##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1121798
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08020249634961	34.0	33.0	34.0	31.0	34.0
2	33.22233414571964	34.0	33.0	34.0	31.0	34.0
3	33.304274922936216	34.0	34.0	34.0	31.0	34.0
4	36.518180635016286	37.0	37.0	37.0	35.0	37.0
5	36.49826528483738	37.0	37.0	37.0	35.0	37.0
6	36.54264938964056	37.0	37.0	37.0	35.0	37.0
7	36.5622153007939	37.0	37.0	37.0	35.0	37.0
8	36.56290437315809	37.0	37.0	37.0	35.0	37.0
9	38.41723197937596	39.0	39.0	39.0	37.0	39.0
10	38.391121217901976	39.0	39.0	39.0	37.0	39.0
11	38.43200469246691	39.0	39.0	39.0	37.0	39.0
12	38.40536353247198	39.0	39.0	39.0	37.0	39.0
13	38.42434110240881	39.0	39.0	39.0	37.0	39.0
14	39.99806471396811	41.0	40.0	41.0	38.0	41.0
15	39.976367403044044	41.0	40.0	41.0	38.0	41.0
16	39.93993838462896	41.0	40.0	41.0	38.0	41.0
17	39.93984032775954	41.0	40.0	41.0	38.0	41.0
18	39.91344965849466	41.0	40.0	41.0	38.0	41.0
19	39.920580175753564	41.0	40.0	41.0	38.0	41.0
20	39.88156156455975	41.0	40.0	41.0	38.0	41.0
21	39.85844153760303	41.0	40.0	41.0	38.0	41.0
22	39.79337278190904	41.0	40.0	41.0	38.0	41.0
23	39.74421598184343	41.0	40.0	41.0	38.0	41.0
24	39.71281460655127	41.0	40.0	41.0	38.0	41.0
25	39.72315069201407	41.0	40.0	41.0	37.0	41.0
26	39.63494497226773	41.0	40.0	41.0	37.0	41.0
27	39.59207005182751	41.0	40.0	41.0	37.0	41.0
28	39.53306655922011	41.0	40.0	41.0	37.0	41.0
29	39.49927794487065	41.0	40.0	41.0	37.0	41.0
30	39.449299249954095	41.0	40.0	41.0	36.0	41.0
31	39.409882171300005	41.0	39.0	41.0	36.0	41.0
32	39.343839086894434	41.0	39.0	41.0	36.0	41.0
33	39.276672805620976	41.0	39.0	41.0	35.0	41.0
34	39.22720222357323	41.0	39.0	41.0	35.0	41.0
35	39.149042875811865	41.0	39.0	41.0	35.0	41.0
36	39.092647695931	41.0	39.0	41.0	35.0	41.0
37	39.03278397715096	41.0	39.0	41.0	35.0	41.0
38	38.96418517415791	41.0	39.0	41.0	35.0	41.0
39	38.875149536725864	41.0	39.0	41.0	35.0	41.0
40	38.78464304625253	40.0	38.0	41.0	35.0	41.0
41	38.69552539762061	40.0	38.0	41.0	35.0	41.0
42	38.572805442691106	40.0	38.0	41.0	35.0	41.0
43	37.80826940322589	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	1.0
14	0.0
15	2.0
16	2.0
17	6.0
18	12.0
19	26.0
20	66.0
21	107.0
22	189.0
23	304.0
24	537.0
25	919.0
26	1363.0
27	2010.0
28	2946.0
29	4201.0
30	5573.0
31	7487.0
32	9603.0
33	12110.0
34	17812.0
35	26321.0
36	43368.0
37	83654.0
38	197596.0
39	705580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64725200080585	17.66717359096736	12.126158185341746	26.559416222885048
2	19.846977798141914	19.61003674458325	33.32025908407753	27.222726373197315
3	20.666911511698185	20.92087880349225	28.178691707419695	30.233517977389866
4	15.12081497738452	15.108335012185794	32.498631660958566	37.27221834947112
5	16.69881743415481	34.45629248759581	31.6834225056561	17.161467572593285
6	35.76303398651094	32.65712721898238	15.638287820088822	15.941550974417854
7	31.12280464040763	29.07368349738545	19.76897801564988	20.034533846557046
8	28.576802597259043	30.98953644060695	19.410268158795077	21.023392803338925
9	27.635456650840883	14.122596046703595	19.07509195060073	39.16685535185479
10	19.413744720529007	24.585798869315152	30.229149989570313	25.77130642058553
11	37.677460647995446	20.857587551412998	19.860616617251946	21.604335183339604
12	23.750086914043347	24.508957940734426	26.717198640040362	25.02375650518186
13	31.071191070050048	18.11137120943343	24.365438340949083	26.451999379567443
