##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838239
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.10889495716616	34.0	33.0	34.0	31.0	34.0
2	33.249242757733775	34.0	33.0	34.0	31.0	34.0
3	33.33488420366984	34.0	34.0	34.0	31.0	34.0
4	36.5311802481154	37.0	37.0	37.0	35.0	37.0
5	36.530959547336735	37.0	37.0	37.0	35.0	37.0
6	36.5862659694908	37.0	37.0	37.0	35.0	37.0
7	36.594886422607395	37.0	37.0	37.0	35.0	37.0
8	36.5867479322723	37.0	37.0	37.0	35.0	37.0
9	38.44269355160044	39.0	39.0	39.0	37.0	39.0
10	38.411259795833885	39.0	39.0	39.0	37.0	39.0
11	38.466315692779744	39.0	39.0	39.0	37.0	39.0
12	38.43097255078802	39.0	39.0	39.0	37.0	39.0
13	38.45213596599538	39.0	39.0	39.0	37.0	39.0
14	40.02242916399738	41.0	40.0	41.0	38.0	41.0
15	40.019081670024896	41.0	40.0	41.0	38.0	41.0
16	40.01009020100472	41.0	40.0	41.0	38.0	41.0
17	39.99941424820368	41.0	40.0	41.0	38.0	41.0
18	39.979091881909575	41.0	40.0	41.0	38.0	41.0
19	39.9827531288809	41.0	40.0	41.0	38.0	41.0
20	39.964090193846864	41.0	40.0	41.0	38.0	41.0
21	39.92808494951917	41.0	40.0	41.0	38.0	41.0
22	39.89020553803867	41.0	40.0	41.0	38.0	41.0
23	39.834432661806474	41.0	40.0	41.0	38.0	41.0
24	39.80754892101179	41.0	40.0	41.0	38.0	41.0
25	39.7871931513566	41.0	40.0	41.0	38.0	41.0
26	39.71713795230239	41.0	40.0	41.0	37.0	41.0
27	39.6650012705207	41.0	40.0	41.0	37.0	41.0
28	39.592582783669094	41.0	40.0	41.0	37.0	41.0
29	39.55560287698377	41.0	40.0	41.0	37.0	41.0
30	39.49022653443708	41.0	40.0	41.0	37.0	41.0
31	39.44677830547135	41.0	39.0	41.0	36.0	41.0
32	39.37051843209395	41.0	39.0	41.0	36.0	41.0
33	39.30398967358951	41.0	39.0	41.0	35.0	41.0
34	39.24105535533422	41.0	39.0	41.0	35.0	41.0
35	39.15947599670261	41.0	39.0	41.0	35.0	41.0
36	39.09232331113203	41.0	39.0	41.0	35.0	41.0
37	39.03549584307101	41.0	39.0	41.0	35.0	41.0
38	38.95837106123671	41.0	39.0	41.0	35.0	41.0
39	38.89240538796214	40.0	39.0	41.0	35.0	41.0
40	38.80710513350011	40.0	38.0	41.0	35.0	41.0
41	38.728898321361804	40.0	38.0	41.0	35.0	41.0
42	38.59630845140825	40.0	38.0	41.0	35.0	41.0
43	37.78151815890217	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	6.0
17	4.0
18	12.0
19	18.0
20	33.0
21	77.0
22	143.0
23	243.0
24	410.0
25	608.0
26	914.0
27	1335.0
28	1818.0
29	2607.0
30	3564.0
31	4638.0
32	6062.0
33	8221.0
34	12220.0
35	18679.0
36	32021.0
37	64573.0
38	158039.0
39	521993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18715903220919	16.760136428870524	11.98071194492263	27.071992593997656
2	20.08174279650553	18.88781123283455	33.69289665596566	27.33754931469426
3	21.13203990747269	19.763933675240594	27.698902103099478	31.405124314187244
4	15.502619181402919	14.337438367816338	31.285230107403734	38.87471234337701
5	17.19127838241838	33.51872198740455	31.395341901295453	17.89465772888162
6	37.14417964327596	31.59719364047724	14.937983081197606	16.320643635049194
7	32.099437034067854	28.198640244608043	18.846057031467158	20.85586568985695
8	28.966440358895255	31.054508320419355	18.748948688858427	21.23010263182696
9	28.241348827720973	13.83018447006164	18.337132965657766	39.59133373655962
10	19.116743554046042	24.23127532839679	29.499104670624966	27.1528764469322
11	38.76328827458517	20.153798618293827	19.40782998643585	21.67508312068515
12	25.005040328593637	23.56857650383721	25.900250405910484	25.526132761658666
13	32.17113496270157	17.219194048475433	23.467292741091743	27.14237824773126
14	24.861644471326198	18.64122284933056	22.133305656262714	34.36382702308053
