	8214.0
43	8908.0
44	9602.0
45	10296.0
46	10990.0
47	10990.0
48	12020.0
49	13050.0
50	13513.5
51	13977.0
52	14321.5
53	14666.0
54	14666.0
55	14479.5
56	14293.0
57	14227.0
58	14161.0
59	13796.5
60	13432.0
61	13432.0
62	12622.0
63	11812.0
64	11018.0
65	10224.0
66	9263.5
67	8303.0
68	8303.0
69	7207.5
70	6112.0
71	5373.0
72	4634.0
73	3718.0
74	2802.0
75	2802.0
76	2327.5
77	1853.0
78	1561.0
79	1269.0
80	1068.5
81	868.0
82	868.0
83	716.0
84	564.0
85	461.0
86	358.0
87	297.5
88	237.0
89	237.0
90	174.5
91	112.0
92	66.0
93	20.0
94	13.5
95	7.0
96	7.0
97	5.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	189036.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.13446069440447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04067226862045	65.11941546232772
2	6.645435910993623	9.720207428594941
3	1.699527825376033	3.7288215283203057
4	0.7172189137426204	2.098136738255725
5	0.4274808424101447	1.5631790433427821
6	0.28487760637287646	1.2500622063595896
7	0.19923635698145523	1.0199730462989605
8	0.13275787229221744	0.7767340314422355
9	0.09113081468091486	0.5998322685897383
>10	0.6982726406527007	10.08821779083856
>50	0.05150352015003798	2.4454459627005685
>100	0.011885427726931842	1.589974492928835
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.22006390317188262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	369	0.1952009141115978	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.1729829238875135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.289997672401024E-4	0.0	0.0	0.0
6	0.0	5.289997672401024E-4	0.0	0.0	0.0
7	0.0	5.289997672401024E-4	0.0	0.0	0.0
8	0.0	5.289997672401024E-4	0.0	0.0	0.0
9	0.0	5.289997672401024E-4	0.0	0.0	0.0
10	0.0	5.289997672401024E-4	0.0	0.0	5.289997672401024E-4
11	0.0	0.001057999534480205	0.0	0.0	5.289997672401024E-4
12	0.0	0.001057999534480205	0.0	0.0	5.289997672401024E-4
13	0.0	0.001057999534480205	0.0	0.0	0.001057999534480205
14	0.0	0.001057999534480205	0.0	0.0	0.001057999534480205
15	0.0	0.001057999534480205	0.0	0.0	0.001057999534480205
16	0.0	0.001057999534480205	0.0	5.289997672401024E-4	0.001057999534480205
17	0.0	0.001057999534480205	0.0	5.289997672401024E-4	0.001057999534480205
18	0.0	0.001057999534480205	0.0	5.289997672401024E-4	0.001057999534480205
19	0.0	0.001057999534480205	0.0	5.289997672401024E-4	0.001057999534480205
20	0.0	0.001057999534480205	0.0	0.001057999534480205	0.001057999534480205
21	0.0	0.001057999534480205	0.0	0.00211599906896041	0.001057999534480205
22	0.0	0.001057999534480205	0.0	0.0031739986034406147	0.001057999534480205
23	0.0	0.001057999534480205	0.0	0.004760997905160921	0.001057999534480205
24	0.0	0.001057999534480205	0.0	0.010050995577561946	0.001057999534480205
25	0.0	0.001057999534480205	0.0	0.014811993482722868	0.001057999534480205
26	0.0	0.001057999534480205	0.0	0.02380498952580461	0.0015869993017203073
27	0.0	0.001057999534480205	0.0	0.08358196322393618	0.0015869993017203073
28	0.0	0.001057999534480205	0.0	0.27349287966313296	0.0015869993017203073
29	0.0	0.001057999534480205	0.0	0.5083687763177385	0.0015869993017203073
30	0.0	0.001057999534480205	0.0	0.7940286506273937	0.0015869993017203073
31	0.0	0.001057999534480205	0.0	1.436763367824118	0.0015869993017203073
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGGCG	45	1.0718577E-7	32.888885	31
CTAGCGG	40	1.5907208E-6	32.375	29
GCAATAC	35	2.3778402E-5	31.714285	37
ATTCCGA	25	0.0054848744	29.6	6
TATACTG	25	0.0054848744	29.6	5
TCTAGCG	45	3.9850074E-6	28.777775	28
CGCAATA	45	1.3175062E-4	24.666664	36
ACGGACC	55	1.8917768E-5	23.545454	8
CGGTCCA	55	1.8917768E-5	23.545454	10
AATTCAG	40	0.0019252548	23.125	15
GCCACGT	40	0.0019252548	23.125	28
ACCTCTA	65	2.6632042E-6	22.76923	25
CGCAAGA	65	2.6632042E-6	22.76923	2
ATACACA	85	5.1231837E-8	21.764706	37
TAGCGGC	60	3.7051243E-5	21.583334	30
GAATTTC	60	3.7051243E-5	21.583334	18
GGTCCAA	70	5.064803E-6	21.142857	11
GGTATCA	220	0.0	21.022728	1
GCCGTTC	45	0.0038141692	20.555553	8
AAGAATT	65	6.863212E-5	19.923077	16
>>END_MODULE