14	24.1330435604271	19.61859443500523	23.259356853907747	32.98900515065992
15	27.175569933267845	25.87426613347501	20.751775275049518	26.198388658207627
16	26.882379893706354	24.53249158939488	22.534984016730284	26.050144500168482
17	25.768364714502965	25.129836209371025	23.2691625408496	25.832636535276404
18	26.09061524445578	22.778075910279746	25.01493138693419	26.116377458330287
19	26.006464621972942	23.951905779828454	24.275493448909696	25.76613614928891
20	26.070379872312127	23.089272756770825	24.433632436499263	26.406714934417785
21	27.539271776202135	23.520722982212483	24.138124689115152	24.80188055247023
22	27.660862294281145	22.861959104936897	23.481678519662186	25.995500081119772
23	26.099083792269195	22.97704221259086	24.203287935974213	26.72058605916573
24	25.457881008880385	23.975439428488908	24.121989876965372	26.444689685665335
25	26.315343760641397	23.105407568920608	24.06458203705123	26.514666633386756
26	26.286907268510017	23.904838482507547	24.01314675191077	25.79510749707167
27	26.78566016341623	22.83869288410213	24.148197803882695	26.227449148598946
28	25.42882051848907	23.28913048516756	24.743848714296156	26.538200282047214
29	25.111561974615746	23.488453357912924	24.872303213234467	26.527681454236856
30	24.43229529737083	24.165045756900973	25.607729733873658	25.79492921185454
31	26.133403696565694	23.537570935230764	23.905908193810294	26.42311717439325
32	25.480077518412408	23.192678182703126	24.52785617374964	26.799388125134826
33	25.013505105197194	22.861513391894086	25.089187179866606	27.03579432304212
34	26.22388344425645	22.552099397574253	24.725217909106632	26.498799249062664
35	25.48248436884359	22.613162084439445	25.748396770185007	26.15595677653196
36	24.5492503998046	22.96830623695175	25.707302027637773	26.775141335605873
37	25.529105953121682	21.96554103323415	26.088832392284527	26.41652062135964
38	24.427927309551272	21.46322243398544	27.02170979088927	27.087140465574016
39	25.045685586888194	21.50030575914737	27.128770063772624	26.32523859019182
40	24.079914565723954	22.107010353022556	27.337274625199903	26.475800456053584
41	23.506638450059636	22.08989497217859	28.201244787385964	26.202221790375805
42	23.985690828473576	22.15220565556366	28.049880638047135	25.81222287791563
43	22.538282293247093	21.831737977782094	27.94879291993746	27.681186809033353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	229.0
2	204.0
3	410.0
4	616.0
5	616.0
6	721.0
7	826.0
8	788.0
9	750.0
10	1113.5
11	1477.0
12	1477.0
13	2355.0
14	3233.0
15	4238.0
16	5243.0
17	4892.5
18	4542.0
19	4542.0
20	5302.5
21	6063.0
22	5189.5
23	4316.0
24	4873.0
25	5430.0
26	5430.0
27	6530.0
28	7630.0
29	9326.5
30	11023.0
31	13089.0
32	15155.0
33	15155.0
34	19479.5
35	23804.0
36	28951.0
37	34098.0
38	39292.0
39	44486.0
40	44486.0
41	49219.5
42	53953.0
43	58533.0
44	63113.0
45	69543.5
46	75974.0
47	75974.0
48	82058.0
49	88142.0
50	90014.5
51	91887.0
52	93601.0
53	95315.0
54	95315.0
55	91322.0
56	87329.0
57	83458.0
58	79587.0
59	76940.5
60	74294.0
61	74294.0
62	70203.0
63	66112.0
64	61744.0
65	57376.0
66	51112.5
67	44849.0
68	44849.0
69	36614.0
70	28379.0
71	24059.0
72	19739.0
73	14699.5
74	9660.0
75	9660.0
76	7452.0
77	5244.0
78	4356.0
79	3468.0
80	3229.5
81	2991.0
82	2991.0
83	2406.5
84	1822.0
85	1670.5
86	1519.0
87	1350.0
88	1181.0
89	1181.0
90	876.5
91	572.0
92	333.0
93	94.0
94	65.5
95	37.0
96	37.0
97	24.0