15	27.090364442599306	25.46254707786204	19.702614648089625	27.744473831449024
16	27.578292110006814	23.709825002177183	21.401891346024225	27.309991541791778
17	26.80178326229154	24.233780580478836	22.427016638452756	26.53741951877687
18	27.525920411720286	21.91367855707024	23.80812632196784	26.752274709241636
19	27.23077785691193	22.998452708595043	23.28476723225715	26.486002202235877
20	27.161107989487487	22.14535472580016	23.19708340938563	27.496453875326726
21	28.60759282257208	22.402560606223286	23.26329364298249	25.72655292822214
22	28.860623282858466	22.001720273096335	22.320960966979584	26.81669547706561
23	26.825404210493666	22.186035247703817	23.166781788964723	27.821778752837794
24	26.762057122133427	22.966958110992213	22.94822836923598	27.322756397638383
25	27.143213331758602	22.389318559503913	22.867583111737822	27.599884996999663
26	26.69715916343668	23.328430197115622	23.090431249321494	26.883979390126207
27	27.938213325793715	22.04955865809155	23.092817203685346	26.91941081242939
28	26.34880982631445	22.725141636215923	23.56798001524625	27.358068522223377
29	26.152087889014947	22.770355471410898	23.70397941398575	27.37357722558841
30	25.713788072375536	22.806621977741433	24.55003883140727	26.929551118475757
31	26.833397157612566	22.395402743131733	23.024698206597403	27.746501892658298
32	26.068340890843782	21.969867782338927	24.042784933652577	27.919006393164718
33	26.210185877774716	21.964976575893033	23.71006359761357	28.114773948718685
34	26.92919322532118	21.73783372045443	24.143114314652504	27.189858739571886
35	26.196466640182575	21.547315264500934	25.003250862820746	27.252967232495745
36	25.029854253977685	21.537174958454568	25.648055029651445	27.784915757916302
37	26.06702861594366	20.636835079255437	25.98340091549069	27.312735389310212
38	24.94097745392424	19.895757653843354	27.234237490739517	27.92902740149289
39	25.569199237926178	20.14819162553878	27.128420414702724	27.154188721832316
40	24.43038322006015	21.194313316369197	26.8040499189372	27.57125354463345
41	23.78701062584776	21.249070969019577	28.325453719046717	26.63846468608595
42	24.268973407345637	21.197415057042203	27.674684666306387	26.858926869305773
43	22.872116425029137	20.940924962928236	27.648319870585837	28.53863874145679
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	90.5
2	83.0
3	192.5
4	302.0
5	302.0
6	350.5
7	399.0
8	383.0
9	367.0
10	542.0
11	717.0
12	717.0
13	1111.0
14	1505.0
15	2237.0
16	2969.0
17	2769.0
18	2569.0
19	2569.0
20	3026.0
21	3483.0
22	3097.5
23	2712.0
24	3058.0
25	3404.0
26	3404.0
27	3913.0
28	4422.0
29	5403.5
30	6385.0
31	7640.5
32	8896.0
33	8896.0
34	11427.5
35	13959.0
36	17234.0
37	20509.0
38	24257.0
39	28005.0
40	28005.0
41	31038.5
42	34072.0
43	36109.5
44	38147.0
45	43505.5
46	48864.0
47	48864.0
48	53399.5
49	57935.0
50	60130.5
51	62326.0
52	66461.0
53	70596.0
54	70596.0
55	72185.0
56	73774.0
57	73174.5
58	72575.0
59	70034.0
60	67493.0
61	67493.0
62	64408.0
63	61323.0
64	55938.5
65	50554.0
66	44631.5
67	38709.0
68	38709.0
69	31743.0
70	24777.0
71	20767.5
72	16758.0
73	12174.0
74	7590.0
75	7590.0
76	5697.0
77	3804.0
78	3025.5
79	2247.0
80	2163.0
81	2079.0
82	2079.0
83	1705.0
84	1331.0
85	1273.5
86	1216.0
87	1032.5
88	849.0
89	849.0
90	597.0
91	345.0
92	205.0
93	65.0
94	41.0
95	17.0
96	17.0
97	12.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	838239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05538254898129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.88503445688562	34.439321797619165
