FastQCFastQC Report
Fri 10 Feb 2017
ERR1631458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247588
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.2199246481116646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39900.17752363867399185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36970.16448744164855836No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31200.13881547685785828No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24590.1094061723056005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.024.116071
CTAGACA6650.021.1428574
AACCGAT450.003826929720.5555557
ACTGATC5750.020.2695668
TCTAGAC6700.019.8805963
CTAGAAC7400.019.753
TAGAACT8100.019.413584
GTCTAGA7450.019.3691271
ATACACA11600.018.81896637
GTTCTAG6800.018.7720581
TATGCGG701.21985555E-418.55
AATTCGA500.007036923418.524
ACATGTA5350.017.289728
GCAGTCG4000.017.1125019
CTCTAGT7250.016.58620627
GTATCAA32500.016.5076922
ACGGAGT6400.016.47656226
AGTACTA904.449E-516.4444455
ACAACGG6550.016.3816823
AACGGAG6650.016.13533825
Produced by FastQC (version 0.11.5)