FastQCFastQC Report
Fri 10 Feb 2017
ERR1631459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121798
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.20984170055571502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22880.2039582883906015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18800.16758810409717256No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18720.16687496322867396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15980.14244988848259668No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC13270.11829224156220637No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13140.11713338765089615No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12760.11374596852552776No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC11730.10456427984360821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.022.4950161
ACGGAGT1900.021.42105326
AACGGAG2050.019.8536625
GTTACAC759.265446E-619.7333343
GTCTAGA2550.018.8627451
CTAGAAC2950.018.8135593
TATTCCG500.007034779518.527
TATCGCC500.007034779518.533
ATACACA4500.018.537
ACTGATC2350.018.1063848
TACACAG1451.546141E-1017.8620685
ACAACGG2300.017.69565223
CCGCTTA953.6062302E-617.52631625
CGGAGTC2500.017.0227
CAACGGA2400.016.95833424
ATTACAA2400.016.95833420
GTGTTAC803.3824757E-416.18751
TAGCTCC803.3824757E-416.18754
CTATATA700.00259244115.8571432
CTATTTG1059.342621E-615.8571423
Produced by FastQC (version 0.11.5)