2	6.992223622406304	5.741368313718291
3	2.4887549984902213	3.065303656011162
4	1.3753196910802779	2.2585710417779032
5	0.8534482194817234	1.7519321568284552
6	0.589274782099675	1.451574096334185
7	0.459612725931579	1.3208703401250748
8	0.3455653777330246	1.1349855022810038
9	0.27548936744921143	1.0179289231923825
>10	2.19361060660037	18.595615703290296
>50	0.3216143055694797	9.255379333824013
>100	0.2072106244741397	16.242112683511877
>500	0.011673837998541466	3.102220038130176
>1k	0.0011673837998541468	0.6228164133560177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1533	0.18288340198917014	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1250	0.14912214774068017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1245	0.14852565914971744	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.13957833028527664	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	980	0.11691176382869325	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	959	0.11440651174664983	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	948	0.11309423684653183	No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	905	0.10796443496425244	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	871	0.10390831254570593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1929771819254413E-4	0.0	0.0	0.0
6	0.0	1.1929771819254413E-4	0.0	0.0	0.0
7	0.0	1.1929771819254413E-4	0.0	0.0	0.0
8	0.0	1.1929771819254413E-4	0.0	0.0	0.0
9	0.0	1.1929771819254413E-4	0.0	1.1929771819254413E-4	0.0
10	0.0	1.1929771819254413E-4	0.0	4.771908727701765E-4	0.0
11	0.0	1.1929771819254413E-4	0.0	7.157863091552648E-4	0.0
12	0.0	1.1929771819254413E-4	0.0	7.157863091552648E-4	0.0
13	0.0	1.1929771819254413E-4	0.0	7.157863091552648E-4	0.0
14	0.0	1.1929771819254413E-4	0.0	9.54381745540353E-4	0.0
15	0.0	1.1929771819254413E-4	0.0	0.0011929771819254413	0.0
16	0.0	2.3859543638508825E-4	0.0	0.001789465772888162	0.0
17	0.0	2.3859543638508825E-4	0.0	0.0023859543638508826	0.0
18	0.0	3.578931545776324E-4	0.0	0.0023859543638508826	0.0
19	0.0	3.578931545776324E-4	0.0	0.0028631452366210592	0.0
20	0.0	3.578931545776324E-4	0.0	0.0031017406730061473	0.0
21	0.0	3.578931545776324E-4	0.0	0.004294717854931589	0.0
22	0.0	3.578931545776324E-4	0.0	0.005368397318664486	0.0
23	0.0	4.771908727701765E-4	0.0	0.007038565373360103	0.0
24	0.0	4.771908727701765E-4	0.0	0.012168367255639502	0.0
25	0.0	4.771908727701765E-4	0.0	0.015747298801415826	0.0
26	0.0	4.771908727701765E-4	0.0	0.024336734511279004	0.0
27	0.0	4.771908727701765E-4	0.0	0.0821961278346629	0.0
28	0.0	4.771908727701765E-4	0.0	0.3273529387203411	0.0
29	0.0	4.771908727701765E-4	0.0	0.662340931405005	0.0
30	0.0	4.771908727701765E-4	0.0	1.1103038632180082	0.0
31	0.0	4.771908727701765E-4	0.0	2.0404681719652746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGG	30	3.5986552E-4	30.833332	6
ACCGGGC	40	0.0019307534	23.125	8
ACTATTC	185	0.0	23.0	15
TAGAACT	160	0.0	21.96875	4
TCTAGCG	155	0.0	21.483871	28
CTAGCGG	155	0.0	21.483871	29
CACTATT	190	0.0	21.421053	14
CTAAACA	70	5.099202E-6	21.142859	4
ATACACA	345	0.0	20.913042	37
GGTATCA	910	0.0	20.736263	1
CAATTCT	45	0.0038249574	20.555555	4
TATACTT	45	0.0038249574	20.555555	5
GGCACTA	200	0.0	20.35	12
TTAGCGG	55	5.141498E-4	20.181818	15
GCACTAT	210	0.0	19.38095	13
TTACACT	250	0.0	19.24	4
TATACAC	600	0.0	18.808334	37
CGTGAAT	130	6.9485395E-10	18.5	10
TTAGAAC	70	1.21869736E-4	18.5	3
ATTACAC	50	0.007033332	18.5	3
>>END_MODULE