FastQCFastQC Report
Fri 10 Feb 2017
ERR1631461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332967
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.15286800193412559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4190.1258382962876201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3740.11232344346436733No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3720.11172278333888942No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3370.10121123114302619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTA250.00549080729.633
ATTGGGC451.320456E-424.6666663
AATTTAA400.001928322623.12500223
GGTATCA4250.023.070591
TCGCACG652.6733524E-622.7692322
GGCCGAT652.6733524E-622.7692316
ATCGCAC652.6733524E-622.7692321
AATAACG502.69652E-422.210
GATCGCA705.0839844E-621.14285720
CCGATCG705.0839844E-621.14285718
TAGATAA806.929604E-720.81254
AGTCGGT3400.020.6764711
TAATATA450.003820187620.5555554
CAGTCGG3450.020.37681210
CCTACAC555.1325554E-420.1818183
GCAGTCG3500.020.0857149
CGCCTTA2600.019.92307725
GATAACC759.233392E-619.7333346
CGATCGC759.233392E-619.73333419
CGAGCGA851.2398796E-619.58823410
Produced by FastQC (version 0.11.5)