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1121798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.73665254537561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5261413075111	42.040473212129996
2	7.516202800446364	7.476615342927599
3	2.610579032455049	3.895243868383788
4	1.336005147075096	2.6579369519562994
5	0.814444098434257	2.025386157072816
6	0.5475064529747631	1.633868293077409
7	0.37164207954372247	1.2938963087054878
8	0.2913848465073403	1.1594005494178552
9	0.23499697213397222	1.0519166477018422
>10	1.4409824012007546	13.900953306542347
>50	0.17245540378914917	5.992313998234219
>100	0.12529161637293243	11.849997252128368
>500	0.010575400750356323	3.5877528226964968
>1k	0.0017924408051451395	1.4342452890254755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2354	0.20984170055571502	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2288	0.2039582883906015	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1880	0.16758810409717256	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1872	0.16687496322867396	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1598	0.14244988848259668	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1327	0.11829224156220637	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1314	0.11713338765089615	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1276	0.11374596852552776	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1173	0.10456427984360821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.914260856232584E-5	0.0
10	0.0	0.0	0.0	8.914260856232584E-5	0.0
11	0.0	8.914260856232584E-5	0.0	2.674278256869775E-4	0.0
12	0.0	8.914260856232584E-5	0.0	6.239982599362809E-4	0.0
13	0.0	8.914260856232584E-5	0.0	7.131408684986067E-4	0.0
14	0.0	8.914260856232584E-5	0.0	9.805686941855843E-4	0.0
15	0.0	8.914260856232584E-5	0.0	0.001693709562684191	0.0
16	0.0	8.914260856232584E-5	0.0	0.002585135648307449	0.0
17	0.0	8.914260856232584E-5	0.0	0.003476561733930708	0.0
18	0.0	8.914260856232584E-5	0.0	0.0036548469510553593	0.0
19	0.0	8.914260856232584E-5	0.0	0.004546273036678618	0.0
20	0.0	8.914260856232584E-5	0.0	0.005259413905177224	0.0
21	0.0	8.914260856232584E-5	0.0	0.006329125207925134	0.0
22	0.0	8.914260856232584E-5	0.0	0.008646833030545606	0.0
23	0.0	8.914260856232584E-5	0.0	0.012212537373038639	0.0
24	0.0	8.914260856232584E-5	0.0	0.01818509214671447	0.0
25	0.0	8.914260856232584E-5	0.0	0.022196509532019133	8.914260856232584E-5
26	0.0	8.914260856232584E-5	0.0	0.034943902556431726	8.914260856232584E-5
27	0.0	8.914260856232584E-5	0.0	0.09137117377638398	8.914260856232584E-5
28	0.0	8.914260856232584E-5	0.0	0.2693889630753487	8.914260856232584E-5
29	0.0	8.914260856232584E-5	0.0	0.49768318360346514	8.914260856232584E-5
30	0.0	8.914260856232584E-5	0.0	0.8246582718100763	8.914260856232584E-5
31	0.0	8.914260856232584E-5	0.0	1.469961615192753	8.914260856232584E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1505	0.0	22.495016	1
ACGGAGT	190	0.0	21.421053	26
AACGGAG	205	0.0	19.85366	25
GTTACAC	75	9.265446E-6	19.733334	3
GTCTAGA	255	0.0	18.862745	1
CTAGAAC	295	0.0	18.813559	3
TATTCCG	50	0.0070347795	18.5	27
TATCGCC	50	0.0070347795	18.5	33
ATACACA	450	0.0	18.5	37
ACTGATC	235	0.0	18.106384	8
TACACAG	145	1.546141E-10	17.862068	5
ACAACGG	230	0.0	17.695652	23
CCGCTTA	95	3.6062302E-6	17.526316	25
CGGAGTC	250	0.0	17.02	27
CAACGGA	240	0.0	16.958334	24
ATTACAA	240	0.0	16.958334	20
GTGTTAC	80	3.3824757E-4	16.1875	1
TAGCTCC	80	3.3824757E-4	16.1875	4
CTATATA	70	0.002592441	15.857143	2
CTATTTG	105	9.342621E-6	15.857142	3
>>END_MODULE