FastQCFastQC Report
Fri 10 Feb 2017
ERR1631460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838239
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.18288340198917014No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12500.14912214774068017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12450.14852565914971744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11700.13957833028527664No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9800.11691176382869325No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9590.11440651174664983No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9480.11309423684653183No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG9050.10796443496425244No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8710.10390831254570593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG303.5986552E-430.8333326
ACCGGGC400.001930753423.1258
ACTATTC1850.023.015
TAGAACT1600.021.968754
TCTAGCG1550.021.48387128
CTAGCGG1550.021.48387129
CACTATT1900.021.42105314
CTAAACA705.099202E-621.1428594
ATACACA3450.020.91304237
GGTATCA9100.020.7362631
CAATTCT450.003824957420.5555554
TATACTT450.003824957420.5555555
GGCACTA2000.020.3512
TTAGCGG555.141498E-420.18181815
GCACTAT2100.019.3809513
TTACACT2500.019.244
TATACAC6000.018.80833437
CGTGAAT1306.9485395E-1018.510
TTAGAAC701.21869736E-418.53
ATTACAC500.00703333218.53
Produced by FastQC (version 0.11.5)